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- Publisher Website: 10.1099/jgv.0.000717
- Scopus: eid_2-s2.0-85017011014
- PMID: 28113045
- WOS: WOS:000399235600008
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Article: PAN substitutions A37S, A37S/I61T and A37S/V63I attenuate the replication of H7N7 influenza A virus by impairing the polymerase and endonuclease activities
Title | PAN substitutions A37S, A37S/I61T and A37S/V63I attenuate the replication of H7N7 influenza A virus by impairing the polymerase and endonuclease activities |
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Authors | |
Keywords | Influenza A virus PAN-A37S PAN-A37S/I61T PAN-A37S/V63I Polymerase activity Endonuclease activity |
Issue Date | 2017 |
Publisher | Society for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org |
Citation | Journal of General Virology, 2017, v. 98 n. 3, p. 364-373 How to Cite? |
Abstract | Substitutions in the PA N-terminus (PAN) of influenza A viruses are associated with viral pathogenicity. During our previous study, which identified PAN-V63I and -A37S/I61T/V63I/V100A substitutions as virulence determinants, we observed a severe decrease in virus growth and transcription/replication capacity posed by PAN-A37S/V100A substitution. To further delineate the significance of substitutions at these positions, we generated mutant H7N7 viruses bearing the substitutions PAN-A37S, -A37S/I61T, -A37S/V63I, -V100A, -I61T/V100A and -V63I/V100A by reverse genetics. Our results showed that all mutant viruses except PAN-V100A showed a significantly reduced growth capability in infected cells. At the same time, the PAN-A37S, -A37S/I61T and -A37S/V63I mutant viruses displayed decreased viral transcription and replication by diminishing virus RNA synthesis activity. Biochemical assays indicated that the substitutions PAN-A37S, -A37S/I61T and -A37S/V63I suppressed the polymerase and endonuclease activities when compared with those of the wild-type. Together, our results demonstrated that the PAN-A37S, -A37S/I61T and -A37S/V63I substitutions contributed to a decreased pathogenicity of avian H7N7 influenza A virus. |
Persistent Identifier | http://hdl.handle.net/10722/243182 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 0.990 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Hu, M | - |
dc.contributor.author | Yuan, S | - |
dc.contributor.author | Ye, Z | - |
dc.contributor.author | Singh, K | - |
dc.contributor.author | Li, C | - |
dc.contributor.author | Shuai, H | - |
dc.contributor.author | Ng, F | - |
dc.contributor.author | Chow, BKC | - |
dc.contributor.author | Chu, H | - |
dc.contributor.author | Zheng, B | - |
dc.date.accessioned | 2017-08-25T02:51:15Z | - |
dc.date.available | 2017-08-25T02:51:15Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Journal of General Virology, 2017, v. 98 n. 3, p. 364-373 | - |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.uri | http://hdl.handle.net/10722/243182 | - |
dc.description.abstract | Substitutions in the PA N-terminus (PAN) of influenza A viruses are associated with viral pathogenicity. During our previous study, which identified PAN-V63I and -A37S/I61T/V63I/V100A substitutions as virulence determinants, we observed a severe decrease in virus growth and transcription/replication capacity posed by PAN-A37S/V100A substitution. To further delineate the significance of substitutions at these positions, we generated mutant H7N7 viruses bearing the substitutions PAN-A37S, -A37S/I61T, -A37S/V63I, -V100A, -I61T/V100A and -V63I/V100A by reverse genetics. Our results showed that all mutant viruses except PAN-V100A showed a significantly reduced growth capability in infected cells. At the same time, the PAN-A37S, -A37S/I61T and -A37S/V63I mutant viruses displayed decreased viral transcription and replication by diminishing virus RNA synthesis activity. Biochemical assays indicated that the substitutions PAN-A37S, -A37S/I61T and -A37S/V63I suppressed the polymerase and endonuclease activities when compared with those of the wild-type. Together, our results demonstrated that the PAN-A37S, -A37S/I61T and -A37S/V63I substitutions contributed to a decreased pathogenicity of avian H7N7 influenza A virus. | - |
dc.language | eng | - |
dc.publisher | Society for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org | - |
dc.relation.ispartof | Journal of General Virology | - |
dc.subject | Influenza A virus | - |
dc.subject | PAN-A37S | - |
dc.subject | PAN-A37S/I61T | - |
dc.subject | PAN-A37S/V63I | - |
dc.subject | Polymerase activity | - |
dc.subject | Endonuclease activity | - |
dc.title | PAN substitutions A37S, A37S/I61T and A37S/V63I attenuate the replication of H7N7 influenza A virus by impairing the polymerase and endonuclease activities | - |
dc.type | Article | - |
dc.identifier.email | Hu, M: meng16hu@hku.hk | - |
dc.identifier.email | Yuan, S: yuansf@hku.hk | - |
dc.identifier.email | Ye, Z: zwye@hku.hk | - |
dc.identifier.email | Li, C: licun@hku.hk | - |
dc.identifier.email | Ng, F: fng@hku.hk | - |
dc.identifier.email | Chow, BKC: bkcc@hku.hk | - |
dc.identifier.email | Chu, H: hinchu@hku.hk | - |
dc.identifier.email | Zheng, B: bzheng@hkucc.hku.hk | - |
dc.identifier.authority | Chow, BKC=rp00681 | - |
dc.identifier.authority | Chu, H=rp02125 | - |
dc.identifier.authority | Zheng, B=rp00353 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1099/jgv.0.000717 | - |
dc.identifier.pmid | 28113045 | - |
dc.identifier.scopus | eid_2-s2.0-85017011014 | - |
dc.identifier.hkuros | 273769 | - |
dc.identifier.volume | 98 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 364 | - |
dc.identifier.epage | 373 | - |
dc.identifier.isi | WOS:000399235600008 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0022-1317 | - |