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Conference Paper: Schwann cells demonstrated lineage plasticity in culture to acquire oligodendrocyte phenotypes
Title | Schwann cells demonstrated lineage plasticity in culture to acquire oligodendrocyte phenotypes |
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Authors | |
Issue Date | 2016 |
Publisher | The University of Hong Kong. |
Citation | The 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences (HKSN), The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 23, abstract no. OP5 How to Cite? |
Abstract | Schwann cells and oligodendrocytes are myelin-forming glial cells found in the peripheral and central nervous systems,
respectively. Despite being similar in function, the two cell types have distinct developmental origins. In cultured Schwann
cells, however, we serendipitously observed expression of the oligodendrocyte lineage fate determining factor
Olig2. Purified Schwann cell cultures were prepared from neonatal rat sciatic nerves. Olig2-positive Schwann cells (OL2-
SCs) were detected at 25-30 DIV. By 50-60 DIV, OL2-SCs acquired polydendritic morphology typical of oligodendrocyte
precursors. This was accompanied by a decline in Schwann cell marker expression. The OP-like cells were termed
Schwann cell-derived oligodendrocyte precursors (SC-OP). We therefore hypothesized that the peripheral nervous system
environment is essential for the maintenance of Schwann cell identity. Co-culture of OL2-SCs with dorsal root ganglia
neurons prevented conversion in SC-OPs. In contrast, SC-OPs co-cultured with dorsal root ganglia neurons continued
differentiation into mature oligodendrocyte-like cells with myelin basic protein-positive segments along multiple
axons. Our results revealed 'lineage switching' capability of Schwann cells isolated from the peripheral nervous
system. Preservation of Schwann cell identity in vitro requires signalling cues derived from peripheral neurons. |
Description | Conference Theme: Nature and Nurture in Brain Functions |
Persistent Identifier | http://hdl.handle.net/10722/231466 |
DC Field | Value | Language |
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dc.contributor.author | Tsui, YP | - |
dc.contributor.author | Tam, KW | - |
dc.contributor.author | Shum, DKY | - |
dc.contributor.author | Chan, YS | - |
dc.date.accessioned | 2016-09-20T05:23:19Z | - |
dc.date.available | 2016-09-20T05:23:19Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences (HKSN), The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 23, abstract no. OP5 | - |
dc.identifier.uri | http://hdl.handle.net/10722/231466 | - |
dc.description | Conference Theme: Nature and Nurture in Brain Functions | - |
dc.description.abstract | Schwann cells and oligodendrocytes are myelin-forming glial cells found in the peripheral and central nervous systems, respectively. Despite being similar in function, the two cell types have distinct developmental origins. In cultured Schwann cells, however, we serendipitously observed expression of the oligodendrocyte lineage fate determining factor Olig2. Purified Schwann cell cultures were prepared from neonatal rat sciatic nerves. Olig2-positive Schwann cells (OL2- SCs) were detected at 25-30 DIV. By 50-60 DIV, OL2-SCs acquired polydendritic morphology typical of oligodendrocyte precursors. This was accompanied by a decline in Schwann cell marker expression. The OP-like cells were termed Schwann cell-derived oligodendrocyte precursors (SC-OP). We therefore hypothesized that the peripheral nervous system environment is essential for the maintenance of Schwann cell identity. Co-culture of OL2-SCs with dorsal root ganglia neurons prevented conversion in SC-OPs. In contrast, SC-OPs co-cultured with dorsal root ganglia neurons continued differentiation into mature oligodendrocyte-like cells with myelin basic protein-positive segments along multiple axons. Our results revealed 'lineage switching' capability of Schwann cells isolated from the peripheral nervous system. Preservation of Schwann cell identity in vitro requires signalling cues derived from peripheral neurons. | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong. | - |
dc.relation.ispartof | Neuroscience Symposium & HKSN 2016 Annual Scientific Conference | - |
dc.title | Schwann cells demonstrated lineage plasticity in culture to acquire oligodendrocyte phenotypes | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Tsui, YP: alex2013@hku.hk | - |
dc.identifier.email | Tam, KW: tamkw@hku.hk | - |
dc.identifier.email | Shum, DKY: shumdkhk@hkucc.hku.hk | - |
dc.identifier.email | Chan, YS: yschan@hku.hk | - |
dc.identifier.authority | Shum, DKY=rp00321 | - |
dc.identifier.authority | Chan, YS=rp00318 | - |
dc.identifier.hkuros | 263578 | - |
dc.identifier.spage | 23, abstract no. OP5 | - |
dc.identifier.epage | 23, abstract no. OP5 | - |
dc.publisher.place | Hong Kong | - |