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Article: Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms

TitleSynergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms
Authors
KeywordsAntimicrobials
Chlorhexidine
Nanotechnology
Oral biofilms
Scutellaria baicalensis
Issue Date2016
PublisherMDPIAG. The Journal's web site is located at http://www.mdpi.com/journal/nanomaterials
Citation
Nanomaterials, 2016, v. 6, p. 61 How to Cite?
AbstractScutellaria baicalensis (SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticleencapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB) and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB) as the marker. The mucosal diffusion assay on Nano-SB was undertaken in a porcine model. The antibacterial effects of the mixed nanoparticles (Nano-MIX) of Nano-SB and Nano-CHX at 9:1 (w/w) ratio were analyzed in both planktonic and biofilm modes of representative oral bacteria. The Nano-MIX was effective on the mono-species biofilms of Streptococcus (S.) mutans, S. sobrinus, Fusobacterium (F.) nucleatum, and Aggregatibacter (A.) actinomycetemcomitans (MIC 50 μg/mL) at 24 h, and exhibited an enhanced effect against the multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans, and Porphyromonas (P.) gingivalis (MIC 12.5 μg/mL) at 24 h that was supported by the findings of both scanning electron microscopy (SEM) and confocal scanning laser microscopy (CLSM). This study shows enhanced synergistic antibacterial effects of the Nano-MIX on common oral bacterial biofilms, which could be potentially developed as a novel antimicrobial agent for clinical oral/periodontal care.
Persistent Identifierhttp://hdl.handle.net/10722/225611
ISSN
2018 Impact Factor: 4.034
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, KCF-
dc.contributor.authorSeneviratne, CJ-
dc.contributor.authorLi, X-
dc.contributor.authorLeung, PC-
dc.contributor.authorLau, CBS-
dc.contributor.authorWong, CH-
dc.contributor.authorPang, KY-
dc.contributor.authorWong, CW-
dc.contributor.authorWat, E-
dc.contributor.authorJin, L-
dc.date.accessioned2016-05-20T08:09:20Z-
dc.date.available2016-05-20T08:09:20Z-
dc.date.issued2016-
dc.identifier.citationNanomaterials, 2016, v. 6, p. 61-
dc.identifier.issn2079-4991-
dc.identifier.urihttp://hdl.handle.net/10722/225611-
dc.description.abstractScutellaria baicalensis (SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticleencapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB) and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB) as the marker. The mucosal diffusion assay on Nano-SB was undertaken in a porcine model. The antibacterial effects of the mixed nanoparticles (Nano-MIX) of Nano-SB and Nano-CHX at 9:1 (w/w) ratio were analyzed in both planktonic and biofilm modes of representative oral bacteria. The Nano-MIX was effective on the mono-species biofilms of Streptococcus (S.) mutans, S. sobrinus, Fusobacterium (F.) nucleatum, and Aggregatibacter (A.) actinomycetemcomitans (MIC 50 μg/mL) at 24 h, and exhibited an enhanced effect against the multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans, and Porphyromonas (P.) gingivalis (MIC 12.5 μg/mL) at 24 h that was supported by the findings of both scanning electron microscopy (SEM) and confocal scanning laser microscopy (CLSM). This study shows enhanced synergistic antibacterial effects of the Nano-MIX on common oral bacterial biofilms, which could be potentially developed as a novel antimicrobial agent for clinical oral/periodontal care.-
dc.languageeng-
dc.publisherMDPIAG. The Journal's web site is located at http://www.mdpi.com/journal/nanomaterials-
dc.relation.ispartofNanomaterials-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectAntimicrobials-
dc.subjectChlorhexidine-
dc.subjectNanotechnology-
dc.subjectOral biofilms-
dc.subjectScutellaria baicalensis-
dc.titleSynergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms-
dc.typeArticle-
dc.identifier.emailPang, KY: pangky@HKUCC-COM.hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.authorityJin, L=rp00028-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/nano6040061-
dc.identifier.scopuseid_2-s2.0-84964053762-
dc.identifier.hkuros258078-
dc.identifier.volume6-
dc.identifier.spage61-
dc.identifier.epage61-
dc.identifier.isiWOS:000375208800007-
dc.publisher.placeSwitzerland-

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