File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1111/iej.12354
- Scopus: eid_2-s2.0-84929131880
- WOS: WOS:000354294300013
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or Er: YAG laser in vitro
Title | Antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or Er: YAG laser in vitro |
---|---|
Authors | |
Keywords | Continuous chelation Confocal microscopy Biofilm Enterococcus faecalis Soft chelation Sodium hypochlorite |
Issue Date | 2015 |
Citation | International Endodontic Journal, 2015, v. 48, n. 6, p. 602-610 How to Cite? |
Abstract | © 2014 International Endodontic Journal.Aim: To investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of Enterococcus faecalis in vitro. Methodology: Root canals in 280 single-rooted teeth were instrumented using a rotary Ni-Ti system. Biofilms of E. faecalis were generated based on a previously established protocol. Samples were randomly divided into three experimental (n = 80) and one control (n = 40) group based on the irrigation protocol employed: group 1 (NaOCl + Etidronic acid), 1 : 1 mixture of 6% NaOCl and 18% etidronic acid; group 2 (NaOCl-EDTA), 3% NaOCl followed by 17% EDTA; group 3 (NaOCl-EDTA-NaOCl), 3% NaOCl followed by 17% EDTA and a final flush of 3% NaOCl. Saline served as the control. Samples were further divided into four subgroups (n = 20) based on the activation method: subgroup A, no activation; subgroup B, ultrasonic activation; group C, diode laser; group D, Er:YAG laser. Confocal laser scanning microscopy was used to assess bacterial viability in situ. Root dentine powder was obtained for determining the colony-forming units (CFU mL-1). Data were analysed by appropriate statistical analyses with P = 0.05. Results: All experimental irrigation protocols caused complete destruction of the biofilm in the root canal lumen. Within the dentinal tubules, all groups had a significantly higher percentage of dead bacteria than the saline control (P < 0.05). There was no significant difference between NaOCl + etidronic acid and NaOCl-EDTA-NaOCl (P > 0.05), whereas both groups brought about more bacterial reduction than NaOCl-EDTA (P < 0.05). There was no significant difference between diode laser and Er:YAG laser in any of the groups (P > 0.05). Both diode and Er:YAG laser were more effective than ultrasonic activation and conventional syringe irrigation in reducing E. fecalis biofilms (P < 0.05). Conclusions: The use of NaOCl after or in combination with a chelator caused the greatest reduction of E. faecalis. Diode laser and Er:YAG laser activation were superior to ultrasonics in dentinal tubule disinfection. |
Persistent Identifier | http://hdl.handle.net/10722/236059 |
ISSN | 2023 Impact Factor: 5.4 2023 SCImago Journal Rankings: 2.155 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Neelakantan, P. | - |
dc.contributor.author | Cheng, C. Q. | - |
dc.contributor.author | Mohanraj, R. | - |
dc.contributor.author | Sriraman, P. | - |
dc.contributor.author | Subbarao, C. | - |
dc.contributor.author | Sharma, S. | - |
dc.date.accessioned | 2016-11-10T07:12:05Z | - |
dc.date.available | 2016-11-10T07:12:05Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | International Endodontic Journal, 2015, v. 48, n. 6, p. 602-610 | - |
dc.identifier.issn | 0143-2885 | - |
dc.identifier.uri | http://hdl.handle.net/10722/236059 | - |
dc.description.abstract | © 2014 International Endodontic Journal.Aim: To investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of Enterococcus faecalis in vitro. Methodology: Root canals in 280 single-rooted teeth were instrumented using a rotary Ni-Ti system. Biofilms of E. faecalis were generated based on a previously established protocol. Samples were randomly divided into three experimental (n = 80) and one control (n = 40) group based on the irrigation protocol employed: group 1 (NaOCl + Etidronic acid), 1 : 1 mixture of 6% NaOCl and 18% etidronic acid; group 2 (NaOCl-EDTA), 3% NaOCl followed by 17% EDTA; group 3 (NaOCl-EDTA-NaOCl), 3% NaOCl followed by 17% EDTA and a final flush of 3% NaOCl. Saline served as the control. Samples were further divided into four subgroups (n = 20) based on the activation method: subgroup A, no activation; subgroup B, ultrasonic activation; group C, diode laser; group D, Er:YAG laser. Confocal laser scanning microscopy was used to assess bacterial viability in situ. Root dentine powder was obtained for determining the colony-forming units (CFU mL<sup>-1</sup>). Data were analysed by appropriate statistical analyses with P = 0.05. Results: All experimental irrigation protocols caused complete destruction of the biofilm in the root canal lumen. Within the dentinal tubules, all groups had a significantly higher percentage of dead bacteria than the saline control (P < 0.05). There was no significant difference between NaOCl + etidronic acid and NaOCl-EDTA-NaOCl (P > 0.05), whereas both groups brought about more bacterial reduction than NaOCl-EDTA (P < 0.05). There was no significant difference between diode laser and Er:YAG laser in any of the groups (P > 0.05). Both diode and Er:YAG laser were more effective than ultrasonic activation and conventional syringe irrigation in reducing E. fecalis biofilms (P < 0.05). Conclusions: The use of NaOCl after or in combination with a chelator caused the greatest reduction of E. faecalis. Diode laser and Er:YAG laser activation were superior to ultrasonics in dentinal tubule disinfection. | - |
dc.language | eng | - |
dc.relation.ispartof | International Endodontic Journal | - |
dc.subject | Continuous chelation | - |
dc.subject | Confocal microscopy | - |
dc.subject | Biofilm | - |
dc.subject | Enterococcus faecalis | - |
dc.subject | Soft chelation | - |
dc.subject | Sodium hypochlorite | - |
dc.title | Antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or Er: YAG laser in vitro | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1111/iej.12354 | - |
dc.identifier.scopus | eid_2-s2.0-84929131880 | - |
dc.identifier.volume | 48 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 602 | - |
dc.identifier.epage | 610 | - |
dc.identifier.eissn | 1365-2591 | - |
dc.identifier.isi | WOS:000354294300013 | - |
dc.identifier.issnl | 0143-2885 | - |