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Conference Paper: Orexin modulates inhibitory synaptic transmission of vestibular nuclear neurons in rats
Title | Orexin modulates inhibitory synaptic transmission of vestibular nuclear neurons in rats |
---|---|
Authors | |
Issue Date | 2016 |
Publisher | The University of Hong Kong. |
Citation | The 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 37, abstract no. P16 How to Cite? |
Abstract | Orexin is a neuromodulator known to be produced by lateral hypothalamic neurons, which send projections to the
hippocampus, brainstem and cerebellum. In the hippocampus, orexin is known to modulate long-term synaptic plasticity,
thereby contributing to social memory. We hypothesized that orexin modulates synaptic transmission in the vestibular
nucleus (VN), thus regulating behavioral expression. To understand the impact of orexin on synaptic transmission within
the VN, we employed an established in vitro electrophysiological technique to study the action of orexin on the excitability
of neurons in the medial vestibular nucleus (MVN) of rats at postnatal day 14. Whole-cell patch-clamp results indicated
that treatment with orexin led to reductions in amplitude and frequency of miniature inhibitory postsynaptic current
(mIPSC). This suggests that orexin decreases both the presynaptic release of inhibitory transmitters and postsynaptic
depolarization in MVN neurons. We thus demonstrated that orexin attenuates synaptic inhibition on MVN neurons. We
further tested whether orexin-modulated mIPSC is mediated by GABAA receptors or glycine receptors. With the use of
bicuculline and strychnine, we observed that orexin decreased mIPSC mediated by GABAA receptors, but not glycine
receptors. Taken together, our findings provided us with fundamental knowledge about the modulatory role of orexinergic
transmission in the VN. This forms the basis for further investigation on the involvement of orexin in long-term synaptic
plasticity of the central vestibular system. [Supported by N_HKU735/14] |
Description | Conference Theme: Nature and Nurture in Brain Functions |
Persistent Identifier | http://hdl.handle.net/10722/231498 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiang, Y | - |
dc.contributor.author | Ma, CW | - |
dc.contributor.author | Wang, JJ | - |
dc.contributor.author | Chan, YS | - |
dc.date.accessioned | 2016-09-20T05:23:33Z | - |
dc.date.available | 2016-09-20T05:23:33Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 37, abstract no. P16 | - |
dc.identifier.uri | http://hdl.handle.net/10722/231498 | - |
dc.description | Conference Theme: Nature and Nurture in Brain Functions | - |
dc.description.abstract | Orexin is a neuromodulator known to be produced by lateral hypothalamic neurons, which send projections to the hippocampus, brainstem and cerebellum. In the hippocampus, orexin is known to modulate long-term synaptic plasticity, thereby contributing to social memory. We hypothesized that orexin modulates synaptic transmission in the vestibular nucleus (VN), thus regulating behavioral expression. To understand the impact of orexin on synaptic transmission within the VN, we employed an established in vitro electrophysiological technique to study the action of orexin on the excitability of neurons in the medial vestibular nucleus (MVN) of rats at postnatal day 14. Whole-cell patch-clamp results indicated that treatment with orexin led to reductions in amplitude and frequency of miniature inhibitory postsynaptic current (mIPSC). This suggests that orexin decreases both the presynaptic release of inhibitory transmitters and postsynaptic depolarization in MVN neurons. We thus demonstrated that orexin attenuates synaptic inhibition on MVN neurons. We further tested whether orexin-modulated mIPSC is mediated by GABAA receptors or glycine receptors. With the use of bicuculline and strychnine, we observed that orexin decreased mIPSC mediated by GABAA receptors, but not glycine receptors. Taken together, our findings provided us with fundamental knowledge about the modulatory role of orexinergic transmission in the VN. This forms the basis for further investigation on the involvement of orexin in long-term synaptic plasticity of the central vestibular system. [Supported by N_HKU735/14] | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong. | - |
dc.relation.ispartof | Neuroscience Symposium & HKSN 2016 Annual Scientific Conference | - |
dc.title | Orexin modulates inhibitory synaptic transmission of vestibular nuclear neurons in rats | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Ma, CW: cwma2010@hku.hk | - |
dc.identifier.email | Wang, JJ: junwen@hku.hk | - |
dc.identifier.email | Chan, YS: yschan@hku.hk | - |
dc.identifier.authority | Wang, JJ=rp00280 | - |
dc.identifier.authority | Chan, YS=rp00318 | - |
dc.identifier.hkuros | 266238 | - |
dc.identifier.hkuros | 267893 | - |
dc.identifier.spage | 37, abstract no. P16 | - |
dc.identifier.epage | 37, abstract no. P16 | - |
dc.publisher.place | Hong Kong | - |