File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1039/C5SC02467F
- Scopus: eid_2-s2.0-84946565890
- WOS: WOS:000365225300049
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Design, preparation, and selection of DNA-encoded dynamic libraries
Title | Design, preparation, and selection of DNA-encoded dynamic libraries |
---|---|
Authors | |
Issue Date | 2015 |
Publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp |
Citation | Chemical Science, 2015, v. 6 n. 12, p. 7097-7104 How to Cite? |
Abstract | We report a method for the preparation and selection of DNA-encoded dynamic libraries (DEDLs). The library is composed of two sets of DNA-linked small molecules that are under dynamic exchange through DNA hybridization. Addition of the protein target shifted the equilibrium, favouring the assembly of high affinity bivalent binders. Notably, we introduced a novel locking mechanism to stop the dynamic exchange and “freeze” the equilibrium, thereby enabling downstream hit isolation and decoding by PCR amplification and DNA sequencing. Our DEDL approach has circumvented the limitation of library size and realized the analysis and selection of large dynamic libraries. In addition, this method also eliminates the requirement for modified and immobilized target proteins. |
Persistent Identifier | http://hdl.handle.net/10722/243010 |
ISSN | 2023 Impact Factor: 7.6 2023 SCImago Journal Rankings: 2.333 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, G | - |
dc.contributor.author | Zheng, W | - |
dc.contributor.author | Chen, Z | - |
dc.contributor.author | Zhou, Y | - |
dc.contributor.author | Liu, Y | - |
dc.contributor.author | Yang, J | - |
dc.contributor.author | Huang, Y | - |
dc.contributor.author | Li, X | - |
dc.date.accessioned | 2017-08-25T02:48:41Z | - |
dc.date.available | 2017-08-25T02:48:41Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Chemical Science, 2015, v. 6 n. 12, p. 7097-7104 | - |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.uri | http://hdl.handle.net/10722/243010 | - |
dc.description.abstract | We report a method for the preparation and selection of DNA-encoded dynamic libraries (DEDLs). The library is composed of two sets of DNA-linked small molecules that are under dynamic exchange through DNA hybridization. Addition of the protein target shifted the equilibrium, favouring the assembly of high affinity bivalent binders. Notably, we introduced a novel locking mechanism to stop the dynamic exchange and “freeze” the equilibrium, thereby enabling downstream hit isolation and decoding by PCR amplification and DNA sequencing. Our DEDL approach has circumvented the limitation of library size and realized the analysis and selection of large dynamic libraries. In addition, this method also eliminates the requirement for modified and immobilized target proteins. | - |
dc.language | eng | - |
dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp | - |
dc.relation.ispartof | Chemical Science | - |
dc.title | Design, preparation, and selection of DNA-encoded dynamic libraries | - |
dc.type | Article | - |
dc.identifier.email | Zhou, Y: yuchow@hku.hk | - |
dc.identifier.email | Li, X: xiaoyuli@hku.hk | - |
dc.identifier.authority | Li, X=rp02080 | - |
dc.identifier.doi | 10.1039/C5SC02467F | - |
dc.identifier.pmcid | PMC5510007 | - |
dc.identifier.scopus | eid_2-s2.0-84946565890 | - |
dc.identifier.hkuros | 274755 | - |
dc.identifier.volume | 6 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 7097 | - |
dc.identifier.epage | 7104 | - |
dc.identifier.isi | WOS:000365225300049 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2041-6520 | - |