File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3389/fphys.2015.00293
- Scopus: eid_2-s2.0-84946575216
Supplementary
-
Citations:
- Scopus: 0
- Appears in Collections:
Article: SIRT1-dependent myoprotective effects of resveratrol on muscle injury induced by compression
Title | SIRT1-dependent myoprotective effects of resveratrol on muscle injury induced by compression |
---|---|
Authors | |
Keywords | Compression injury Skeletal muscle SIRT1 Resveratrol Pressure ulcer |
Issue Date | 2015 |
Citation | Frontiers in Physiology, 2015, v. 6, n. OCT How to Cite? |
Abstract | © 2015 Sin, Yung, Yip, Chan, Wong, Tam and Siu. Our current understanding on the molecular mechanisms by which sustained compression induces skeletal muscle injury is very limited. This study aimed to test the hypothesis that activation of SIRT1 by the natural antioxidant resveratrol could deactivate apoptotic and catabolic signaling in skeletal muscle exposed to moderate compression. Two cycles of 6-h constant pressure at 100 mmHg was applied to the tibialis region of right, but not left hindlimbs of Sprague Dawley rats pre-treated with DMSO (vehicle control) or resveratrol with/without sirtinol. Skeletal muscle tissues lying underneath and spatially corresponding to the compressed sites were collected for analyses. Resveratrol prevented the compression-induced manifestations of pathohistological damages including elevations of the number of interstitial nuclei and area of interstitial space and ameliorated oxidative damages measured as 4-hydroxy-2-nonenal (4HNE) and nitrotyrosine in skeletal muscle. In parallel, resveratrol augmented the expression level and activity of SIRT1 and phosphorylation levels of Foxo3a and Akt while suppressed the increases in protein abundances of p53, Bax, MAFbx, and ubiquitin, enzymatic activities of caspase 3 and 20S proteasome, and apoptotic DNA fragmentation in the compressed muscle. These favorable myoprotective effects of resveratrol were diminished upon pharmacological blockade of SIRT1 by using sirtinol. These novel data support the hypothesis that the anti-apoptotic and anti-catabolic effects of resveratrol on compression injury in skeletal muscle required the action of SIRT1. |
Persistent Identifier | http://hdl.handle.net/10722/244210 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sin, Thomas K. | - |
dc.contributor.author | Yung, Benjamin Y. | - |
dc.contributor.author | Yip, Shea P. | - |
dc.contributor.author | Chan, Lawrence W. | - |
dc.contributor.author | Wong, Cesar S. | - |
dc.contributor.author | Tam, Eric W. | - |
dc.contributor.author | Siu, Parco M. | - |
dc.date.accessioned | 2017-08-31T08:56:21Z | - |
dc.date.available | 2017-08-31T08:56:21Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Frontiers in Physiology, 2015, v. 6, n. OCT | - |
dc.identifier.uri | http://hdl.handle.net/10722/244210 | - |
dc.description.abstract | © 2015 Sin, Yung, Yip, Chan, Wong, Tam and Siu. Our current understanding on the molecular mechanisms by which sustained compression induces skeletal muscle injury is very limited. This study aimed to test the hypothesis that activation of SIRT1 by the natural antioxidant resveratrol could deactivate apoptotic and catabolic signaling in skeletal muscle exposed to moderate compression. Two cycles of 6-h constant pressure at 100 mmHg was applied to the tibialis region of right, but not left hindlimbs of Sprague Dawley rats pre-treated with DMSO (vehicle control) or resveratrol with/without sirtinol. Skeletal muscle tissues lying underneath and spatially corresponding to the compressed sites were collected for analyses. Resveratrol prevented the compression-induced manifestations of pathohistological damages including elevations of the number of interstitial nuclei and area of interstitial space and ameliorated oxidative damages measured as 4-hydroxy-2-nonenal (4HNE) and nitrotyrosine in skeletal muscle. In parallel, resveratrol augmented the expression level and activity of SIRT1 and phosphorylation levels of Foxo3a and Akt while suppressed the increases in protein abundances of p53, Bax, MAFbx, and ubiquitin, enzymatic activities of caspase 3 and 20S proteasome, and apoptotic DNA fragmentation in the compressed muscle. These favorable myoprotective effects of resveratrol were diminished upon pharmacological blockade of SIRT1 by using sirtinol. These novel data support the hypothesis that the anti-apoptotic and anti-catabolic effects of resveratrol on compression injury in skeletal muscle required the action of SIRT1. | - |
dc.language | eng | - |
dc.relation.ispartof | Frontiers in Physiology | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Compression injury | - |
dc.subject | Skeletal muscle | - |
dc.subject | SIRT1 | - |
dc.subject | Resveratrol | - |
dc.subject | Pressure ulcer | - |
dc.title | SIRT1-dependent myoprotective effects of resveratrol on muscle injury induced by compression | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3389/fphys.2015.00293 | - |
dc.identifier.scopus | eid_2-s2.0-84946575216 | - |
dc.identifier.volume | 6 | - |
dc.identifier.issue | OCT | - |
dc.identifier.spage | null | - |
dc.identifier.epage | null | - |
dc.identifier.eissn | 1664-042X | - |
dc.identifier.issnl | 1664-042X | - |