File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Isolation and characterization of dromedary camel coronavirus UAE-HKU23 from dromedaries of the Middle East: minimal serological cross-reactivity between MERS coronavirus and dromedary camel coronavirus UAE-HKU23

TitleIsolation and characterization of dromedary camel coronavirus UAE-HKU23 from dromedaries of the Middle East: minimal serological cross-reactivity between MERS coronavirus and dromedary camel coronavirus UAE-HKU23
Authors
KeywordsCharacterization
Coronavirus
Dromedary camel
Isolation
Issue Date2016
PublisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms
Citation
International Journal of Molecular Sciences, 2016, v. 17 n. 5, article no. 691 How to Cite?
AbstractRecently, we reported the discovery of a dromedary camel coronavirus UAE-HKU23 (DcCoV UAE-HKU23) from dromedaries in the Middle East. In this study, DcCoV UAE-HKU23 was successfully isolated in two of the 14 dromedary fecal samples using HRT-18G cells, with cytopathic effects observed five days after inoculation. Northern blot analysis revealed at least seven distinct RNA species, corresponding to predicted subgenomic mRNAs and confirming the core sequence of transcription regulatory sequence motifs as 5ʹ-UCUAAAC-3ʹ as we predicted previously. Antibodies against DcCoV UAE-HKU23 were detected in 58 (98.3%) and 59 (100%) of the 59 dromedary sera by immunofluorescence and neutralization antibody tests, respectively. There was significant correlation between the antibody titers determined by immunofluorescence and neutralization assays (Pearson coefficient = 0.525, p < 0.0001). Immunization of mice using recombinant N proteins of DcCoV UAE-HKU23 and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, and heat-inactivated DcCoV UAE-HKU23 showed minimal cross-antigenicity between DcCoV UAE-HKU23 and MERS-CoV by Western blot and neutralization antibody assays. Codon usage and genetic distance analysis of RdRp, S and N genes showed that the 14 strains of DcCoV UAE-HKU23 formed a distinct cluster, separated from those of other closely related members of Betacoronavirus 1, including alpaca CoV, confirming that DcCoV UAE-HKU23 is a novel member of Betacoronavirus 1.
Persistent Identifierhttp://hdl.handle.net/10722/225636
ISSN
2018 Impact Factor: 4.183
2015 SCImago Journal Rankings: 1.160
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCY-
dc.contributor.authorLau, SKP-
dc.contributor.authorFan, RYY-
dc.contributor.authorLau, CCY-
dc.contributor.authorWong, EYM-
dc.contributor.authorJoseph, S-
dc.contributor.authorTsang, AKL-
dc.contributor.authorWernery, R-
dc.contributor.authorYip, CCY-
dc.contributor.authorTsang, CC-
dc.contributor.authorWernery, U-
dc.contributor.authorYuen, KY-
dc.date.accessioned2016-05-20T08:09:38Z-
dc.date.available2016-05-20T08:09:38Z-
dc.date.issued2016-
dc.identifier.citationInternational Journal of Molecular Sciences, 2016, v. 17 n. 5, article no. 691-
dc.identifier.issn1422-0067-
dc.identifier.urihttp://hdl.handle.net/10722/225636-
dc.description.abstractRecently, we reported the discovery of a dromedary camel coronavirus UAE-HKU23 (DcCoV UAE-HKU23) from dromedaries in the Middle East. In this study, DcCoV UAE-HKU23 was successfully isolated in two of the 14 dromedary fecal samples using HRT-18G cells, with cytopathic effects observed five days after inoculation. Northern blot analysis revealed at least seven distinct RNA species, corresponding to predicted subgenomic mRNAs and confirming the core sequence of transcription regulatory sequence motifs as 5ʹ-UCUAAAC-3ʹ as we predicted previously. Antibodies against DcCoV UAE-HKU23 were detected in 58 (98.3%) and 59 (100%) of the 59 dromedary sera by immunofluorescence and neutralization antibody tests, respectively. There was significant correlation between the antibody titers determined by immunofluorescence and neutralization assays (Pearson coefficient = 0.525, p < 0.0001). Immunization of mice using recombinant N proteins of DcCoV UAE-HKU23 and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, and heat-inactivated DcCoV UAE-HKU23 showed minimal cross-antigenicity between DcCoV UAE-HKU23 and MERS-CoV by Western blot and neutralization antibody assays. Codon usage and genetic distance analysis of RdRp, S and N genes showed that the 14 strains of DcCoV UAE-HKU23 formed a distinct cluster, separated from those of other closely related members of Betacoronavirus 1, including alpaca CoV, confirming that DcCoV UAE-HKU23 is a novel member of Betacoronavirus 1.-
dc.languageeng-
dc.publisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms-
dc.relation.ispartofInternational Journal of Molecular Sciences-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCharacterization-
dc.subjectCoronavirus-
dc.subjectDromedary camel-
dc.subjectIsolation-
dc.titleIsolation and characterization of dromedary camel coronavirus UAE-HKU23 from dromedaries of the Middle East: minimal serological cross-reactivity between MERS coronavirus and dromedary camel coronavirus UAE-HKU23-
dc.typeArticle-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailFan, RYY: rfyy@hku.hk-
dc.identifier.emailLau, CCY: laucandy@hku.hk-
dc.identifier.emailWong, EYM: emilyhk@hku.hk-
dc.identifier.emailTsang, AKL: tsangkl@HKUCC-COM.hku.hk-
dc.identifier.emailYip, CCY: yipcyril@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityWoo, PCY=rp00430-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityYip, CCY=rp01721-
dc.identifier.authorityYuen, KY=rp00366-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/ijms17050691-
dc.identifier.pmid27164099-
dc.identifier.scopuseid_2-s2.0-84966318763-
dc.identifier.hkuros257972-
dc.identifier.volume17-
dc.identifier.issue5-
dc.identifier.spagearticle no. 691-
dc.identifier.epagearticle no. 691-
dc.identifier.isiWOS:000378791400085-
dc.publisher.placeSwitzerland-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats