Differential effects of low-magnitude high-frequency vibration on reloading hind-limb soleus and gastrocnemius medialis muscles in 28-day tail-suspended rats

Abstract

© 2015, International Society of Musculoskeletal and Neuronal Interactions. All rights reserved. Objectives: Low-magnitude high-frequency vibration (LMHFV) was reported beneficial to muscle contractile functions in clinical and preclinical studies. This study aims to investigate the effects of LMHFV on myofibers, myogenic cells and functional properties of disused soleus (Sol) and gastrocnemius medialis (GM) during reloading. Methods: Sprague Dawley rats were hind-limb unloaded for 28 days and assigned to reloading control (Ctrl) or LMHFV group (Vib). Sol and GM of both groups were harvested for fiber typing, proliferating myogenic cell counting and in vitro functional assessment. Results:Myogenic cells proliferation was promoted by LMHFV in both Sol and GM (p < 0.001 and p < 0.05 respectively). Force generating capacity was not much affected (Vib=Ctrl, p > 0.05) but fast-fiber favorable changes in fiber type switching (more type IIA but lower type I in Vib; p < 0.05 and 0.01 respectively) and fiber hypertrophy (type I, Vib < Ctrl; p < 0.01) were observed mainly in GM. Conclusion: LMHFV was not detrimental to reloading muscles but the outcomes were muscle dependent. The unique fiber type composition and anatomical differences between Sol and GM might render the differential muscle responses to LMHFV. Further investigations on myofibers type specific responses to different LMHFV regimes and myogenic cell interaction with associated myofiber were proposed.