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- Publisher Website: 10.1152/ajpcell.00154.2005
- Scopus: eid_2-s2.0-26844531180
- PMID: 15972839
- WOS: WOS:000232385300006
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Article: Age-related apoptotic responses to stretch-induced hypertrophy in quail slow-tonic skeletal muscle
Title | Age-related apoptotic responses to stretch-induced hypertrophy in quail slow-tonic skeletal muscle |
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Authors | |
Keywords | Bax Sarcopenia Aging Apoptosis-inducing factor Heat shock proteins Bcl-2 |
Issue Date | 2005 |
Citation | American Journal of Physiology - Cell Physiology, 2005, v. 289, n. 5 58-5 How to Cite? |
Abstract | In the present study, we examined the responses of apoptosis and apoptotic regulatory factors to muscle hypertrophy induced by stretch overload in quail slow-tonic muscles. The wings from one side of young and aged Japanese quails were loaded by attaching a tube weight corresponding to 12% of the bird's body weight for 7 or 21 days. Muscle from the contralateral side served as the intraanimal control. Relative to the intraanimal contralateral control side, the muscle wet weight increased by 96% in young birds, whereas the muscle weight gain in aged birds was not significant after 7 days of loading. After 21 days of loading, muscle weight significantly increased by 179% and 102% in young and aged birds, respectively. Heat shock protein (HSP)72 and HSP27 protein contents in the loaded sides were higher than on the control sides exclusively in young birds after 7 days of loading. Compared with the contralateral control muscle, the extent of apoptotic DNA fragmentation and the total cytosolic apoptosis-inducing factor protein content were reduced in all loaded muscles except for the 7-day-loaded muscles from the aged birds. Bax protein content was diminished in the loaded muscle relative to the control side from all groups, whereas Bcl-2 protein content was reduced in the young and aged muscles after 21 days of loading. The total cytosolic cytochrome c protein content was decreased and the X chromosome-linked inhibitor of apoptosis protein content was elevated in 7- and 21-day-loaded muscles relative to the intraanimal control muscle from young birds. Furthermore, after 7 days of loading the muscles of aged birds, H 2 O 2 content and the total cytosolic protein content of second mitochondrial activator of caspases/direct inhibitor of apoptosis-binding protein with low isoelectric point were elevated compared with the intraanimal control side. These data suggest that stretch overload-induced muscle hypertrophy is associated with changes in apoptosis in slow-tonic skeletal muscle. Moreover, discrepant apoptotic responses to muscle overload in young and aged muscles may account in part for the age-related decline in the capability for muscle hypertrophy. Copyright © 2005 the American Physiological Society. |
Persistent Identifier | http://hdl.handle.net/10722/244261 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.711 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Siu, Parco M. | - |
dc.contributor.author | Alway, Stephen E. | - |
dc.date.accessioned | 2017-08-31T08:56:30Z | - |
dc.date.available | 2017-08-31T08:56:30Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | American Journal of Physiology - Cell Physiology, 2005, v. 289, n. 5 58-5 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.uri | http://hdl.handle.net/10722/244261 | - |
dc.description.abstract | In the present study, we examined the responses of apoptosis and apoptotic regulatory factors to muscle hypertrophy induced by stretch overload in quail slow-tonic muscles. The wings from one side of young and aged Japanese quails were loaded by attaching a tube weight corresponding to 12% of the bird's body weight for 7 or 21 days. Muscle from the contralateral side served as the intraanimal control. Relative to the intraanimal contralateral control side, the muscle wet weight increased by 96% in young birds, whereas the muscle weight gain in aged birds was not significant after 7 days of loading. After 21 days of loading, muscle weight significantly increased by 179% and 102% in young and aged birds, respectively. Heat shock protein (HSP)72 and HSP27 protein contents in the loaded sides were higher than on the control sides exclusively in young birds after 7 days of loading. Compared with the contralateral control muscle, the extent of apoptotic DNA fragmentation and the total cytosolic apoptosis-inducing factor protein content were reduced in all loaded muscles except for the 7-day-loaded muscles from the aged birds. Bax protein content was diminished in the loaded muscle relative to the control side from all groups, whereas Bcl-2 protein content was reduced in the young and aged muscles after 21 days of loading. The total cytosolic cytochrome c protein content was decreased and the X chromosome-linked inhibitor of apoptosis protein content was elevated in 7- and 21-day-loaded muscles relative to the intraanimal control muscle from young birds. Furthermore, after 7 days of loading the muscles of aged birds, H 2 O 2 content and the total cytosolic protein content of second mitochondrial activator of caspases/direct inhibitor of apoptosis-binding protein with low isoelectric point were elevated compared with the intraanimal control side. These data suggest that stretch overload-induced muscle hypertrophy is associated with changes in apoptosis in slow-tonic skeletal muscle. Moreover, discrepant apoptotic responses to muscle overload in young and aged muscles may account in part for the age-related decline in the capability for muscle hypertrophy. Copyright © 2005 the American Physiological Society. | - |
dc.language | eng | - |
dc.relation.ispartof | American Journal of Physiology - Cell Physiology | - |
dc.subject | Bax | - |
dc.subject | Sarcopenia | - |
dc.subject | Aging | - |
dc.subject | Apoptosis-inducing factor | - |
dc.subject | Heat shock proteins | - |
dc.subject | Bcl-2 | - |
dc.title | Age-related apoptotic responses to stretch-induced hypertrophy in quail slow-tonic skeletal muscle | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1152/ajpcell.00154.2005 | - |
dc.identifier.pmid | 15972839 | - |
dc.identifier.scopus | eid_2-s2.0-26844531180 | - |
dc.identifier.volume | 289 | - |
dc.identifier.issue | 5 58-5 | - |
dc.identifier.spage | null | - |
dc.identifier.epage | null | - |
dc.identifier.isi | WOS:000232385300006 | - |
dc.identifier.issnl | 0363-6143 | - |