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- Publisher Website: 10.1016/j.humpath.2017.01.014
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Article: MiR-498 in esophageal squamous cell carcinoma: clinicopathological impacts and functional interactions
Title | MiR-498 in esophageal squamous cell carcinoma: clinicopathological impacts and functional interactions |
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Authors | |
Keywords | Esophageal Grade miR-498 Squamous cell carcinoma Survival |
Issue Date | 2017 |
Publisher | WB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/humpath |
Citation | Human Pathology, 2017, v. 62, p. 141-151 How to Cite? |
Abstract | MicroRNA-498 plays a crucial role in progression of many carcinomas. The signaling pathways by which miR-498 modulates carcinogenesis are still unknown. Also, miR-498–associated molecular pathogenesis has never been studied in esophageal squamous cell carcinoma (ESCC). Herein, we aimed to examine the expression and functional roles of miR-498 in ESCC as well as its influences on the clinicopathological features in patients with ESCC. Expression of miR-498 was investigated in 93 ESCC tissues and 5 ESCC cell lines using quantitative real-time polymerase chain reaction. In vitro effects of miR-498 on cellular process were studied followed by overexpression of miR-498. Western blot and immunofluorescence techniques were used to identify the interacting targets for miR-498 in ESCC. miR-498 expression was significantly reduced in ESCC when compared with the nonneoplastic esophageal tissues (P < .05). Patients with low miR-498 expression showed different histological grading of cancer and survival rates when compared with the patients with high miR-498 expression. Overexpression of miR-498 in ESCC cell lines induced remarkable reductions of cell proliferation, barrier penetration, and colony formation when compared with control and wild-type counterparts. Also, miR-498 activated the FOXO1/KLF6 transcriptional axis in ESCC. In addition, miR-498 overexpression increased p21 protein expression and led to reduced cancer cell growth. To conclude, reduced expression of miR-498 in ESCC and in vitro analysis have confirmed the tumor suppressor properties of miR-498 by modulating the FOXO1/KLF6 signaling pathway. The changes in miR-498 expression may have impacts on the clinical pathological parameters of ESCC as well as in the management of the patients with ESCC. |
Persistent Identifier | http://hdl.handle.net/10722/242279 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.936 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Islam, F | - |
dc.contributor.author | Gopalan, V | - |
dc.contributor.author | Law, SYK | - |
dc.contributor.author | Tang, JCO | - |
dc.contributor.author | Chan, KW | - |
dc.contributor.author | Lam, AKY | - |
dc.date.accessioned | 2017-07-24T01:37:40Z | - |
dc.date.available | 2017-07-24T01:37:40Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Human Pathology, 2017, v. 62, p. 141-151 | - |
dc.identifier.issn | 0046-8177 | - |
dc.identifier.uri | http://hdl.handle.net/10722/242279 | - |
dc.description.abstract | MicroRNA-498 plays a crucial role in progression of many carcinomas. The signaling pathways by which miR-498 modulates carcinogenesis are still unknown. Also, miR-498–associated molecular pathogenesis has never been studied in esophageal squamous cell carcinoma (ESCC). Herein, we aimed to examine the expression and functional roles of miR-498 in ESCC as well as its influences on the clinicopathological features in patients with ESCC. Expression of miR-498 was investigated in 93 ESCC tissues and 5 ESCC cell lines using quantitative real-time polymerase chain reaction. In vitro effects of miR-498 on cellular process were studied followed by overexpression of miR-498. Western blot and immunofluorescence techniques were used to identify the interacting targets for miR-498 in ESCC. miR-498 expression was significantly reduced in ESCC when compared with the nonneoplastic esophageal tissues (P < .05). Patients with low miR-498 expression showed different histological grading of cancer and survival rates when compared with the patients with high miR-498 expression. Overexpression of miR-498 in ESCC cell lines induced remarkable reductions of cell proliferation, barrier penetration, and colony formation when compared with control and wild-type counterparts. Also, miR-498 activated the FOXO1/KLF6 transcriptional axis in ESCC. In addition, miR-498 overexpression increased p21 protein expression and led to reduced cancer cell growth. To conclude, reduced expression of miR-498 in ESCC and in vitro analysis have confirmed the tumor suppressor properties of miR-498 by modulating the FOXO1/KLF6 signaling pathway. The changes in miR-498 expression may have impacts on the clinical pathological parameters of ESCC as well as in the management of the patients with ESCC. | - |
dc.language | eng | - |
dc.publisher | WB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/humpath | - |
dc.relation.ispartof | Human Pathology | - |
dc.subject | Esophageal | - |
dc.subject | Grade | - |
dc.subject | miR-498 | - |
dc.subject | Squamous cell carcinoma | - |
dc.subject | Survival | - |
dc.title | MiR-498 in esophageal squamous cell carcinoma: clinicopathological impacts and functional interactions | - |
dc.type | Article | - |
dc.identifier.email | Law, SYK: slaw@hku.hk | - |
dc.identifier.email | Tang, JCO: cotang@hkucc.hku.hk | - |
dc.identifier.email | Chan, KW: hrmtckw@hku.hk | - |
dc.identifier.email | Lam, AKY: akylam@hkucc.hku.hk | - |
dc.identifier.authority | Law, SYK=rp00437 | - |
dc.identifier.authority | Chan, KW=rp00330 | - |
dc.identifier.doi | 10.1016/j.humpath.2017.01.014 | - |
dc.identifier.scopus | eid_2-s2.0-85015688647 | - |
dc.identifier.hkuros | 272944 | - |
dc.identifier.volume | 62 | - |
dc.identifier.spage | 141 | - |
dc.identifier.epage | 151 | - |
dc.identifier.isi | WOS:000400230800019 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0046-8177 | - |