File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification of serum miR-139-3p as a non-invasive biomarker for colorectal cancer

TitleIdentification of serum miR-139-3p as a non-invasive biomarker for colorectal cancer
Authors
KeywordsBiomarker
CRC
MiR-139-3p
MiR-622
MiRNA
Issue Date2017
PublisherImpact Journals LLC. The Journal's web site is located at http://www.impactjournals.com/oncotarget/index.html
Citation
Oncotarget, 2017, v. 8 n. 16, p. 27393-27400 How to Cite?
AbstractAberrant levels of circulating microRNAs are potential biomarkers for the early detection of colorectal cancer. The aim of this study was to study miR-139-3p and miR-622 in serum as a non-invasive biomarker for colorectal cancer diagnosis. We applied quantitative polymerase chain reaction to determine the levels of miR-139-3p and miR-622 in 42 pairs of tumor and adjacent non-tumor tissues, and in serum samples of 117 patients and 90 control subjects. Our results showed that miR-139-3p was silenced whereas miR-622 was overexpressed in colorectal cancer. Similarly, serum miR-139-3p level was significantly lower in colorectal cancer patients than in control subjects whereas miR-622 was more frequently detectable in patients. ROC analysis showed that AUC of miR-139-3p was 0.9935, with a sensitivity of 96.6% and specificity of 97.8%. Serum miR-139-3p level showed high sensitivity and specificity for both early and late stage CRCs and proximal and distal CRCs. Detectable serum miR-622 showed a sensitivity of 87.5% and specificity of 63.5% for discriminating CRC patients, but the sensitivity dropped for late stage patients (72.7%). We also included analyses of the blood CEA level for comparing the diagnostic performance of these blood-based biomarkers. The median level in CRC patients (3.6 ng/ml) was significantly higher than that in control (1.8 ng/ml). The AUC value of CEA in diagnosing CRC patients was 0.7515. CEA showed a positive correlation with tumor stage and age of patients and its level was higher in male. Collectively, serum miR-139-3p has strong potential as a promising non-invasive biomarker in colorectal cancer detection.
Persistent Identifierhttp://hdl.handle.net/10722/243158
ISSN
2016 Impact Factor: 5.168
2023 SCImago Journal Rankings: 0.789
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNg, L-
dc.contributor.authorWan, TMH-
dc.contributor.authorMan, HW-
dc.contributor.authorChow, KM-
dc.contributor.authorLyer, D-
dc.contributor.authorChen, G-
dc.contributor.authorYau, TCC-
dc.contributor.authorLo, OSH-
dc.contributor.authorFoo, CC-
dc.contributor.authorPoon, TCJ-
dc.contributor.authorLeung, WK-
dc.contributor.authorPang, RWC-
dc.contributor.authorLaw, WL-
dc.date.accessioned2017-08-25T02:50:51Z-
dc.date.available2017-08-25T02:50:51Z-
dc.date.issued2017-
dc.identifier.citationOncotarget, 2017, v. 8 n. 16, p. 27393-27400-
dc.identifier.issn1949-2553-
dc.identifier.urihttp://hdl.handle.net/10722/243158-
dc.description.abstractAberrant levels of circulating microRNAs are potential biomarkers for the early detection of colorectal cancer. The aim of this study was to study miR-139-3p and miR-622 in serum as a non-invasive biomarker for colorectal cancer diagnosis. We applied quantitative polymerase chain reaction to determine the levels of miR-139-3p and miR-622 in 42 pairs of tumor and adjacent non-tumor tissues, and in serum samples of 117 patients and 90 control subjects. Our results showed that miR-139-3p was silenced whereas miR-622 was overexpressed in colorectal cancer. Similarly, serum miR-139-3p level was significantly lower in colorectal cancer patients than in control subjects whereas miR-622 was more frequently detectable in patients. ROC analysis showed that AUC of miR-139-3p was 0.9935, with a sensitivity of 96.6% and specificity of 97.8%. Serum miR-139-3p level showed high sensitivity and specificity for both early and late stage CRCs and proximal and distal CRCs. Detectable serum miR-622 showed a sensitivity of 87.5% and specificity of 63.5% for discriminating CRC patients, but the sensitivity dropped for late stage patients (72.7%). We also included analyses of the blood CEA level for comparing the diagnostic performance of these blood-based biomarkers. The median level in CRC patients (3.6 ng/ml) was significantly higher than that in control (1.8 ng/ml). The AUC value of CEA in diagnosing CRC patients was 0.7515. CEA showed a positive correlation with tumor stage and age of patients and its level was higher in male. Collectively, serum miR-139-3p has strong potential as a promising non-invasive biomarker in colorectal cancer detection.-
dc.languageeng-
dc.publisherImpact Journals LLC. The Journal's web site is located at http://www.impactjournals.com/oncotarget/index.html-
dc.relation.ispartofOncotarget-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectBiomarker-
dc.subjectCRC-
dc.subjectMiR-139-3p-
dc.subjectMiR-622-
dc.subjectMiRNA-
dc.titleIdentification of serum miR-139-3p as a non-invasive biomarker for colorectal cancer-
dc.typeArticle-
dc.identifier.emailNg, L: luing@hku.hk-
dc.identifier.emailWan, TMH: tmhwan@hku.hk-
dc.identifier.emailMan, HW: johnnyb@hku.hk-
dc.identifier.emailChow, KM: chowakm@hku.hk-
dc.identifier.emailChen, G: guanghua@hku.hk-
dc.identifier.emailYau, TCC: tyaucc@hku.hk-
dc.identifier.emailLo, OSH: oswens@hku.hk-
dc.identifier.emailFoo, CC: ccfoo@hku.hk-
dc.identifier.emailPoon, TCJ: tcjensen@hku.hk-
dc.identifier.emailLeung, WK: hku75407@hku.hk-
dc.identifier.emailPang, RWC: robertap@hku.hk-
dc.identifier.emailLaw, WL: lawwl@hkucc.hku.hk-
dc.identifier.authorityNg, L=rp02207-
dc.identifier.authorityYau, TCC=rp01466-
dc.identifier.authorityFoo, CC=rp01899-
dc.identifier.authorityPoon, TCJ=rp01603-
dc.identifier.authorityLeung, WK=rp01479-
dc.identifier.authorityPang, RWC=rp00274-
dc.identifier.authorityLaw, WL=rp00436-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.18632/oncotarget.16171-
dc.identifier.scopuseid_2-s2.0-85017509589-
dc.identifier.hkuros273711-
dc.identifier.volume8-
dc.identifier.issue16-
dc.identifier.spage27393-
dc.identifier.epage27400-
dc.identifier.isiWOS:000399819700127-
dc.publisher.placeUnited States-
dc.identifier.issnl1949-2553-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats