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Conference Paper: Nuclear met-derived exosomes promote HCC metastasis and formation of lung premetastatic niche
Title | Nuclear met-derived exosomes promote HCC metastasis and formation of lung premetastatic niche |
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Authors | |
Issue Date | 2016 |
Publisher | Springer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0 |
Citation | The 25th Conference of the Asian Pacific Association for the Study of the Liver (APASL 2016), Tokyo Japan, 20-24 February 2016. In Hepatology International, 2016, v. 10 n. 1 suppl., p. S292, abstract no. P0559 How to Cite? |
Abstract | Met receptor tyrosine kinase triggers a wide range of normal physiological signaling cascades. However, aberration activation of the Met is commonly found in human cancers. Emerging evidence has shown that nuclear Met (nMet) is expressed in some cancerous tissues and cell lines, suggesting nMet could have unexplored functions in the nucleus. Our previous studies have provided the first evidence about the clinical relevance of nMet in human hepatocellular carcinoma (HCC). Functionally, nMet promoted HCC tumorigenesis and metastasis. In our continual study to elucidate the underlying mechanism of nMet in driving HCC metastasis, we explored the significance of exosomes in HCC. In our present study, exosomes were isolated from metastatic MHCC97L HCC cell in which nMet was overexpressed. Electron microscopy images of the exosomes isolated from the conditioned media of control MHCC97L/Vec and MHCC97L/nMet cells showed typical exosome structure with diameter of approximately 50–80 nm. Immunoblotting showed that the isolated exosomes were highly enriched in exosomal markers while depleted of the cytoskeletal protein beta-tubulin, cis-Golgi marker GM130 and nucleoporin p62. Functional assays showed that exosomes derived from MHCC97L/nMet cells significantly augmented both the migratory and invasive properties of normal liver and naı¨ve HCC cells. The uptake of PKH26-labeled nMet-exosomes by naı¨ve cells were investigated by fluorescence microscopy. In animal model, intravenous injection of nMet-exosomes enhanced the incidence of distant metastasis from HCC primary tumor to lungs. Our findings will provide an important paradigm about tumor-derived exosomes in driving metastasis and yield novel mechanistic insights into liver cancer metastasis. |
Description | Poster Presentation: P-0559 This journal suppl. entitled: Abstracts of the 25th Annual Conference of APASL, February 20–24, 2016, Tokyo, Japan |
Persistent Identifier | http://hdl.handle.net/10722/229935 |
ISSN | 2023 Impact Factor: 5.9 2023 SCImago Journal Rankings: 1.813 |
DC Field | Value | Language |
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dc.contributor.author | Yam, JWP | - |
dc.contributor.author | Tey, SK | - |
dc.contributor.author | Mao, X | - |
dc.date.accessioned | 2016-08-23T14:14:11Z | - |
dc.date.available | 2016-08-23T14:14:11Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The 25th Conference of the Asian Pacific Association for the Study of the Liver (APASL 2016), Tokyo Japan, 20-24 February 2016. In Hepatology International, 2016, v. 10 n. 1 suppl., p. S292, abstract no. P0559 | - |
dc.identifier.issn | 1936-0533 | - |
dc.identifier.uri | http://hdl.handle.net/10722/229935 | - |
dc.description | Poster Presentation: P-0559 | - |
dc.description | This journal suppl. entitled: Abstracts of the 25th Annual Conference of APASL, February 20–24, 2016, Tokyo, Japan | - |
dc.description.abstract | Met receptor tyrosine kinase triggers a wide range of normal physiological signaling cascades. However, aberration activation of the Met is commonly found in human cancers. Emerging evidence has shown that nuclear Met (nMet) is expressed in some cancerous tissues and cell lines, suggesting nMet could have unexplored functions in the nucleus. Our previous studies have provided the first evidence about the clinical relevance of nMet in human hepatocellular carcinoma (HCC). Functionally, nMet promoted HCC tumorigenesis and metastasis. In our continual study to elucidate the underlying mechanism of nMet in driving HCC metastasis, we explored the significance of exosomes in HCC. In our present study, exosomes were isolated from metastatic MHCC97L HCC cell in which nMet was overexpressed. Electron microscopy images of the exosomes isolated from the conditioned media of control MHCC97L/Vec and MHCC97L/nMet cells showed typical exosome structure with diameter of approximately 50–80 nm. Immunoblotting showed that the isolated exosomes were highly enriched in exosomal markers while depleted of the cytoskeletal protein beta-tubulin, cis-Golgi marker GM130 and nucleoporin p62. Functional assays showed that exosomes derived from MHCC97L/nMet cells significantly augmented both the migratory and invasive properties of normal liver and naı¨ve HCC cells. The uptake of PKH26-labeled nMet-exosomes by naı¨ve cells were investigated by fluorescence microscopy. In animal model, intravenous injection of nMet-exosomes enhanced the incidence of distant metastasis from HCC primary tumor to lungs. Our findings will provide an important paradigm about tumor-derived exosomes in driving metastasis and yield novel mechanistic insights into liver cancer metastasis. | - |
dc.language | eng | - |
dc.publisher | Springer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0 | - |
dc.relation.ispartof | Hepatology International | - |
dc.title | Nuclear met-derived exosomes promote HCC metastasis and formation of lung premetastatic niche | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yam, JWP: judyyam@pathology.hku.hk | - |
dc.identifier.email | Tey, SK: szekeong@hku.hk | - |
dc.identifier.email | Mao, X: susanmao@hku.hk | - |
dc.identifier.authority | Yam, JWP=rp00468 | - |
dc.description.nature | abstract | - |
dc.identifier.hkuros | 261344 | - |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 1 suppl. | - |
dc.identifier.spage | S292, abstract no. P0559 | - |
dc.identifier.epage | S292, abstract no. P0559 | - |
dc.publisher.place | United States | - |
dc.identifier.partofdoi | 10.1007/s12072-016-9707-8 | - |
dc.identifier.issnl | 1936-0533 | - |