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Article: ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology

TitleATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology
Authors
Issue Date2017
PublishereLife Sciences Publications Ltd. The Journal's web site is located at http://elifesciences.org/
Citation
eLife, 2017, v. 6, p. e26770:1-29 How to Cite?
AbstractGrowth and development of plants is ultimately driven by light energy captured through photosynthesis. ATP acts as universal cellular energy cofactor fuelling all life processes, including gene expression, metabolism, and transport. Despite a mechanistic understanding of ATP biochemistry, ATP dynamics in the living plant have been largely elusive. Here, we establish MgATP2- measurement in living plants using the fluorescent protein biosensor ATeam1.03-nD/nA. We generate Arabidopsis sensor lines and investigate the sensor in vitro under conditions appropriate for the plant cytosol. We establish an assay for ATP fluxes in isolated mitochondria, and demonstrate that the sensor responds rapidly and reliably to MgATP2- changes in planta. A MgATP2- map of the Arabidopsis seedling highlights different MgATP2- concentrations between tissues and within individual cell types, such as root hairs. Progression of hypoxia reveals substantial plasticity of ATP homeostasis in seedlings, demonstrating that ATP dynamics can be monitored in the living plant.
Persistent Identifierhttp://hdl.handle.net/10722/243552
ISSN
2019 Impact Factor: 7.08
2015 SCImago Journal Rankings: 6.356

 

DC FieldValueLanguage
dc.contributor.authorDe Col, V-
dc.contributor.authorPhilippe, F-
dc.contributor.authorNietzel, T-
dc.contributor.authorElsasser, M-
dc.contributor.authorVOON, CP-
dc.contributor.authorCandeo, A-
dc.contributor.authorSeeliger, I-
dc.contributor.authorFricker, MD-
dc.contributor.authorGrefen, C-
dc.contributor.authorMoller, IM-
dc.contributor.authorBassi, A-
dc.contributor.authorLim, BL-
dc.contributor.authorZancani, M-
dc.contributor.authorMeyer, AJ-
dc.contributor.authorCosta, A-
dc.contributor.authorWagner, S-
dc.contributor.authorSchwarzlander, M-
dc.date.accessioned2017-08-25T02:56:22Z-
dc.date.available2017-08-25T02:56:22Z-
dc.date.issued2017-
dc.identifier.citationeLife, 2017, v. 6, p. e26770:1-29-
dc.identifier.issn2050-084X-
dc.identifier.urihttp://hdl.handle.net/10722/243552-
dc.description.abstractGrowth and development of plants is ultimately driven by light energy captured through photosynthesis. ATP acts as universal cellular energy cofactor fuelling all life processes, including gene expression, metabolism, and transport. Despite a mechanistic understanding of ATP biochemistry, ATP dynamics in the living plant have been largely elusive. Here, we establish MgATP2- measurement in living plants using the fluorescent protein biosensor ATeam1.03-nD/nA. We generate Arabidopsis sensor lines and investigate the sensor in vitro under conditions appropriate for the plant cytosol. We establish an assay for ATP fluxes in isolated mitochondria, and demonstrate that the sensor responds rapidly and reliably to MgATP2- changes in planta. A MgATP2- map of the Arabidopsis seedling highlights different MgATP2- concentrations between tissues and within individual cell types, such as root hairs. Progression of hypoxia reveals substantial plasticity of ATP homeostasis in seedlings, demonstrating that ATP dynamics can be monitored in the living plant.-
dc.languageeng-
dc.publishereLife Sciences Publications Ltd. The Journal's web site is located at http://elifesciences.org/-
dc.relation.ispartofeLife-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology-
dc.typeArticle-
dc.identifier.emailLim, BL: bllim@hkucc.hku.hk-
dc.identifier.authorityLim, BL=rp00744-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.7554/eLife.26770-
dc.identifier.hkuros274006-
dc.identifier.volume6-
dc.identifier.spagee26770:1-
dc.identifier.epage29-
dc.publisher.placeCambridge, UK-
dc.identifier.f1000727817135-

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