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Article: Polyphyletic origin of MERS coronaviruses and isolation of a novel clade A strain from dromedary camels in the United Arab Emirates

TitlePolyphyletic origin of MERS coronaviruses and isolation of a novel clade A strain from dromedary camels in the United Arab Emirates
Authors
Lau, SKPWernery, RWong, YMJoseph, STsang, KLPatteril, NAElizabeth, SKChan, KHMuhammed, RKinne, JYuen, KYWernery, UWoo, PCY
KeywordsClade A
Dromedary camels
MERS
Middle East respiratory syndrome coronavirus
Novel
Polyphyletic
United Arab Emirates
Issue Date2016
PublisherNature Publishing Group for Shanghai Shangyixun Cultural Communication Company. The Journal's web site is located at http://www.nature.com/emi/marketing/index.html
Citation
Emerging Microbes & Infections, 2016, v. 5, article no. e128 How to Cite?
DOI: http://dx.doi.org/10.1038/emi.2016.129
AbstractLittle is known regarding the molecular epidemiology of Middle East respiratory syndrome coronavirus (MERS-CoV) circulating in dromedaries outside Saudi Arabia. To address this knowledge gap, we sequenced 10 complete genomes of MERS-CoVs isolated from 2 live and 8 dead dromedaries from different regions in the United Arab Emirates (UAE). Phylogenetic analysis revealed one novel clade A strain, the first detected in the UAE, and nine clade B strains. Strain D998/15 had a distinct phylogenetic position within clade A, being more closely related to the dromedary isolate NRCE-HKU205 from Egypt than to the human isolates EMC/2012 and Jordan-N3/2012. A comparison of predicted protein sequences also demonstrated the existence of two clade A lineages with unique amino acid substitutions, A1 (EMC/2012 and Jordan-N3/2012) and A2 (D998/15 and NRCE-HKU205), circulating in humans and camels, respectively. The nine clade B isolates belong to three distinct lineages: B1, B3 and B5. Two B3 strains, D1271/15 and D1189.1/15, showed evidence of recombination between lineages B4 and B5 in ORF1ab. Molecular clock analysis dated the time of the most recent common ancestor (tMRCA) of clade A to March 2011 and that of clade B to November 2011. Our data support a polyphyletic origin of MERS-CoV in dromedaries and the co-circulation of diverse MERS-CoVs including recombinant strains in the UAE.
Persistent Identifierhttp://hdl.handle.net/10722/245139
ISSN
2222-1751
2021 Impact Factor: 19.568
2020 SCImago Journal Rankings: 2.475
PubMed Central ID
PMC5180373
ISI Accession Number ID
WOS:000391507900004

 

DC FieldValueLanguage
dc.contributor.authorLau, SKP-
dc.contributor.authorWernery, R-
dc.contributor.authorWong, YM-
dc.contributor.authorJoseph, S-
dc.contributor.authorTsang, KL-
dc.contributor.authorPatteril, NA-
dc.contributor.authorElizabeth, SK-
dc.contributor.authorChan, KH-
dc.contributor.authorMuhammed, R-
dc.contributor.authorKinne, J-
dc.contributor.authorYuen, KY-
dc.contributor.authorWernery, U-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2017-09-18T02:05:19Z-
dc.date.available2017-09-18T02:05:19Z-
dc.date.issued2016-
dc.identifier.citationEmerging Microbes & Infections, 2016, v. 5, article no. e128-
dc.identifier.issn2222-1751-
dc.identifier.urihttp://hdl.handle.net/10722/245139-
dc.description.abstractLittle is known regarding the molecular epidemiology of Middle East respiratory syndrome coronavirus (MERS-CoV) circulating in dromedaries outside Saudi Arabia. To address this knowledge gap, we sequenced 10 complete genomes of MERS-CoVs isolated from 2 live and 8 dead dromedaries from different regions in the United Arab Emirates (UAE). Phylogenetic analysis revealed one novel clade A strain, the first detected in the UAE, and nine clade B strains. Strain D998/15 had a distinct phylogenetic position within clade A, being more closely related to the dromedary isolate NRCE-HKU205 from Egypt than to the human isolates EMC/2012 and Jordan-N3/2012. A comparison of predicted protein sequences also demonstrated the existence of two clade A lineages with unique amino acid substitutions, A1 (EMC/2012 and Jordan-N3/2012) and A2 (D998/15 and NRCE-HKU205), circulating in humans and camels, respectively. The nine clade B isolates belong to three distinct lineages: B1, B3 and B5. Two B3 strains, D1271/15 and D1189.1/15, showed evidence of recombination between lineages B4 and B5 in ORF1ab. Molecular clock analysis dated the time of the most recent common ancestor (tMRCA) of clade A to March 2011 and that of clade B to November 2011. Our data support a polyphyletic origin of MERS-CoV in dromedaries and the co-circulation of diverse MERS-CoVs including recombinant strains in the UAE.-
dc.languageeng-
dc.publisherNature Publishing Group for Shanghai Shangyixun Cultural Communication Company. The Journal's web site is located at http://www.nature.com/emi/marketing/index.html-
dc.relation.ispartofEmerging Microbes & Infections-
dc.subjectClade A-
dc.subjectDromedary camels-
dc.subjectMERS-
dc.subjectMiddle East respiratory syndrome coronavirus-
dc.subjectNovel-
dc.subjectPolyphyletic-
dc.subjectUnited Arab Emirates-
dc.titlePolyphyletic origin of MERS coronaviruses and isolation of a novel clade A strain from dromedary camels in the United Arab Emirates-
dc.typeArticle-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWong, YM: emilyhk@hku.hk-
dc.identifier.emailChan, KH: chankh2@hkucc.hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityChan, KH=rp01921-
dc.identifier.authorityYuen, KY=rp00366-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/emi.2016.129-
dc.identifier.pmcidPMC5180373-
dc.identifier.scopuseid_2-s2.0-85011966022-
dc.identifier.hkuros275886-
dc.identifier.volume5-
dc.identifier.spagearticle no. e128-
dc.identifier.epagearticle no. e128-
dc.identifier.isiWOS:000391507900004-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2222-1751-

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