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Article: Nanoparticle-encapsulated baicalein markedly modulates pro-inflammatory response in gingival epithelial cells

TitleNanoparticle-encapsulated baicalein markedly modulates pro-inflammatory response in gingival epithelial cells
Authors
Issue Date2017
PublisherRSC Publications. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/nr#!recentarticles&all
Citation
Nanoscale, 2017, v. 9, p. 12897-12907 How to Cite?
AbstractSevere gum disease (periodontitis), which is one of the major global oral diseases, results from microbe-host dysbiosis and dysregulated immuno-inflammatory responses. It seriously affects oral health and general wellbeing with significant socio-economic implications. It has been well documented that natural flavonoids such as baicalin (BA) and baicalein (BE) possess potent anti-inflammatory effects. However, their intrinsic poor solubility and low bioavailability severely limit their biomedical applications. In the present study, BA and BE were encapsulated in our synthesized and amine-modified mesoporous silica nanoparticles (MSNs) (Nano-BA and Nano-BE, respectively), and their loading efficiencies and releasing profiles were investigated. Their cytotoxicity was examined on primary human gingival epithelial cells (hGECs), and the cellular uptake of Nano-BA or Nano-BE was visualized via a transmission electron microscope. Their anti-inflammatory effects were evaluated in IL-1β-treated hGECs using the cytokine array and enzyme-linked immunosorbent assay. The present study shows that the amine-modified MSNs could encapsulate BA and BE, and nano-encapsulation greatly enhances the drug delivery rate and prolongs the release of BA and BE up to 216 h. Moreover, both Nano-BA and Nano-BE could be internalized by hGECs and retained intracellularly in nanoparticle-free media for at least 24 h. Note that Nano-BE pre-treatment effectively down-regulates the IL-1β-induced expression of IL-6 and IL-8 in hGECs. In conclusion, nanoparticle-encapsulated BE exhibits notable anti-inflammatory effects through effective release and cellular internalization approaches. This study may facilitate the development of novel drug delivery systems for improving oral care.
Persistent Identifierhttp://hdl.handle.net/10722/247223
ISSN
2020 Impact Factor: 7.79
2020 SCImago Journal Rankings: 2.038
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLI, X-
dc.contributor.authorLuo, W-
dc.contributor.authorNg, TC-
dc.contributor.authorLeung, PC-
dc.contributor.authorZhang, C-
dc.contributor.authorLeung, CFK-
dc.contributor.authorJin, L-
dc.date.accessioned2017-10-18T08:24:09Z-
dc.date.available2017-10-18T08:24:09Z-
dc.date.issued2017-
dc.identifier.citationNanoscale, 2017, v. 9, p. 12897-12907-
dc.identifier.issn2040-3364-
dc.identifier.urihttp://hdl.handle.net/10722/247223-
dc.description.abstractSevere gum disease (periodontitis), which is one of the major global oral diseases, results from microbe-host dysbiosis and dysregulated immuno-inflammatory responses. It seriously affects oral health and general wellbeing with significant socio-economic implications. It has been well documented that natural flavonoids such as baicalin (BA) and baicalein (BE) possess potent anti-inflammatory effects. However, their intrinsic poor solubility and low bioavailability severely limit their biomedical applications. In the present study, BA and BE were encapsulated in our synthesized and amine-modified mesoporous silica nanoparticles (MSNs) (Nano-BA and Nano-BE, respectively), and their loading efficiencies and releasing profiles were investigated. Their cytotoxicity was examined on primary human gingival epithelial cells (hGECs), and the cellular uptake of Nano-BA or Nano-BE was visualized via a transmission electron microscope. Their anti-inflammatory effects were evaluated in IL-1β-treated hGECs using the cytokine array and enzyme-linked immunosorbent assay. The present study shows that the amine-modified MSNs could encapsulate BA and BE, and nano-encapsulation greatly enhances the drug delivery rate and prolongs the release of BA and BE up to 216 h. Moreover, both Nano-BA and Nano-BE could be internalized by hGECs and retained intracellularly in nanoparticle-free media for at least 24 h. Note that Nano-BE pre-treatment effectively down-regulates the IL-1β-induced expression of IL-6 and IL-8 in hGECs. In conclusion, nanoparticle-encapsulated BE exhibits notable anti-inflammatory effects through effective release and cellular internalization approaches. This study may facilitate the development of novel drug delivery systems for improving oral care.-
dc.languageeng-
dc.publisherRSC Publications. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/nr#!recentarticles&all-
dc.relation.ispartofNanoscale-
dc.titleNanoparticle-encapsulated baicalein markedly modulates pro-inflammatory response in gingival epithelial cells-
dc.typeArticle-
dc.identifier.emailLuo, W: lwhku@hku.hk-
dc.identifier.emailNg, TC: tcng5uid@hku.hk-
dc.identifier.emailZhang, C: zhangcf@hku.hk-
dc.identifier.emailLeung, CFK: kcfleung@hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.authorityZhang, C=rp01408-
dc.identifier.authorityJin, L=rp00028-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/C7NR02546G-
dc.identifier.pmid28650029-
dc.identifier.scopuseid_2-s2.0-85029527915-
dc.identifier.hkuros279363-
dc.identifier.volume9-
dc.identifier.spage12897-
dc.identifier.epage12907-
dc.identifier.isiWOS:000410659800012-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2040-3364-

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