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Book Chapter: Generation and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease

TitleGeneration and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease
Authors
Lee, YKRan, XLai, KWHLau, VYMSiu, DCWTse, HF
KeywordsHuman induced pluripotent stem cell (hiPSC)
Retroviral reprogramming
Stem cell characterization
Cardiac differentiation
Cardiovascular disease modeling
Issue Date2016
PublisherHumana Press; Springer
Citation
Generation and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease. In Andras Nagy & Kursad Turksen (Eds.), Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization, p. 191-213. New York: Humana Press; Springer, 2016 How to Cite?
DOI: http://dx.doi.org/10.1007/7651_2015_273
AbstractAdvances in differentiation of cardiomyocytes from human induced pluripotent stem cell (hiPSC) was emerged as a tool for modeling of cardiovascular disease that recapitulate the phenotype for the purpose of drug screening, biomarkers discovery and testing of single nucleotide polymorphism (SNP) as a modifier for disease stratification. Here, we will describe the 1) retroviral reprogramming strategies in generation of human iPSC, 2) methodology in characterization of iPSC in order to identify the stem cell clones with the best quality; and 3) protocol of cardiac differentiation by modulation of Wnt signaling and β-catenin pathway.
Persistent Identifierhttp://hdl.handle.net/10722/215884
ISBN
9781493930333
ISSN
1064-3745
2023 SCImago Journal Rankings: 0.399
Series/Report no.Methods in Molecular Biology ; v. 1353
Springer protocols (Series)

 

DC FieldValueLanguage
dc.contributor.authorLee, YK-
dc.contributor.authorRan, X-
dc.contributor.authorLai, KWH-
dc.contributor.authorLau, VYM-
dc.contributor.authorSiu, DCW-
dc.contributor.authorTse, HF-
dc.date.accessioned2015-08-21T13:43:22Z-
dc.date.available2015-08-21T13:43:22Z-
dc.date.issued2016-
dc.identifier.citationGeneration and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease. In Andras Nagy & Kursad Turksen (Eds.), Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization, p. 191-213. New York: Humana Press; Springer, 2016-
dc.identifier.isbn9781493930333-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/215884-
dc.description.abstractAdvances in differentiation of cardiomyocytes from human induced pluripotent stem cell (hiPSC) was emerged as a tool for modeling of cardiovascular disease that recapitulate the phenotype for the purpose of drug screening, biomarkers discovery and testing of single nucleotide polymorphism (SNP) as a modifier for disease stratification. Here, we will describe the 1) retroviral reprogramming strategies in generation of human iPSC, 2) methodology in characterization of iPSC in order to identify the stem cell clones with the best quality; and 3) protocol of cardiac differentiation by modulation of Wnt signaling and β-catenin pathway.-
dc.languageeng-
dc.publisherHumana Press; Springer-
dc.relation.ispartofPatient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization-
dc.relation.ispartofseriesMethods in Molecular Biology ; v. 1353-
dc.relation.ispartofseriesSpringer protocols (Series)-
dc.subjectHuman induced pluripotent stem cell (hiPSC)-
dc.subjectRetroviral reprogramming-
dc.subjectStem cell characterization-
dc.subjectCardiac differentiation-
dc.subjectCardiovascular disease modeling-
dc.titleGeneration and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease-
dc.typeBook_Chapter-
dc.identifier.emailLee, YK: carol801@hku.hk-
dc.identifier.emailRan, X: karen517@HKUCC-COM.hku.hk-
dc.identifier.emailLai, KWH: kwhlai@hku.hk-
dc.identifier.emailLau, VYM: vymlau@hku.hk-
dc.identifier.emailSiu, DCW: cwdsiu@hkucc.hku.hk-
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.authoritySiu, DCW=rp00534-
dc.identifier.authorityTse, HF=rp00428-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/7651_2015_273-
dc.identifier.pmid26126449-
dc.identifier.scopuseid_2-s2.0-84946763205-
dc.identifier.hkuros248367-
dc.identifier.hkuros246526-
dc.identifier.spage191-
dc.identifier.epage213-
dc.identifier.eissn1940-6029-
dc.publisher.placeNew York-
dc.identifier.issnl1064-3745-

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