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Conference Paper: Determining the mechanism of CSR-1 RNAi pathway to organize holocentromere in Caenorhabditis elegans
Title | Determining the mechanism of CSR-1 RNAi pathway to organize holocentromere in Caenorhabditis elegans |
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Authors | |
Issue Date | 2016 |
Citation | The 7th Asia-Pacific C. elegans Meeting, Beijing, China, 25-29 June 2016. How to Cite? |
Abstract | CSR-1 RNAi pathway is required for the holocentromere organization and proper chromosome segregation in Caenorhabditis elegans. It remains obscure how this pathway functionally links to these two events. A previous study showed that germline genes, which are targets of CSR-1 RNAi pathway, are inversely correlated to the centromeric histone H3 protein variant, HCP-3, occupancy on chromatin. We propose that CSR-1 RNAi pathway may restrict HCP-3 localization on the centromere. To study how the CSR-1 RNAi pathway affects the centromere function, we have closely examined the centromeric protein localization following RNAi knockdown of the Argonaute csr-1. Our live imaging data shows that csr-1 knockdown does not affect the bipolar arrangement of HCP-3 on centromere but elevates the centromeric HCP-3 level. We propose that the increase in HCP-3 chromatin localization results in merotelic kinetochore attachment and thus leads to chromosome missegregation. We will determine if tethering CSR-1 onto an HCP-3-enriched region will reduce HCP-3 occupancy by ChIP-qPCR. This study will provide insights on how RNAi and non-coding RNA transcripts function in centromere organization and implicate how centromeres are defined. |
Persistent Identifier | http://hdl.handle.net/10722/235486 |
DC Field | Value | Language |
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dc.contributor.author | Wong, YYC | - |
dc.contributor.author | Yuen, KWY | - |
dc.date.accessioned | 2016-10-14T13:53:34Z | - |
dc.date.available | 2016-10-14T13:53:34Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The 7th Asia-Pacific C. elegans Meeting, Beijing, China, 25-29 June 2016. | - |
dc.identifier.uri | http://hdl.handle.net/10722/235486 | - |
dc.description.abstract | CSR-1 RNAi pathway is required for the holocentromere organization and proper chromosome segregation in Caenorhabditis elegans. It remains obscure how this pathway functionally links to these two events. A previous study showed that germline genes, which are targets of CSR-1 RNAi pathway, are inversely correlated to the centromeric histone H3 protein variant, HCP-3, occupancy on chromatin. We propose that CSR-1 RNAi pathway may restrict HCP-3 localization on the centromere. To study how the CSR-1 RNAi pathway affects the centromere function, we have closely examined the centromeric protein localization following RNAi knockdown of the Argonaute csr-1. Our live imaging data shows that csr-1 knockdown does not affect the bipolar arrangement of HCP-3 on centromere but elevates the centromeric HCP-3 level. We propose that the increase in HCP-3 chromatin localization results in merotelic kinetochore attachment and thus leads to chromosome missegregation. We will determine if tethering CSR-1 onto an HCP-3-enriched region will reduce HCP-3 occupancy by ChIP-qPCR. This study will provide insights on how RNAi and non-coding RNA transcripts function in centromere organization and implicate how centromeres are defined. | - |
dc.language | eng | - |
dc.relation.ispartof | 7th Asia Pacific C. elegans Meeting | - |
dc.title | Determining the mechanism of CSR-1 RNAi pathway to organize holocentromere in Caenorhabditis elegans | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yuen, KWY: kwyyuen@hku.hk | - |
dc.identifier.authority | Yuen, KWY=rp01512 | - |
dc.identifier.hkuros | 268846 | - |