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- Publisher Website: 10.1002/jbm.a.36084
- Scopus: eid_2-s2.0-85019093633
- PMID: 28380671
- WOS: WOS:000404370600010
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Article: Bicomponent fibrous scaffolds made through dual-source dual-power electrospinning: Dual delivery of rhBMP-2 and Ca-P nanoparticles and enhanced biological performances
Title | Bicomponent fibrous scaffolds made through dual-source dual-power electrospinning: Dual delivery of rhBMP-2 and Ca-P nanoparticles and enhanced biological performances |
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Authors | |
Keywords | Bicomponent scaffold Bone tissue engineering Ca-P nanoparticle Dual-source dual-power electrospinning rhBMP-2 |
Issue Date | 2017 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/ |
Citation | Journal of Biomedical Materials Research Part A, 2017, v. 105 n. 8, p. 2199-2209 How to Cite? |
Abstract | Electrospun scaffolds incorporated with both calcium phosphates (Ca-P) and bone morphogenetic protein-2 (BMP-2) have been used for bone tissue regeneration. However, in most cases BMP-2 and Ca-P were simply mixed and loaded in a monolithic structure, risking low BMP-2 loading level, reduced BMP-2 biological activity, uncontrolled BMP-2 release and inhomogeneous Ca-P distribution. In this investigation, novel bicomponent scaffolds having evenly distributed rhBMP-2-containing fibers and Ca-P nanoparticle-containing fibers were made using an established dual-source dual-power electrospinning technique with the assistance of emulsion electrospinning and blend electrospinning. The release behavior of rhBMP-2 and Ca2+ ions could be separately tuned and the released rhBMP-2 retained a 68% level for biological activity. MC3T3-E1 cells showed high viability and normal morphology on scaffolds. Compared to monocomponent scaffolds, enhanced cell proliferation, alkaline phosphatase activity, cell mineralization, and gene expression of osteogenic markers were achieved for bicomponent scaffolds due to the synergistic effect of rhBMP-2 and Ca-P nanoparticles. Bicomponent scaffolds with a double mass elicited further enhanced cell adhesion, spreading, proliferation, and osteogenic differentiation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2199–2209, 2017. |
Persistent Identifier | http://hdl.handle.net/10722/247486 |
ISSN | 2021 Impact Factor: 4.854 2020 SCImago Journal Rankings: 0.849 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, C | - |
dc.contributor.author | Lu, WW | - |
dc.contributor.author | Wang, M | - |
dc.date.accessioned | 2017-10-18T08:28:01Z | - |
dc.date.available | 2017-10-18T08:28:01Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Journal of Biomedical Materials Research Part A, 2017, v. 105 n. 8, p. 2199-2209 | - |
dc.identifier.issn | 1549-3296 | - |
dc.identifier.uri | http://hdl.handle.net/10722/247486 | - |
dc.description.abstract | Electrospun scaffolds incorporated with both calcium phosphates (Ca-P) and bone morphogenetic protein-2 (BMP-2) have been used for bone tissue regeneration. However, in most cases BMP-2 and Ca-P were simply mixed and loaded in a monolithic structure, risking low BMP-2 loading level, reduced BMP-2 biological activity, uncontrolled BMP-2 release and inhomogeneous Ca-P distribution. In this investigation, novel bicomponent scaffolds having evenly distributed rhBMP-2-containing fibers and Ca-P nanoparticle-containing fibers were made using an established dual-source dual-power electrospinning technique with the assistance of emulsion electrospinning and blend electrospinning. The release behavior of rhBMP-2 and Ca2+ ions could be separately tuned and the released rhBMP-2 retained a 68% level for biological activity. MC3T3-E1 cells showed high viability and normal morphology on scaffolds. Compared to monocomponent scaffolds, enhanced cell proliferation, alkaline phosphatase activity, cell mineralization, and gene expression of osteogenic markers were achieved for bicomponent scaffolds due to the synergistic effect of rhBMP-2 and Ca-P nanoparticles. Bicomponent scaffolds with a double mass elicited further enhanced cell adhesion, spreading, proliferation, and osteogenic differentiation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2199–2209, 2017. | - |
dc.language | eng | - |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304/ | - |
dc.relation.ispartof | Journal of Biomedical Materials Research Part A | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Bicomponent scaffold | - |
dc.subject | Bone tissue engineering | - |
dc.subject | Ca-P nanoparticle | - |
dc.subject | Dual-source dual-power electrospinning | - |
dc.subject | rhBMP-2 | - |
dc.title | Bicomponent fibrous scaffolds made through dual-source dual-power electrospinning: Dual delivery of rhBMP-2 and Ca-P nanoparticles and enhanced biological performances | - |
dc.type | Article | - |
dc.identifier.email | Lu, WW: wwlu@hku.hk | - |
dc.identifier.email | Wang, M: memwang@hku.hk | - |
dc.identifier.authority | Lu, WW=rp00411 | - |
dc.identifier.authority | Wang, M=rp00185 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/jbm.a.36084 | - |
dc.identifier.pmid | 28380671 | - |
dc.identifier.scopus | eid_2-s2.0-85019093633 | - |
dc.identifier.hkuros | 280234 | - |
dc.identifier.hkuros | 290764 | - |
dc.identifier.hkuros | 294745 | - |
dc.identifier.volume | 105 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 2199 | - |
dc.identifier.epage | 2209 | - |
dc.identifier.isi | WOS:000404370600010 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1549-3296 | - |