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- Publisher Website: 10.1111/cpr.12172
- Scopus: eid_2-s2.0-84924000022
- PMID: 25643922
- WOS: WOS:000350978400011
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Article: Stimulation of EphB2/ephrin-B1 signalling by tumour necrosis factor alpha in human dental pulp stem cells
Title | Stimulation of EphB2/ephrin-B1 signalling by tumour necrosis factor alpha in human dental pulp stem cells |
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Authors | |
Issue Date | 2015 |
Citation | Cell Proliferation, 2015, v. 48, n. 2, p. 231-238 How to Cite? |
Abstract | © 2015 John Wiley & Sons Ltd.Objectives: The aim of this study was to investigate whether in vitro stimulation of dental pulp stem cells (DPSCs) by tumour necrosis factor alpha (TNF-α) would induce secretion of EphB2/ephrin-B1 signalling.Materials and methods: Dental pulp stem cells isolated from human dental pulp were treated with TNF-α (5-100 ng/ml) over 2-48 h. EphB2/ephrin-B1 mRNA and protein levels were measured by real-time polymerase chain reaction (RT-PCR) and western blot analysis respectively. Additionally, DPSCs were pre-incubated with TNF-α receptor neutralizing antibodies or infected with nuclear factor-kappa B (NF-K{green}B) inhibitor, p38 MAPK inhibitor, Jun N-terminal kinase (JNK) inhibitor and MEK inhibitor before TNF-α treatment. Results were analysed by one-way ANOVA. Results: Tumour necrosis factor alpha increased EphB2 mRNA expression in DPSCs at concentrations up to 20 ng/ml and ephrin-B1 at concentrations up to 40 ng/ml (P < 0.05). Its mRNA expression reached maximum at 24 h when treated with TNF-α at 20 ng/ml (P < 0.05). EphB2/ephrin-B1 protein expression levels were high at 16 and 24 h as shown by western blotting. Neutralizing antibodies for TNFR1/2 receptors down-regulated EphB2/ephrin-B1 mRNA expression (P < 0.05) and ephrin-B1 protein expression, but not EphB2 protein expression. JNK-inhibitor inhibited EphB2 mRNA expression only (P < 0.05).Conclusions: EphB2/ephrin-B1 were invoked in DPSCs with TNF-α treatment via the JNK-dependent pathway, but not NF-K{green}B, p38 MAPK or MEK signalling. |
Persistent Identifier | http://hdl.handle.net/10722/236238 |
ISSN | 2023 Impact Factor: 5.9 2023 SCImago Journal Rankings: 1.951 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhu, Lifang | - |
dc.contributor.author | Dissanayaka, Waruna Lakmal | - |
dc.contributor.author | Green, David William | - |
dc.contributor.author | Zhang, Chengfei | - |
dc.date.accessioned | 2016-11-11T07:43:19Z | - |
dc.date.available | 2016-11-11T07:43:19Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Cell Proliferation, 2015, v. 48, n. 2, p. 231-238 | - |
dc.identifier.issn | 0960-7722 | - |
dc.identifier.uri | http://hdl.handle.net/10722/236238 | - |
dc.description.abstract | © 2015 John Wiley & Sons Ltd.Objectives: The aim of this study was to investigate whether in vitro stimulation of dental pulp stem cells (DPSCs) by tumour necrosis factor alpha (TNF-α) would induce secretion of EphB2/ephrin-B1 signalling.Materials and methods: Dental pulp stem cells isolated from human dental pulp were treated with TNF-α (5-100 ng/ml) over 2-48 h. EphB2/ephrin-B1 mRNA and protein levels were measured by real-time polymerase chain reaction (RT-PCR) and western blot analysis respectively. Additionally, DPSCs were pre-incubated with TNF-α receptor neutralizing antibodies or infected with nuclear factor-kappa B (NF-K{green}B) inhibitor, p38 MAPK inhibitor, Jun N-terminal kinase (JNK) inhibitor and MEK inhibitor before TNF-α treatment. Results were analysed by one-way ANOVA. Results: Tumour necrosis factor alpha increased EphB2 mRNA expression in DPSCs at concentrations up to 20 ng/ml and ephrin-B1 at concentrations up to 40 ng/ml (P < 0.05). Its mRNA expression reached maximum at 24 h when treated with TNF-α at 20 ng/ml (P < 0.05). EphB2/ephrin-B1 protein expression levels were high at 16 and 24 h as shown by western blotting. Neutralizing antibodies for TNFR1/2 receptors down-regulated EphB2/ephrin-B1 mRNA expression (P < 0.05) and ephrin-B1 protein expression, but not EphB2 protein expression. JNK-inhibitor inhibited EphB2 mRNA expression only (P < 0.05).Conclusions: EphB2/ephrin-B1 were invoked in DPSCs with TNF-α treatment via the JNK-dependent pathway, but not NF-K{green}B, p38 MAPK or MEK signalling. | - |
dc.language | eng | - |
dc.relation.ispartof | Cell Proliferation | - |
dc.title | Stimulation of EphB2/ephrin-B1 signalling by tumour necrosis factor alpha in human dental pulp stem cells | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1111/cpr.12172 | - |
dc.identifier.pmid | 25643922 | - |
dc.identifier.scopus | eid_2-s2.0-84924000022 | - |
dc.identifier.hkuros | 242672 | - |
dc.identifier.volume | 48 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 231 | - |
dc.identifier.epage | 238 | - |
dc.identifier.eissn | 1365-2184 | - |
dc.identifier.isi | WOS:000350978400011 | - |
dc.identifier.issnl | 0960-7722 | - |