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Article: Arabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition

TitleArabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition
Authors
KeywordsAcyl-CoA-binding protein
Arabidopsis
Companion cell
OPDA
Phloem exudates
Sieve elements
Issue Date2016
Citation
Plant Molecular Biology, 2016, v. 92 n. 6, p. 717-730 How to Cite?
AbstractArabidopsis thaliana ACYL-COA-BINDING PROTEIN6 (AtACBP6) encodes a cytosolic 10-kDa AtACBP. It confers freezing tolerance in transgenic Arabidopsis, possibly by its interaction with lipids as indicated by the binding of acyl-CoA esters and phosphatidylcholine to recombinant AtACBP6. Herein, transgenic Arabidopsis transformed with an AtACBP6 promoter-driven β-glucuronidase (GUS) construct exhibited strong GUS activity in the vascular tissues. Immunoelectron microscopy using anti-AtACBP6 antibodies showed AtACBP6 localization in the phloem especially in the companion cells and sieve elements. Also, the presence of gold grains in the plasmodesmata indicated its potential role in systemic trafficking. The AtACBP6 protein, but not its mRNA, was found in phloem exudate of wild-type Arabidopsis. Fatty acid profiling using gas chromatography-mass spectrometry revealed an increase in the jasmonic acid (JA) precursor, 12-oxo-cis,cis-10,15-phytodienoic acid (cis-OPDA), and a reduction in JA and/or its derivatives in acbp6 phloem exudates in comparison to the wild type. Quantitative real-time PCR showed down-regulation of COMATOSE (CTS) in acbp6 rosettes suggesting that AtACBP6 affects CTS function. AtACBP6 appeared to affect the content of JA and/or its derivatives in the sieve tubes, which is consistent with its role in pathogen-defense and in its wound-inducibility of AtACBP6pro::GUS. Taken together, our results suggest the involvement of AtACBP6 in JA-biosynthesis in Arabidopsis phloem tissues.
Persistent Identifierhttp://hdl.handle.net/10722/238765
ISSN
2020 Impact Factor: 4.076
2020 SCImago Journal Rankings: 1.448
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYE, Z-
dc.contributor.authorLung, SC-
dc.contributor.authorHU, T-
dc.contributor.authorChen, Q-
dc.contributor.authorSUEN, YL-
dc.contributor.authorWang, M-
dc.contributor.authorHoffmann-Benning, S-
dc.contributor.authorYeung, E-
dc.contributor.authorChye, ML-
dc.date.accessioned2017-02-20T01:25:48Z-
dc.date.available2017-02-20T01:25:48Z-
dc.date.issued2016-
dc.identifier.citationPlant Molecular Biology, 2016, v. 92 n. 6, p. 717-730-
dc.identifier.issn0167-4412-
dc.identifier.urihttp://hdl.handle.net/10722/238765-
dc.description.abstractArabidopsis thaliana ACYL-COA-BINDING PROTEIN6 (AtACBP6) encodes a cytosolic 10-kDa AtACBP. It confers freezing tolerance in transgenic Arabidopsis, possibly by its interaction with lipids as indicated by the binding of acyl-CoA esters and phosphatidylcholine to recombinant AtACBP6. Herein, transgenic Arabidopsis transformed with an AtACBP6 promoter-driven β-glucuronidase (GUS) construct exhibited strong GUS activity in the vascular tissues. Immunoelectron microscopy using anti-AtACBP6 antibodies showed AtACBP6 localization in the phloem especially in the companion cells and sieve elements. Also, the presence of gold grains in the plasmodesmata indicated its potential role in systemic trafficking. The AtACBP6 protein, but not its mRNA, was found in phloem exudate of wild-type Arabidopsis. Fatty acid profiling using gas chromatography-mass spectrometry revealed an increase in the jasmonic acid (JA) precursor, 12-oxo-cis,cis-10,15-phytodienoic acid (cis-OPDA), and a reduction in JA and/or its derivatives in acbp6 phloem exudates in comparison to the wild type. Quantitative real-time PCR showed down-regulation of COMATOSE (CTS) in acbp6 rosettes suggesting that AtACBP6 affects CTS function. AtACBP6 appeared to affect the content of JA and/or its derivatives in the sieve tubes, which is consistent with its role in pathogen-defense and in its wound-inducibility of AtACBP6pro::GUS. Taken together, our results suggest the involvement of AtACBP6 in JA-biosynthesis in Arabidopsis phloem tissues.-
dc.languageeng-
dc.relation.ispartofPlant Molecular Biology-
dc.subjectAcyl-CoA-binding protein-
dc.subjectArabidopsis-
dc.subjectCompanion cell-
dc.subjectOPDA-
dc.subjectPhloem exudates-
dc.subjectSieve elements-
dc.titleArabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition-
dc.typeArticle-
dc.identifier.emailLung, SC: h0024385@graduate.hku.hk-
dc.identifier.emailWang, M: mfwang@hku.hk-
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hk-
dc.identifier.authorityWang, M=rp00800-
dc.identifier.authorityChye, ML=rp00687-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11103-016-0541-0-
dc.identifier.pmid27645136-
dc.identifier.scopuseid_2-s2.0-84988370299-
dc.identifier.hkuros271188-
dc.identifier.volume92-
dc.identifier.issue6-
dc.identifier.spage717-
dc.identifier.epage730-
dc.identifier.eissn1573-5028-
dc.identifier.isiWOS:000388697000007-
dc.identifier.issnl0167-4412-

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