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Article: Middle East Respiratory Syndrome Coronavirus Efficiently Infects Human Primary T Lymphocytes and Activates the Extrinsic and Intrinsic Apoptosis Pathways

TitleMiddle East Respiratory Syndrome Coronavirus Efficiently Infects Human Primary T Lymphocytes and Activates the Extrinsic and Intrinsic Apoptosis Pathways
Authors
KeywordsMERS-CoV
T lymphocytes
Apoptosis
Caspase
Tonsil
Spleen
Marmosets
Issue Date2016
PublisherOxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/jid/
Citation
Journal of Infectious Diseases, 2016, v. 213 n. 6, p. 904-914 How to Cite?
AbstractMiddle East respiratory syndrome (MERS) is associated with a mortality rate of >35%. We previously showed that MERS coronavirus (MERS-CoV) could infect human macrophages and dendritic cells and induce cytokine dysregulation. Here, we further investigated the interplay between human primary T cells and MERS-CoV in disease pathogenesis. Importantly, our results suggested that MERS-CoV efficiently infected T cells from the peripheral blood and from human lymphoid organs, including the spleen and the tonsil. We further demonstrated that MERS-CoV infection induced apoptosis in T cells, which involved the activation of both the extrinsic and intrinsic apoptosis pathways. Remarkably, immunostaining of spleen sections from MERS-CoV–infected common marmosets demonstrated the presence of viral nucleoprotein in their CD3+ T cells. Overall, our results suggested that the unusual capacity of MERS-CoV to infect T cells and induce apoptosis might partly contribute to the high pathogenicity of the virus.
Persistent Identifierhttp://hdl.handle.net/10722/231185
ISSN
2020 Impact Factor: 5.226
2015 SCImago Journal Rankings: 4.000
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChu, H-
dc.contributor.authorZhou, J-
dc.contributor.authorWong, HYB-
dc.contributor.authorLi, C-
dc.contributor.authorChan, JFW-
dc.contributor.authorCheng, Z-
dc.contributor.authorYang, D-
dc.contributor.authorWang, D-
dc.contributor.authorLee, CY-
dc.contributor.authorLi, C-
dc.contributor.authorYeung, ML-
dc.contributor.authorCai, J-
dc.contributor.authorChan, HYI-
dc.contributor.authorHo, WK-
dc.contributor.authorTo, KKW-
dc.contributor.authorZheng, B-
dc.contributor.authorYao, Y-
dc.contributor.authorQin, C-
dc.contributor.authorYuen, KY-
dc.date.accessioned2016-09-20T05:21:16Z-
dc.date.available2016-09-20T05:21:16Z-
dc.date.issued2016-
dc.identifier.citationJournal of Infectious Diseases, 2016, v. 213 n. 6, p. 904-914-
dc.identifier.issn0022-1899-
dc.identifier.urihttp://hdl.handle.net/10722/231185-
dc.description.abstractMiddle East respiratory syndrome (MERS) is associated with a mortality rate of >35%. We previously showed that MERS coronavirus (MERS-CoV) could infect human macrophages and dendritic cells and induce cytokine dysregulation. Here, we further investigated the interplay between human primary T cells and MERS-CoV in disease pathogenesis. Importantly, our results suggested that MERS-CoV efficiently infected T cells from the peripheral blood and from human lymphoid organs, including the spleen and the tonsil. We further demonstrated that MERS-CoV infection induced apoptosis in T cells, which involved the activation of both the extrinsic and intrinsic apoptosis pathways. Remarkably, immunostaining of spleen sections from MERS-CoV–infected common marmosets demonstrated the presence of viral nucleoprotein in their CD3+ T cells. Overall, our results suggested that the unusual capacity of MERS-CoV to infect T cells and induce apoptosis might partly contribute to the high pathogenicity of the virus.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/jid/-
dc.relation.ispartofJournal of Infectious Diseases-
dc.subjectMERS-CoV-
dc.subjectT lymphocytes-
dc.subjectApoptosis-
dc.subjectCaspase-
dc.subjectTonsil-
dc.subjectSpleen-
dc.subjectMarmosets-
dc.titleMiddle East Respiratory Syndrome Coronavirus Efficiently Infects Human Primary T Lymphocytes and Activates the Extrinsic and Intrinsic Apoptosis Pathways-
dc.typeArticle-
dc.identifier.emailChu, H: hinchu@hku.hk-
dc.identifier.emailZhou, J: jiezhou@hku.hk-
dc.identifier.emailLi, C: licun@HKUCC-COM.hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailYang, D: dongfang@hku.hk-
dc.identifier.emailWang, D: wdfly@HKUCC-COM.hku.hk-
dc.identifier.emailYeung, ML: pmlyeung@hku.hk-
dc.identifier.emailCai, J: caijuice@hku.hk-
dc.identifier.emailChan, HYI: ivyhchan@hku.hk-
dc.identifier.emailHo, WK: wkho@hkucc.hku.hk-
dc.identifier.emailTo, KKW: kelvinto@hkucc.hku.hk-
dc.identifier.emailZheng, B: bzheng@hkucc.hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityChu, H=rp02125-
dc.identifier.authorityZhou, J=rp01412-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authorityYeung, ML=rp01402-
dc.identifier.authorityTo, KKW=rp01384-
dc.identifier.authorityZheng, B=rp00353-
dc.identifier.authorityYuen, KY=rp00366-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/infdis/jiv380-
dc.identifier.pmid26203058-
dc.identifier.pmcidPMC7107330-
dc.identifier.scopuseid_2-s2.0-84964848410-
dc.identifier.hkuros266249-
dc.identifier.volume213-
dc.identifier.issue6-
dc.identifier.spage904-
dc.identifier.epage914-
dc.identifier.eissn1537-6613-
dc.identifier.isiWOS:000374186000006-
dc.publisher.placeUnited States-
dc.identifier.issnl0022-1899-

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