File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Gene expression profiling of bone cells on smooth and rough titanium surfaces.

TitleGene expression profiling of bone cells on smooth and rough titanium surfaces.
Authors
Issue Date2004
Citation
Journal of materials science. Materials in medicine, 2004, v. 15, n. 11, p. 1255-1258 How to Cite?
AbstractTitanium (Ti) and Ti alloys are widely used as dental and orthopaedic implants, but the effects of the surface characteristics of these materials on the response of cells and target tissues is not well understood. The present study has therefore examined the effects of a rough Ti (RT) and a smooth Ti (ST) surface on human bone cells in vitro. Scanning electron microscopy showed attachment and spreading of cells on both surfaces. Expression profiling using ATLAS gene arrays showed marked differences in gene responses after 3 h of culture. A number of osteoblast genes were identified as "roughness response" genes on the basis of changes in expression on the RT compared with the ST surfaces. The surface roughness of Ti was thus found to have a profound effect on the profile of genes expressed by the bone cells, and suggests that improvements in the biological activity and possibly the clinical efficacy of these materials could be achieved by selective regulation of gene expression mediated by controlled modification of Ti surface.
Persistent Identifierhttp://hdl.handle.net/10722/230742
ISSN
2023 Impact Factor: 4.2
2023 SCImago Journal Rankings: 0.651
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHarle, J.-
dc.contributor.authorSalih, V.-
dc.contributor.authorOlsen, I.-
dc.contributor.authorBrett, P.-
dc.contributor.authorJones, F.-
dc.contributor.authorTonetti, M.-
dc.date.accessioned2016-09-01T06:06:41Z-
dc.date.available2016-09-01T06:06:41Z-
dc.date.issued2004-
dc.identifier.citationJournal of materials science. Materials in medicine, 2004, v. 15, n. 11, p. 1255-1258-
dc.identifier.issn0957-4530-
dc.identifier.urihttp://hdl.handle.net/10722/230742-
dc.description.abstractTitanium (Ti) and Ti alloys are widely used as dental and orthopaedic implants, but the effects of the surface characteristics of these materials on the response of cells and target tissues is not well understood. The present study has therefore examined the effects of a rough Ti (RT) and a smooth Ti (ST) surface on human bone cells in vitro. Scanning electron microscopy showed attachment and spreading of cells on both surfaces. Expression profiling using ATLAS gene arrays showed marked differences in gene responses after 3 h of culture. A number of osteoblast genes were identified as "roughness response" genes on the basis of changes in expression on the RT compared with the ST surfaces. The surface roughness of Ti was thus found to have a profound effect on the profile of genes expressed by the bone cells, and suggests that improvements in the biological activity and possibly the clinical efficacy of these materials could be achieved by selective regulation of gene expression mediated by controlled modification of Ti surface.-
dc.languageeng-
dc.relation.ispartofJournal of materials science. Materials in medicine-
dc.titleGene expression profiling of bone cells on smooth and rough titanium surfaces.-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid15880936-
dc.identifier.scopuseid_2-s2.0-22444432898-
dc.identifier.volume15-
dc.identifier.issue11-
dc.identifier.spage1255-
dc.identifier.epage1258-
dc.identifier.isiWOS:000226811900013-
dc.identifier.issnl0957-4530-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats