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Conference Paper: IL-17 expression and its effect on osteoclastogenesis under mechanical loading
Title | IL-17 expression and its effect on osteoclastogenesis under mechanical loading |
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Authors | |
Issue Date | 2016 |
Publisher | International Association of Dental Research. The Abstracts' web site is located at https://iadr.abstractarchives.com/home |
Citation | The 94th General Session & Exhibition of the International Association for Dental Research (IADR), 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 1666 How to Cite? |
Abstract | OBJECTIVES: The molecular mechanisms of bone remodeling under mechanical loading is crucial for understanding alveolar bone metabolism. Interleukin (IL)-17 plays a key role in orchestrate osteoclast activation and differentiation. This study investigated the expression of IL-17 under mechanical force and its effect on osteoclastogenesis in osteocyte-like cells. METHODS: MLO-Y4 osteocytes were loaded with flow shear stress driven by Streamer® Shear Stress Device (4 dyn/cm2 and 16 dyn/cm2 respectively, 0.5 Hz, 2h). IL-17 at 0.1, 1 and 10ng/ml stimulated the osteocytes for 24h. The IL-17, IL-17 receptor (IL-17R), osteoprotegerin (OPG) and the receptor activator of NF-κB ligand (RANKL) mRNA expressions were analyzed by real-time polymerase chain reaction (qPCR). Co-culture of osteocytes and bone marrow-derived macrophage cells (BMMs) was undertaken to investigate the effect of IL-17 on osteoclastogenesis. Conditional medium (CM) from MLO-Y4 was collected 24h after force loading. Cells were co-cultured in normal medium or CM with or without IL-17 for 7 days. Tartrate-resistant acid phosphatase (TRAP) staining cells were quantified. RESULTS: The basal expression of IL-17 and IL-17R in MLO-Y4 osteocytes was up-regulated under shear stress loading. IL-17 significantly enhanced the mRNA expression of RANKL while decreased the ratio of OPG/RANKL. In co-culture of MLO-Y4 and BMMs, IL-17 significantly increased the formation of TRAP+ osteoclasts, while the CM from shear stress loaded MLO-Y4 inhibited the stimulatory effect of IL-17 on osteoclastogenesis. CONCLUSIONS: Mechanical loading enhances the expression of IL-17 and IL-17R in MLO-Y4 osteocytes. IL-17 promotes osteoclastogenisis, whereas such effect seems to be weakened under shear stress stimulation. Further study on the exact underlying mechanisms is warranted. |
Description | Poster Session - Biology of Tooth Movement: no. 1666 |
Persistent Identifier | http://hdl.handle.net/10722/227497 |
DC Field | Value | Language |
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dc.contributor.author | Yang, Y | - |
dc.contributor.author | Liao, C | - |
dc.contributor.author | Jin, L | - |
dc.contributor.author | Zhang, C | - |
dc.date.accessioned | 2016-07-18T09:11:04Z | - |
dc.date.available | 2016-07-18T09:11:04Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The 94th General Session & Exhibition of the International Association for Dental Research (IADR), 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 1666 | - |
dc.identifier.uri | http://hdl.handle.net/10722/227497 | - |
dc.description | Poster Session - Biology of Tooth Movement: no. 1666 | - |
dc.description.abstract | OBJECTIVES: The molecular mechanisms of bone remodeling under mechanical loading is crucial for understanding alveolar bone metabolism. Interleukin (IL)-17 plays a key role in orchestrate osteoclast activation and differentiation. This study investigated the expression of IL-17 under mechanical force and its effect on osteoclastogenesis in osteocyte-like cells. METHODS: MLO-Y4 osteocytes were loaded with flow shear stress driven by Streamer® Shear Stress Device (4 dyn/cm2 and 16 dyn/cm2 respectively, 0.5 Hz, 2h). IL-17 at 0.1, 1 and 10ng/ml stimulated the osteocytes for 24h. The IL-17, IL-17 receptor (IL-17R), osteoprotegerin (OPG) and the receptor activator of NF-κB ligand (RANKL) mRNA expressions were analyzed by real-time polymerase chain reaction (qPCR). Co-culture of osteocytes and bone marrow-derived macrophage cells (BMMs) was undertaken to investigate the effect of IL-17 on osteoclastogenesis. Conditional medium (CM) from MLO-Y4 was collected 24h after force loading. Cells were co-cultured in normal medium or CM with or without IL-17 for 7 days. Tartrate-resistant acid phosphatase (TRAP) staining cells were quantified. RESULTS: The basal expression of IL-17 and IL-17R in MLO-Y4 osteocytes was up-regulated under shear stress loading. IL-17 significantly enhanced the mRNA expression of RANKL while decreased the ratio of OPG/RANKL. In co-culture of MLO-Y4 and BMMs, IL-17 significantly increased the formation of TRAP+ osteoclasts, while the CM from shear stress loaded MLO-Y4 inhibited the stimulatory effect of IL-17 on osteoclastogenesis. CONCLUSIONS: Mechanical loading enhances the expression of IL-17 and IL-17R in MLO-Y4 osteocytes. IL-17 promotes osteoclastogenisis, whereas such effect seems to be weakened under shear stress stimulation. Further study on the exact underlying mechanisms is warranted. | - |
dc.language | eng | - |
dc.publisher | International Association of Dental Research. The Abstracts' web site is located at https://iadr.abstractarchives.com/home | - |
dc.relation.ispartof | Journal of Dental Research (Spec Issue) | - |
dc.relation.ispartof | IADR/APR General Session & Exhibition 2016 | - |
dc.title | IL-17 expression and its effect on osteoclastogenesis under mechanical loading | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yang, Y: yangyanq@hku.hk | - |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.authority | Yang, Y=rp00045 | - |
dc.identifier.authority | Jin, L=rp00028 | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.identifier.hkuros | 259740 | - |
dc.identifier.hkuros | 329506 | - |
dc.identifier.volume | 95 | - |
dc.identifier.issue | Spec. Iss. B | - |
dc.publisher.place | United States | - |