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Conference Paper: Annexin II-binding immunoglobulin level correlates with renal histological features in lupus nephritis
Title | Annexin II-binding immunoglobulin level correlates with renal histological features in lupus nephritis |
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Authors | |
Issue Date | 2016 |
Publisher | American Society of Nephrology. The Journal's web site is located at http://www.jasn.org |
Citation | The 2016 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2016), Chicago, IL., 15-20 November 2016. In Journal of the American Society of Nephrology, 2016, v. 27 abstract suppl., p. 392A, abstract FR-PO119 How to Cite? |
Abstract | BACKGROUND: Annexin II on the surface of mesangial cells mediates anti-dsDNA antibody binding and downstream inflammatory and fibrotic processes in lupus nephritis. We investigated the relationship between annexin II-binding immunoglobulins and renal histology in lupus nephritis. METHODS: Archived serial serum samples from 28 patients with Class III/IV lupus nephritis were retrieved, and annexin II-binding immunoglobulin level was measured with an in-house ELISA. Glomeruli were isolated from NZBWF1 mice, and annexin II gene and protein expression was investigated by real-time PCR and cytochemical staining respectively. Ultrastructural localization of annexin II was determined by immunogold staining and electron microscopy. RESULTS: Associations between serum annexin II-binding IgG level, anti-dsDNA level, and disease activity were observed in 42% of lupus nephritis patients. Annexin II-binding IgG level correlated with activity index (r=0.44, p=0.04), leukocyte infiltration score (r=0.52, p=0.01), and karyorrhexis/fibrinoid necrosis score (r=0.66, p=0.002) as stated in the patients’ renal biopsy reports, and also the semi-quantitative mesangial electron-dense deposit score (r=0.63, p=0.009). Glomerular annexin II expression increased with active nephritis and decreased with glomerulosclerosis in NZBWF1 mice. Annexin II expression co-localized with electron-dense deposits in the mesangium, along the glomerular basement membrane and around podocytes. CONCLUSIONS: The association between annexin II-binding IgG level and histological features of severe nephritis and the co-localization of annexin II with electron-dense deposits both implicate annexin II in the pathogenesis of lupus nephritis. Funding: Government Support ‑ Non-U.S. |
Description | Poster/Friday: Basic/Experimental Immunology - 1: no. FR-PO119 |
Persistent Identifier | http://hdl.handle.net/10722/235033 |
ISSN | 2023 Impact Factor: 10.3 2023 SCImago Journal Rankings: 3.409 |
DC Field | Value | Language |
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dc.contributor.author | Cheung, KF | - |
dc.contributor.author | Yung, SSY | - |
dc.contributor.author | Chau, KM | - |
dc.contributor.author | Yap, YHD | - |
dc.contributor.author | Chan, DTM | - |
dc.date.accessioned | 2016-10-14T13:50:50Z | - |
dc.date.available | 2016-10-14T13:50:50Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The 2016 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2016), Chicago, IL., 15-20 November 2016. In Journal of the American Society of Nephrology, 2016, v. 27 abstract suppl., p. 392A, abstract FR-PO119 | - |
dc.identifier.issn | 1046-6673 | - |
dc.identifier.uri | http://hdl.handle.net/10722/235033 | - |
dc.description | Poster/Friday: Basic/Experimental Immunology - 1: no. FR-PO119 | - |
dc.description.abstract | BACKGROUND: Annexin II on the surface of mesangial cells mediates anti-dsDNA antibody binding and downstream inflammatory and fibrotic processes in lupus nephritis. We investigated the relationship between annexin II-binding immunoglobulins and renal histology in lupus nephritis. METHODS: Archived serial serum samples from 28 patients with Class III/IV lupus nephritis were retrieved, and annexin II-binding immunoglobulin level was measured with an in-house ELISA. Glomeruli were isolated from NZBWF1 mice, and annexin II gene and protein expression was investigated by real-time PCR and cytochemical staining respectively. Ultrastructural localization of annexin II was determined by immunogold staining and electron microscopy. RESULTS: Associations between serum annexin II-binding IgG level, anti-dsDNA level, and disease activity were observed in 42% of lupus nephritis patients. Annexin II-binding IgG level correlated with activity index (r=0.44, p=0.04), leukocyte infiltration score (r=0.52, p=0.01), and karyorrhexis/fibrinoid necrosis score (r=0.66, p=0.002) as stated in the patients’ renal biopsy reports, and also the semi-quantitative mesangial electron-dense deposit score (r=0.63, p=0.009). Glomerular annexin II expression increased with active nephritis and decreased with glomerulosclerosis in NZBWF1 mice. Annexin II expression co-localized with electron-dense deposits in the mesangium, along the glomerular basement membrane and around podocytes. CONCLUSIONS: The association between annexin II-binding IgG level and histological features of severe nephritis and the co-localization of annexin II with electron-dense deposits both implicate annexin II in the pathogenesis of lupus nephritis. Funding: Government Support ‑ Non-U.S. | - |
dc.language | eng | - |
dc.publisher | American Society of Nephrology. The Journal's web site is located at http://www.jasn.org | - |
dc.relation.ispartof | Journal of the American Society of Nephrology | - |
dc.title | Annexin II-binding immunoglobulin level correlates with renal histological features in lupus nephritis | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Cheung, KF: skfc819@hku.hk | - |
dc.identifier.email | Yung, SSY: ssyyung@hku.hk | - |
dc.identifier.email | Chau, KM: melchau@hkucc.hku.hk | - |
dc.identifier.email | Yap, YHD: desmondy@hku.hk | - |
dc.identifier.email | Chan, DTM: dtmchan@hkucc.hku.hk | - |
dc.identifier.authority | Yung, SSY=rp00455 | - |
dc.identifier.authority | Yap, YHD=rp01607 | - |
dc.identifier.authority | Chan, DTM=rp00394 | - |
dc.identifier.hkuros | 269038 | - |
dc.identifier.volume | 27 | - |
dc.identifier.issue | abstract suppl. | - |
dc.identifier.spage | 392A, abstract FR-PO119 | - |
dc.identifier.epage | 392A, abstract FR-PO119 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1046-6673 | - |