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postgraduate thesis: Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice
Title | Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice |
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Authors | |
Issue Date | 2015 |
Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
Citation | Chau, C. [周志強]. (2015). Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5617458 |
Abstract | Obesity is a global health problem experienced with various kinds of metabolic syndromes such as insulin resistance, hyperlipidemia and hypoadiponectinemia. To ameliorate obesity related problems, attention has been drawn to explore the role of adiponectin in obesity related metabolic dysregulation such that it can be selected as a therapeutic target to improve obesity related health problems. There are tools to over-express adiponectin such as drug induced secretion and osmotic pump drug delivery system for basic research. In this project, recombinant adeno-associated virus serotype 2/8 (rAAV2/8-LSP-mADN) was used to overexpress adiponectin in the liver of mouse for basic and translational research studies. Western blot of hepatocyte lysate and serum against adiponectin as well as hepatocyte viral quantitative PCR data suggests successful hepatic transduction. Although the expression level of adiponectin by this system need to further optimize, rAAV2/8-LSP-mADN is a valuable tool to over-express adiponectin in vivo safely and effectively. |
Degree | Master of Medical Sciences |
Subject | Adipose tissues Protein hormones Adenoviruses |
Dept/Program | Medicine |
Persistent Identifier | http://hdl.handle.net/10722/221512 |
HKU Library Item ID | b5617458 |
DC Field | Value | Language |
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dc.contributor.author | Chau, Chi-keung | - |
dc.contributor.author | 周志強 | - |
dc.date.accessioned | 2015-11-26T23:38:31Z | - |
dc.date.available | 2015-11-26T23:38:31Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Chau, C. [周志強]. (2015). Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5617458 | - |
dc.identifier.uri | http://hdl.handle.net/10722/221512 | - |
dc.description.abstract | Obesity is a global health problem experienced with various kinds of metabolic syndromes such as insulin resistance, hyperlipidemia and hypoadiponectinemia. To ameliorate obesity related problems, attention has been drawn to explore the role of adiponectin in obesity related metabolic dysregulation such that it can be selected as a therapeutic target to improve obesity related health problems. There are tools to over-express adiponectin such as drug induced secretion and osmotic pump drug delivery system for basic research. In this project, recombinant adeno-associated virus serotype 2/8 (rAAV2/8-LSP-mADN) was used to overexpress adiponectin in the liver of mouse for basic and translational research studies. Western blot of hepatocyte lysate and serum against adiponectin as well as hepatocyte viral quantitative PCR data suggests successful hepatic transduction. Although the expression level of adiponectin by this system need to further optimize, rAAV2/8-LSP-mADN is a valuable tool to over-express adiponectin in vivo safely and effectively. | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong (Pokfulam, Hong Kong) | - |
dc.relation.ispartof | HKU Theses Online (HKUTO) | - |
dc.rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject.lcsh | Adipose tissues | - |
dc.subject.lcsh | Protein hormones | - |
dc.subject.lcsh | Adenoviruses | - |
dc.title | Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice | - |
dc.type | PG_Thesis | - |
dc.identifier.hkul | b5617458 | - |
dc.description.thesisname | Master of Medical Sciences | - |
dc.description.thesislevel | Master | - |
dc.description.thesisdiscipline | Medicine | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.5353/th_b5617458 | - |
dc.identifier.mmsid | 991014569579703414 | - |