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Article: Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

TitleModeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
Authors
KeywordsBioengineering
Issue 120
Ovarian cancer
Microfluidics
Shear stress
Peritoneal mesothelial cells
Multicellular spheroids
3D culture
Co-culture
Issue Date2017
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com
Citation
Journal of Visualized Experiments, 2017, n. 120, article no. e55337 How to Cite?
AbstractOvarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.
Persistent Identifierhttp://hdl.handle.net/10722/245087
ISSN
2023 Impact Factor: 1.2
2023 SCImago Journal Rankings: 0.449
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, S-
dc.contributor.authorIp, CKM-
dc.contributor.authorTang, MYH-
dc.contributor.authorSy, SKH-
dc.contributor.authorYung, S-
dc.contributor.authorChan, TM-
dc.contributor.authorYang, M-
dc.contributor.authorShum, HC-
dc.contributor.authorWong, AST-
dc.date.accessioned2017-09-18T02:04:23Z-
dc.date.available2017-09-18T02:04:23Z-
dc.date.issued2017-
dc.identifier.citationJournal of Visualized Experiments, 2017, n. 120, article no. e55337-
dc.identifier.issn1940-087X-
dc.identifier.urihttp://hdl.handle.net/10722/245087-
dc.description.abstractOvarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.-
dc.languageeng-
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com-
dc.relation.ispartofJournal of Visualized Experiments-
dc.rightsCopyright © 2017 Journal of Visualized Experiments.-
dc.subjectBioengineering-
dc.subjectIssue 120-
dc.subjectOvarian cancer-
dc.subjectMicrofluidics-
dc.subjectShear stress-
dc.subjectPeritoneal mesothelial cells-
dc.subjectMulticellular spheroids-
dc.subject3D culture-
dc.subjectCo-culture-
dc.titleModeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice-
dc.typeArticle-
dc.identifier.emailLi, S: lss33@HKUCC-COM.hku.hk-
dc.identifier.emailTang, MYH: sdmt99@connect.hku.hk-
dc.identifier.emailYung, S: ssyyung@hku.hk-
dc.identifier.emailChan, TM: dtmchan@hkucc.hku.hk-
dc.identifier.emailShum, HC: ashum@hku.hk-
dc.identifier.emailWong, AST: awong1@hku.hk-
dc.identifier.authorityYung, S=rp00455-
dc.identifier.authorityChan, TM=rp00394-
dc.identifier.authorityShum, HC=rp01439-
dc.identifier.authorityWong, AST=rp00805-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3791/55337-
dc.identifier.pmid28287578-
dc.identifier.pmcidPMC5409276-
dc.identifier.scopuseid_2-s2.0-85014328084-
dc.identifier.hkuros278681-
dc.identifier.issue120-
dc.identifier.spagearticle no. e55337-
dc.identifier.epagearticle no. e55337-
dc.identifier.isiWOS:000397847700086-
dc.publisher.placeUnited States-
dc.identifier.issnl1940-087X-

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