File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Hypoxic preconditioning of marrow-derived progenitor cells as a source for the generation of mature Schwann cells

TitleHypoxic preconditioning of marrow-derived progenitor cells as a source for the generation of mature Schwann cells
Authors
KeywordsDevelopmental Biology
Issue 124
Marrow stromal cell
Cell therapy
Schwann cell
Hypoxia
Spinal cord injury
Cell differentiation
Issue Date2017
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com
Citation
Journal of Visualized Experiments, 2017, n. 124, article no. e55794 How to Cite?
AbstractThis manuscript describes a means to enrich for neural progenitors from the marrow stromal cell (MSC) population and thereafter to direct them to the mature Schwann cell fate. We subjected rat and human MSCs to transient hypoxic conditions (1% oxygen for 16 h) followed by expansion as neurospheres upon low-attachment substratum with epidermal growth factor (EGF)/basic fibroblast growth factor (bFGF) supplementation. Neurospheres were seeded onto poly-D-lysine/laminin-coated tissue culture plastic and cultured in a gliogenic cocktail containing β-Heregulin, bFGF, and platelet-derived growth factor (PDGF) to generate Schwann cell-like cells (SCLCs). SCLCs were directed to fate commitment via coculture for 2 weeks with purified dorsal root ganglia (DRG) neurons obtained from E14-15 pregnant Sprague Dawley rats. Mature Schwann cells demonstrate persistence in S100β/p75 expression and can form myelin segments. Cells generated in this manner have potential applications in autologous cell transplantation following spinal cord injury, as well as in disease modeling.
Persistent Identifierhttp://hdl.handle.net/10722/244676
ISSN
2021 Impact Factor: 1.424
2020 SCImago Journal Rankings: 0.596
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsui, YP-
dc.contributor.authorMung, AKL-
dc.contributor.authorChan, YS-
dc.contributor.authorShum, DKY-
dc.contributor.authorShea, GKH-
dc.date.accessioned2017-09-18T01:57:01Z-
dc.date.available2017-09-18T01:57:01Z-
dc.date.issued2017-
dc.identifier.citationJournal of Visualized Experiments, 2017, n. 124, article no. e55794-
dc.identifier.issn1940-087X-
dc.identifier.urihttp://hdl.handle.net/10722/244676-
dc.description.abstractThis manuscript describes a means to enrich for neural progenitors from the marrow stromal cell (MSC) population and thereafter to direct them to the mature Schwann cell fate. We subjected rat and human MSCs to transient hypoxic conditions (1% oxygen for 16 h) followed by expansion as neurospheres upon low-attachment substratum with epidermal growth factor (EGF)/basic fibroblast growth factor (bFGF) supplementation. Neurospheres were seeded onto poly-D-lysine/laminin-coated tissue culture plastic and cultured in a gliogenic cocktail containing β-Heregulin, bFGF, and platelet-derived growth factor (PDGF) to generate Schwann cell-like cells (SCLCs). SCLCs were directed to fate commitment via coculture for 2 weeks with purified dorsal root ganglia (DRG) neurons obtained from E14-15 pregnant Sprague Dawley rats. Mature Schwann cells demonstrate persistence in S100β/p75 expression and can form myelin segments. Cells generated in this manner have potential applications in autologous cell transplantation following spinal cord injury, as well as in disease modeling.-
dc.languageeng-
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com-
dc.relation.ispartofJournal of Visualized Experiments-
dc.rightsCopyright © 2017 Journal of Visualized Experiments.-
dc.subjectDevelopmental Biology-
dc.subjectIssue 124-
dc.subjectMarrow stromal cell-
dc.subjectCell therapy-
dc.subjectSchwann cell-
dc.subjectHypoxia-
dc.subjectSpinal cord injury-
dc.subjectCell differentiation-
dc.titleHypoxic preconditioning of marrow-derived progenitor cells as a source for the generation of mature Schwann cells-
dc.typeArticle-
dc.identifier.emailTsui, YP: alex2013@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.emailShea, GKH: gkshea@hku.hk-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.authorityShea, GKH=rp01781-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3791/55794-
dc.identifier.pmid28654046-
dc.identifier.pmcidPMC5608432-
dc.identifier.scopuseid_2-s2.0-85021227864-
dc.identifier.hkuros278108-
dc.identifier.issue124-
dc.identifier.spagearticle no. e55794-
dc.identifier.epagearticle no. e55794-
dc.identifier.isiWOS:000407448100050-
dc.publisher.placeUnited States-
dc.identifier.issnl1940-087X-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats