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Conference Paper: The Anti-Tumor Effects of M1 Macrophage-Loaded Poly(ethylene glycol) and Gelatin-Based Hydrogels on Hepatocellular Carcinoma
Title | The Anti-Tumor Effects of M1 Macrophage-Loaded Poly(ethylene glycol) and Gelatin-Based Hydrogels on Hepatocellular Carcinoma |
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Authors | |
Issue Date | 2017 |
Citation | 13th Asia Pacific Multidisciplinary Meeting for Cancer Research, The Chinese University of Hong Kong, Hong Kong, 22 June 2017 How to Cite? |
Abstract | Background and Aims: Recently we reported that direct injection of M1 macrophages significantly caused tumor regression in vivo. Despite the promising result, a major limitation in translating this approach is the induction of acute inflammatory response. To improve the strategy, a biocompatible scaffold for cell presentation and support is essential to control cell fate. Here, we aimed to elucidate the anti-tumor effects of a poly(ethylene glycol) diacrylate (PEGdA) and thiolated gelatin poly (ethylene glycol)(Gel-PEG-Cys) cross-linked hydrogels capsulated with M1 macrophages. Methods: Hydrogels were made at 0.5% (w/v) Iragcure 2959 photoinitiator, 10% (w/v) PEGdA, and 10% (w/v) Gel-PEG-Cys. Monocytic THP-1 cells were loaded into hydrogels and differentiated into M1 macrophages with lipopolysaccharide and interferon gamma. A nude mice ectopic liver cancer model with dorsal window chamber (DWC) and a subcutaneous tumor model were both performed to validate the in vivo application of M1 hydrogels. Results: M1 hydrogels significantly decreased the viability of HCC cells (MHCC97L: -46%; Hep3B: -56.9%; P<0.05) compared to the control in vitro. In response HCC cells, the hydrogel embedded M1 macrophages up-regulated nitrite and tumor necrosis factor alpha activating caspase-3 induced apoptosis in the tumor cells. Increased tumor necrosis was observed in DWC filled with M1 hydrogels. In addition, mice treated with M1 hydrogels exhibited a significant 2.4-fold decrease in signal intensity of subcutaneous HCC tumor compared to control (P=0.036). Conclusion: M1 hydrogels induced apoptosis in HCC cells and tumor regression in vivo. Continuous development of the scaffold-based cancer immunotherapy may provide an alternative and innovative strategy against HCC. |
Persistent Identifier | http://hdl.handle.net/10722/247962 |
DC Field | Value | Language |
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dc.contributor.author | Yeung, WH | - |
dc.contributor.author | Guerra, AD | - |
dc.contributor.author | Qi, X | - |
dc.contributor.author | Kao, WJ | - |
dc.contributor.author | Man, K | - |
dc.date.accessioned | 2017-10-18T08:35:34Z | - |
dc.date.available | 2017-10-18T08:35:34Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | 13th Asia Pacific Multidisciplinary Meeting for Cancer Research, The Chinese University of Hong Kong, Hong Kong, 22 June 2017 | - |
dc.identifier.uri | http://hdl.handle.net/10722/247962 | - |
dc.description.abstract | Background and Aims: Recently we reported that direct injection of M1 macrophages significantly caused tumor regression in vivo. Despite the promising result, a major limitation in translating this approach is the induction of acute inflammatory response. To improve the strategy, a biocompatible scaffold for cell presentation and support is essential to control cell fate. Here, we aimed to elucidate the anti-tumor effects of a poly(ethylene glycol) diacrylate (PEGdA) and thiolated gelatin poly (ethylene glycol)(Gel-PEG-Cys) cross-linked hydrogels capsulated with M1 macrophages. Methods: Hydrogels were made at 0.5% (w/v) Iragcure 2959 photoinitiator, 10% (w/v) PEGdA, and 10% (w/v) Gel-PEG-Cys. Monocytic THP-1 cells were loaded into hydrogels and differentiated into M1 macrophages with lipopolysaccharide and interferon gamma. A nude mice ectopic liver cancer model with dorsal window chamber (DWC) and a subcutaneous tumor model were both performed to validate the in vivo application of M1 hydrogels. Results: M1 hydrogels significantly decreased the viability of HCC cells (MHCC97L: -46%; Hep3B: -56.9%; P<0.05) compared to the control in vitro. In response HCC cells, the hydrogel embedded M1 macrophages up-regulated nitrite and tumor necrosis factor alpha activating caspase-3 induced apoptosis in the tumor cells. Increased tumor necrosis was observed in DWC filled with M1 hydrogels. In addition, mice treated with M1 hydrogels exhibited a significant 2.4-fold decrease in signal intensity of subcutaneous HCC tumor compared to control (P=0.036). Conclusion: M1 hydrogels induced apoptosis in HCC cells and tumor regression in vivo. Continuous development of the scaffold-based cancer immunotherapy may provide an alternative and innovative strategy against HCC. | - |
dc.language | eng | - |
dc.relation.ispartof | 13th Asia Pacific Multidisciplinary Meeting for Cancer Research | - |
dc.title | The Anti-Tumor Effects of M1 Macrophage-Loaded Poly(ethylene glycol) and Gelatin-Based Hydrogels on Hepatocellular Carcinoma | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yeung, WH: why21@hku.hk | - |
dc.identifier.email | Qi, X: qixiang515@connect.hku.hk | - |
dc.identifier.email | Kao, WJ: wjkao@hku.hk | - |
dc.identifier.email | Man, K: kwanman@hku.hk | - |
dc.identifier.authority | Kao, WJ=rp02076 | - |
dc.identifier.authority | Man, K=rp00417 | - |
dc.identifier.hkuros | 279733 | - |