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Article: Photoaffinity labeling of transcription factors by DNA-templated crosslinking
Title | Photoaffinity labeling of transcription factors by DNA-templated crosslinking |
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Authors | |
Issue Date | 2015 |
Publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp |
Citation | Chemical Science, 2015, v. 6 n. 1, p. 745-751 How to Cite? |
Abstract | Characterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the protein-DNA interactions. We report a dual-probe method able to capture DNA-binding transcription factors with unmodified protein-binding sites in scenarios where conventional probes have failed. We have also shown the method's converse application in selecting specific transcription factor-binding DNA sequences from a probe library and its extension to studying proteins recognizing epigenetic marks. This study may provide a new tool for exploring DNA-binding proteins in biology. This journal is © The Royal Society of Chemistry. |
Persistent Identifier | http://hdl.handle.net/10722/221112 |
ISSN | 2023 Impact Factor: 7.6 2023 SCImago Journal Rankings: 2.333 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zheng, W | - |
dc.contributor.author | Zhang, W | - |
dc.contributor.author | Chen, N | - |
dc.contributor.author | Liu, Y | - |
dc.contributor.author | Liu, Y | - |
dc.contributor.author | Chen, L | - |
dc.contributor.author | Zhou, X | - |
dc.contributor.author | Chen, X | - |
dc.contributor.author | Zheng, H | - |
dc.contributor.author | Li, X | - |
dc.date.accessioned | 2015-10-27T07:11:10Z | - |
dc.date.available | 2015-10-27T07:11:10Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Chemical Science, 2015, v. 6 n. 1, p. 745-751 | - |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.uri | http://hdl.handle.net/10722/221112 | - |
dc.description.abstract | Characterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the protein-DNA interactions. We report a dual-probe method able to capture DNA-binding transcription factors with unmodified protein-binding sites in scenarios where conventional probes have failed. We have also shown the method's converse application in selecting specific transcription factor-binding DNA sequences from a probe library and its extension to studying proteins recognizing epigenetic marks. This study may provide a new tool for exploring DNA-binding proteins in biology. This journal is © The Royal Society of Chemistry. | - |
dc.language | eng | - |
dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp | - |
dc.relation.ispartof | Chemical Science | - |
dc.title | Photoaffinity labeling of transcription factors by DNA-templated crosslinking | - |
dc.type | Article | - |
dc.identifier.email | Li, X: xiaoyuli@hku.hk | - |
dc.identifier.authority | Li, X=rp02080 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1039/C4SC01953A | - |
dc.identifier.pmcid | PMC5494549 | - |
dc.identifier.scopus | eid_2-s2.0-84919344050 | - |
dc.identifier.hkuros | 274760 | - |
dc.identifier.volume | 6 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 745 | - |
dc.identifier.epage | 751 | - |
dc.identifier.isi | WOS:000345901600089 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2041-6520 | - |