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Conference Paper: Use of chondroitin sulphate lyases in scar treatment: kinetic considerations of macromolecular crowding
Title | Use of chondroitin sulphate lyases in scar treatment: kinetic considerations of macromolecular crowding |
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Authors | |
Issue Date | 2015 |
Citation | The 45th World Chemistry Congress (IUPAC-2015), Busan, Korea, 9-14 August 2015. How to Cite? |
Abstract | Chondroitin sulphate lyases ABC (ChABCs) have been exploited to digest upregulated chondroitin sulphate in nerve injury and disc herniation. The decay of enzyme activity under in vivo conditions remains an issue. In vitro, ChABCs showed activity decline by 60% to 70% at 37 oC in 30 min. By supplementation with Ficoll 400, the ChABC activity was sustainable for at least the 30-min assay period. Time-resolved UV absorption spectroscopy indicated the stabilisation of protein conformation under Ficoll supplementation at 200 mg/ml. The crowdedness led to lowering the Vmax and Km of the enzyme. At chondroitin sulphate C concentration as high as 1 mg/ml, enzyme activity was maximal when Ficoll supplementation was at 50 mg/ml for exolyase or 100 mg/ml for endolyase. Our data offer macromolecular crowding as means to extend the half-life of the lyases. |
Description | Conference Theme: Smart Chemistry, Better Life Session - Chemistry of Life (CL): no. CL-P1820-THU |
Persistent Identifier | http://hdl.handle.net/10722/218684 |
DC Field | Value | Language |
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dc.contributor.author | Tam, KW | - |
dc.contributor.author | Chan, YS | - |
dc.contributor.author | Shum, DKY | - |
dc.date.accessioned | 2015-09-18T06:50:11Z | - |
dc.date.available | 2015-09-18T06:50:11Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The 45th World Chemistry Congress (IUPAC-2015), Busan, Korea, 9-14 August 2015. | - |
dc.identifier.uri | http://hdl.handle.net/10722/218684 | - |
dc.description | Conference Theme: Smart Chemistry, Better Life | - |
dc.description | Session - Chemistry of Life (CL): no. CL-P1820-THU | - |
dc.description.abstract | Chondroitin sulphate lyases ABC (ChABCs) have been exploited to digest upregulated chondroitin sulphate in nerve injury and disc herniation. The decay of enzyme activity under in vivo conditions remains an issue. In vitro, ChABCs showed activity decline by 60% to 70% at 37 oC in 30 min. By supplementation with Ficoll 400, the ChABC activity was sustainable for at least the 30-min assay period. Time-resolved UV absorption spectroscopy indicated the stabilisation of protein conformation under Ficoll supplementation at 200 mg/ml. The crowdedness led to lowering the Vmax and Km of the enzyme. At chondroitin sulphate C concentration as high as 1 mg/ml, enzyme activity was maximal when Ficoll supplementation was at 50 mg/ml for exolyase or 100 mg/ml for endolyase. Our data offer macromolecular crowding as means to extend the half-life of the lyases. | - |
dc.language | eng | - |
dc.relation.ispartof | Proceedings of the 45th IUPAC World Chemistry Congress | - |
dc.title | Use of chondroitin sulphate lyases in scar treatment: kinetic considerations of macromolecular crowding | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Tam, KW: tamkw@hku.hk | - |
dc.identifier.email | Chan, YS: yschan@hku.hk | - |
dc.identifier.email | Shum, DKY: shumdkhk@hkucc.hku.hk | - |
dc.identifier.authority | Chan, YS=rp00318 | - |
dc.identifier.authority | Shum, DKY=rp00321 | - |
dc.identifier.hkuros | 251225 | - |
dc.identifier.hkuros | 267049 | - |