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Article: A Ni-NTA-based red fluorescence probe for protein labelling in live cells

TitleA Ni-NTA-based red fluorescence probe for protein labelling in live cells
Authors
CHAO, AJIANG, NYANG, YLi, HSun, H
Issue Date2017
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/tb#!recentarticles&all
Citation
Journal of Materials Chemistry B, 2017, v. 5, n. 6, p. 1166-1173 How to Cite?
DOI: http://dx.doi.org/10.1039/C6TB02848A
AbstractThe great success of a His6-Ni-nitrilotriaceate (Ni-NTA) system in protein purification has inspired scientists to develop novel Ni-NTA based fluoresent probes for imaging of proteins in live cells. Despite a great deal of effort being made, only very few such probes can cross the cell membrane to label intracellular proteins. Herein, a new red fluorescence probe (Ni-NTA-AB) consisting of a Ni-NTA moiety, BODIPY and an arylazide was designed and synthesized to selectively label His6-tagged proteins. Guided by the Ni-NTA, the probe selectively binds to proteins with a genetically fused His6-tag exemplified by a DNA repair protein, xerodermapigmentosum group A (XPA122), leading to ca. 26-fold fluorescence enhancement. Ni-NTA-AB could enter into live bacterial cells to label intracellular His6-tagged proteins with the assistance of Tween 80 and also to quantify His6-tagged proteins in SDS-PAGE upon photoactivation of arylazide with a wide linear range (25-1000 ng). This study may provide a new horizon for the multiple-channel tracking and labelling of His6-tagged proteins.
Persistent Identifierhttp://hdl.handle.net/10722/247281
ISSN
2050-750X
2021 Impact Factor: 7.571
2020 SCImago Journal Rankings: 1.316
ISI Accession Number ID
WOS:000395914000006

 

DC FieldValueLanguage
dc.contributor.authorCHAO, A-
dc.contributor.authorJIANG, N-
dc.contributor.authorYANG, Y-
dc.contributor.authorLi, H-
dc.contributor.authorSun, H-
dc.date.accessioned2017-10-18T08:24:59Z-
dc.date.available2017-10-18T08:24:59Z-
dc.date.issued2017-
dc.identifier.citationJournal of Materials Chemistry B, 2017, v. 5, n. 6, p. 1166-1173-
dc.identifier.issn2050-750X-
dc.identifier.urihttp://hdl.handle.net/10722/247281-
dc.description.abstractThe great success of a His6-Ni-nitrilotriaceate (Ni-NTA) system in protein purification has inspired scientists to develop novel Ni-NTA based fluoresent probes for imaging of proteins in live cells. Despite a great deal of effort being made, only very few such probes can cross the cell membrane to label intracellular proteins. Herein, a new red fluorescence probe (Ni-NTA-AB) consisting of a Ni-NTA moiety, BODIPY and an arylazide was designed and synthesized to selectively label His6-tagged proteins. Guided by the Ni-NTA, the probe selectively binds to proteins with a genetically fused His6-tag exemplified by a DNA repair protein, xerodermapigmentosum group A (XPA122), leading to ca. 26-fold fluorescence enhancement. Ni-NTA-AB could enter into live bacterial cells to label intracellular His6-tagged proteins with the assistance of Tween 80 and also to quantify His6-tagged proteins in SDS-PAGE upon photoactivation of arylazide with a wide linear range (25-1000 ng). This study may provide a new horizon for the multiple-channel tracking and labelling of His6-tagged proteins.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/tb#!recentarticles&all-
dc.relation.ispartofJournal of Materials Chemistry B-
dc.titleA Ni-NTA-based red fluorescence probe for protein labelling in live cells-
dc.typeArticle-
dc.identifier.emailLi, H: hylichem@hku.hk-
dc.identifier.emailSun, H: hsun@hku.hk-
dc.identifier.authoritySun, H=rp00777-
dc.identifier.doi10.1039/C6TB02848A-
dc.identifier.pmid32263587-
dc.identifier.scopuseid_2-s2.0-85012053657-
dc.identifier.hkuros281613-
dc.identifier.volume5-
dc.identifier.issue6-
dc.identifier.spage1166-
dc.identifier.epage1173-
dc.identifier.isiWOS:000395914000006-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2050-750X-

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