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Article: Exome-chip association analysis reveals an Asian-specific missense variant in PAX4 associated with type 2 diabetes in Chinese individuals

TitleExome-chip association analysis reveals an Asian-specific missense variant in PAX4 associated with type 2 diabetes in Chinese individuals
Authors
KeywordsAsian-specific
Exome-chip association analysis
Type 2 diabetes
PAX4
Issue Date2017
Citation
Diabetologia, 2017, v. 60, n. 1, p. 107-115 How to Cite?
Abstract© 2016, Springer-Verlag Berlin Heidelberg.Aims/hypothesis: Genome-wide association studies (GWASs) have identified many common type 2 diabetes-associated variants, mostly at the intronic or intergenic regions. Recent advancements of exome-array genotyping platforms have opened up a novel means for detecting the associations of low-frequency or rare coding variants with type 2 diabetes. We conducted an exomechip association analysis to identify additional type 2 diabetes susceptibility variants in the Chinese population. Methods: An exome-chip association study was conducted by genotyping 5640 Chinese individuals from Hong Kong, using a custom designed exome array, the Asian Exomechip. Single variant association analysis was conducted on 77,468 single nucleotide polymorphisms (SNPs). Fifteen SNPs were subsequently genotyped for replication analysis in an independent Chinese cohort comprising 12,362 individuals from Guangzhou. A combined analysis involving 7189 cases and 10,813 controls was performed. Results: In the discovery stage, an Asian-specific coding variant rs2233580 (p.Arg192His) in PAX4, and two variants at the known loci, CDKN2B-AS1 and KCNQ1, were significantly associated with type 2 diabetes with exome-wide significance (pdiscovery < 6.45 × 10−7). The risk allele (T) of PAX4 rs2233580 was associated with a younger age at diabetes diagnosis. This variant was replicated in an independent cohort and demonstrated a stronger association that reached genome-wide significance (pmeta-analysis [pmeta] = 3.74 × 10−15) in the combined analysis. Conclusions/interpretation: We identified the association of a PAX4 Asian-specific missense variant rs2233580 with type 2 diabetes in an exome-chip association analysis, supporting the involvement of PAX4 in the pathogenesis of type 2 diabetes. Our findings suggest PAX4 is a possible effector gene of the 7q32 locus, previously identified from GWAS in Asians.
Persistent Identifierhttp://hdl.handle.net/10722/238162
ISSN
2023 Impact Factor: 8.4
2023 SCImago Journal Rankings: 3.355
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheung, Chloe Y Y-
dc.contributor.authorTang, Clara S.-
dc.contributor.authorXu, Aimin-
dc.contributor.authorLee, Chi Ho-
dc.contributor.authorAu, Ka Wing-
dc.contributor.authorXu, Lin-
dc.contributor.authorFong, Carol H Y-
dc.contributor.authorKwok, Kelvin H M-
dc.contributor.authorChow, Wing Sun-
dc.contributor.authorWoo, Yu Cho-
dc.contributor.authorYuen, Michele M A-
dc.contributor.authorHai, Jo Jo S H-
dc.contributor.authorJin, Ya Li-
dc.contributor.authorCheung, Bernard M Y-
dc.contributor.authorTan, Kathryn C B-
dc.contributor.authorCherny, Stacey S.-
dc.contributor.authorZhu, Feng-
dc.contributor.authorZhu, Tong-
dc.contributor.authorThomas, G. Neil-
dc.contributor.authorCheng, Kar Keung-
dc.contributor.authorJiang, Chao Qiang-
dc.contributor.authorLam, Tai Hing-
dc.contributor.authorTse, Hung Fat-
dc.contributor.authorSham, Pak Chung-
dc.contributor.authorLam, Karen S L-
dc.date.accessioned2017-02-03T02:13:14Z-
dc.date.available2017-02-03T02:13:14Z-
dc.date.issued2017-
dc.identifier.citationDiabetologia, 2017, v. 60, n. 1, p. 107-115-
dc.identifier.issn0012-186X-
dc.identifier.urihttp://hdl.handle.net/10722/238162-
dc.description.abstract© 2016, Springer-Verlag Berlin Heidelberg.Aims/hypothesis: Genome-wide association studies (GWASs) have identified many common type 2 diabetes-associated variants, mostly at the intronic or intergenic regions. Recent advancements of exome-array genotyping platforms have opened up a novel means for detecting the associations of low-frequency or rare coding variants with type 2 diabetes. We conducted an exomechip association analysis to identify additional type 2 diabetes susceptibility variants in the Chinese population. Methods: An exome-chip association study was conducted by genotyping 5640 Chinese individuals from Hong Kong, using a custom designed exome array, the Asian Exomechip. Single variant association analysis was conducted on 77,468 single nucleotide polymorphisms (SNPs). Fifteen SNPs were subsequently genotyped for replication analysis in an independent Chinese cohort comprising 12,362 individuals from Guangzhou. A combined analysis involving 7189 cases and 10,813 controls was performed. Results: In the discovery stage, an Asian-specific coding variant rs2233580 (p.Arg192His) in PAX4, and two variants at the known loci, CDKN2B-AS1 and KCNQ1, were significantly associated with type 2 diabetes with exome-wide significance (pdiscovery < 6.45 × 10−7). The risk allele (T) of PAX4 rs2233580 was associated with a younger age at diabetes diagnosis. This variant was replicated in an independent cohort and demonstrated a stronger association that reached genome-wide significance (pmeta-analysis [pmeta] = 3.74 × 10−15) in the combined analysis. Conclusions/interpretation: We identified the association of a PAX4 Asian-specific missense variant rs2233580 with type 2 diabetes in an exome-chip association analysis, supporting the involvement of PAX4 in the pathogenesis of type 2 diabetes. Our findings suggest PAX4 is a possible effector gene of the 7q32 locus, previously identified from GWAS in Asians.-
dc.languageeng-
dc.relation.ispartofDiabetologia-
dc.subjectAsian-specific-
dc.subjectExome-chip association analysis-
dc.subjectType 2 diabetes-
dc.subjectPAX4-
dc.titleExome-chip association analysis reveals an Asian-specific missense variant in PAX4 associated with type 2 diabetes in Chinese individuals-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1007/s00125-016-4132-z-
dc.identifier.scopuseid_2-s2.0-84991396072-
dc.identifier.hkuros272186-
dc.identifier.volume60-
dc.identifier.issue1-
dc.identifier.spage107-
dc.identifier.epage115-
dc.identifier.eissn1432-0428-
dc.identifier.isiWOS:000389634000014-
dc.identifier.issnl0012-186X-

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