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- Publisher Website: 10.1039/c5an01432h
- Scopus: eid_2-s2.0-84947093973
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Article: Facile synthesis of novel magnetic silica nanoparticles functionalized with layer-by-layer detonation nanodiamonds for secretome study.
Title | Facile synthesis of novel magnetic silica nanoparticles functionalized with layer-by-layer detonation nanodiamonds for secretome study. |
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Authors | |
Issue Date | 2015 |
Publisher | Royal Society of Chemistry. |
Citation | Analyst, 2015, v. 140 n. 23, p. 7886-7895 How to Cite? |
Abstract | Novel magnetic silica nanoparticles functionalized with layer-by-layer detonation nanodiamonds (dNDs) were prepared by coating single submicron-size magnetite particles with silica and subsequently modified with dNDs. The resulting layer-by-layer dND functionalized magnetic silica microspheres (Fe3O4@SiO2@[dND]n) exhibit a well-defined magnetite–core–silica–shell structure and possess a high content of magnetite, which endow them with high dispersibility and excellent magnetic responsibility. Meanwhile, dNDs are known for their high affinity and biocompatibility towards peptides or proteins. Thus, a novel convenient, fast and efficient pretreatment approach of low-abundance peptides or proteins was successfully established with Fe3O4@SiO2@[dND]n microspheres. The signal intensity of low-abundance peptides was improved by at least two to three orders of magnitude in mass spectrometry analysis. The novel microsphere also showed good tolerance to salt. Even with a high concentration of salt, peptides or proteins could be isolated effectively from samples. Therefore, the convenient and efficient enrichment process of this novel layer-by-layer dND-functionalized microsphere makes it a promising candidate for isolation of protein in a large volume of culture supernatant for secretome analysis. In the application of Fe3O4@SiO2@[dND]n in the secretome of hepatoma cells, 1473 proteins were identified and covered a broad range of pI and molecular weight, including 377 low molecular weight proteins. |
Persistent Identifier | http://hdl.handle.net/10722/234392 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 0.693 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, H | - |
dc.contributor.author | Wang, Y | - |
dc.contributor.author | Zhang, L | - |
dc.contributor.author | Lu, H | - |
dc.contributor.author | Zhou, Z | - |
dc.contributor.author | Wei, L | - |
dc.contributor.author | Yang, P | - |
dc.date.accessioned | 2016-10-14T13:46:33Z | - |
dc.date.available | 2016-10-14T13:46:33Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Analyst, 2015, v. 140 n. 23, p. 7886-7895 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | http://hdl.handle.net/10722/234392 | - |
dc.description.abstract | Novel magnetic silica nanoparticles functionalized with layer-by-layer detonation nanodiamonds (dNDs) were prepared by coating single submicron-size magnetite particles with silica and subsequently modified with dNDs. The resulting layer-by-layer dND functionalized magnetic silica microspheres (Fe3O4@SiO2@[dND]n) exhibit a well-defined magnetite–core–silica–shell structure and possess a high content of magnetite, which endow them with high dispersibility and excellent magnetic responsibility. Meanwhile, dNDs are known for their high affinity and biocompatibility towards peptides or proteins. Thus, a novel convenient, fast and efficient pretreatment approach of low-abundance peptides or proteins was successfully established with Fe3O4@SiO2@[dND]n microspheres. The signal intensity of low-abundance peptides was improved by at least two to three orders of magnitude in mass spectrometry analysis. The novel microsphere also showed good tolerance to salt. Even with a high concentration of salt, peptides or proteins could be isolated effectively from samples. Therefore, the convenient and efficient enrichment process of this novel layer-by-layer dND-functionalized microsphere makes it a promising candidate for isolation of protein in a large volume of culture supernatant for secretome analysis. In the application of Fe3O4@SiO2@[dND]n in the secretome of hepatoma cells, 1473 proteins were identified and covered a broad range of pI and molecular weight, including 377 low molecular weight proteins. | - |
dc.language | eng | - |
dc.publisher | Royal Society of Chemistry. | - |
dc.relation.ispartof | Analyst | - |
dc.title | Facile synthesis of novel magnetic silica nanoparticles functionalized with layer-by-layer detonation nanodiamonds for secretome study. | - |
dc.type | Article | - |
dc.identifier.email | Zhou, Z: zhongjun@hku.hk | - |
dc.identifier.authority | Zhou, Z=rp00503 | - |
dc.identifier.doi | 10.1039/c5an01432h | - |
dc.identifier.scopus | eid_2-s2.0-84947093973 | - |
dc.identifier.hkuros | 268134 | - |
dc.identifier.volume | 140 | - |
dc.identifier.issue | 23 | - |
dc.identifier.spage | 7886 | - |
dc.identifier.epage | 7895 | - |
dc.identifier.eissn | 1364-5528 | - |
dc.identifier.isi | WOS:000365189900007 | - |
dc.identifier.issnl | 0003-2654 | - |