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Article: Modulation of porcine β-defensins 1 and 2 upon individual and combined Fusarium toxin exposure in a swine jejunal epithelial cell line

TitleModulation of porcine β-defensins 1 and 2 upon individual and combined Fusarium toxin exposure in a swine jejunal epithelial cell line
Authors
Issue Date2013
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://aem.asm.org/
Citation
Applied and Environmental Microbiology, 2013, v. 79 n. 7, p. 2225-2232 How to Cite?
AbstractDefensins are small antimicrobial peptides (AMPs) that play an important role in the innate immune system of mammals. Since the effect of mycotoxin contamination of food and feed on the secretion of intestinal AMPs is poorly understood, the aim of this study was to elucidate the individual and combined effects of four common Fusarium toxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA), and fumonisin B1 (FB1), on the mRNA expression, protein secretion, and corresponding antimicrobial effects of porcine β-defensins 1 and 2 (pBD-1 and pBD-2) using a porcine jejunal epithelial cell line, IPEC-J2. In general, upregulation of pBD-1 and pBD-2 mRNA expression occurred following exposure to Fusarium toxins, individually and in mixtures (P < 0.05). However, no significant increase in secreted pBD-1 and pBD-2 protein levels was observed, as measured by enzyme-linked immunosorbent assay (ELISA). Supernatants from IPEC-J2 cells exposed to toxins, singly or in combination, however, possessed significantly less antimicrobial activity against Escherichia coli than untreated supernatants. When single toxins and two-toxin combinations were assessed, toxicity effects were shown to be nonadditive (including synergism, potentiation, and antagonism), suggesting interactive toxin effects when cells are exposed to mycotoxin combinations. The results show that Fusarium toxins, individually and in mixtures, activate distinct antimicrobial defense mechanisms possessing the potential to alter the intestinal microbiota through diminished antimicrobial effects. Moreover, by evaluating toxin mixtures, this improved understanding of toxin effects will enable more effective risk assessments for common mycotoxin combinations observed in contaminated food and feed.
Persistent Identifierhttp://hdl.handle.net/10722/215558
ISSN
2020 Impact Factor: 4.792
2020 SCImago Journal Rankings: 1.552
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWan, MLY-
dc.contributor.authorWoo, CSJ-
dc.contributor.authorAllen, KJ-
dc.contributor.authorTurner, PC-
dc.contributor.authorEl-Nezamy, HS-
dc.date.accessioned2015-08-21T13:30:06Z-
dc.date.available2015-08-21T13:30:06Z-
dc.date.issued2013-
dc.identifier.citationApplied and Environmental Microbiology, 2013, v. 79 n. 7, p. 2225-2232-
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10722/215558-
dc.description.abstractDefensins are small antimicrobial peptides (AMPs) that play an important role in the innate immune system of mammals. Since the effect of mycotoxin contamination of food and feed on the secretion of intestinal AMPs is poorly understood, the aim of this study was to elucidate the individual and combined effects of four common Fusarium toxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA), and fumonisin B1 (FB1), on the mRNA expression, protein secretion, and corresponding antimicrobial effects of porcine β-defensins 1 and 2 (pBD-1 and pBD-2) using a porcine jejunal epithelial cell line, IPEC-J2. In general, upregulation of pBD-1 and pBD-2 mRNA expression occurred following exposure to Fusarium toxins, individually and in mixtures (P < 0.05). However, no significant increase in secreted pBD-1 and pBD-2 protein levels was observed, as measured by enzyme-linked immunosorbent assay (ELISA). Supernatants from IPEC-J2 cells exposed to toxins, singly or in combination, however, possessed significantly less antimicrobial activity against Escherichia coli than untreated supernatants. When single toxins and two-toxin combinations were assessed, toxicity effects were shown to be nonadditive (including synergism, potentiation, and antagonism), suggesting interactive toxin effects when cells are exposed to mycotoxin combinations. The results show that Fusarium toxins, individually and in mixtures, activate distinct antimicrobial defense mechanisms possessing the potential to alter the intestinal microbiota through diminished antimicrobial effects. Moreover, by evaluating toxin mixtures, this improved understanding of toxin effects will enable more effective risk assessments for common mycotoxin combinations observed in contaminated food and feed.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://aem.asm.org/-
dc.relation.ispartofApplied and Environmental Microbiology-
dc.titleModulation of porcine β-defensins 1 and 2 upon individual and combined Fusarium toxin exposure in a swine jejunal epithelial cell line-
dc.typeArticle-
dc.identifier.emailWan, MLY: murphyly@connect.hku.hk-
dc.identifier.emailAllen, KJ: kevin.allen@ubc.ca-
dc.identifier.emailTurner, PC: pturner3@umd.edu-
dc.identifier.emailEl-Nezamy, HS: elnezami@hkucc.hku.hk-
dc.identifier.authorityEl-Nezamy, HS=rp00694-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/AEM.03277-12-
dc.identifier.pmid23354708-
dc.identifier.pmcidPMC3623212-
dc.identifier.scopuseid_2-s2.0-84875543124-
dc.identifier.hkuros248355-
dc.identifier.hkuros213415-
dc.identifier.hkuros248230-
dc.identifier.volume79-
dc.identifier.issue7-
dc.identifier.spage2225-
dc.identifier.epage2232-
dc.identifier.isiWOS:000316183500014-
dc.publisher.placeUnited States-
dc.identifier.issnl0099-2240-

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