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Article: Association of Heparin with Basic Fibroblast Growth Factor, Epidermal Growth Factor, and Constitutive Nitric Oxide Synthase on Healing of Gastric Ulcer in Rats
Title | Association of Heparin with Basic Fibroblast Growth Factor, Epidermal Growth Factor, and Constitutive Nitric Oxide Synthase on Healing of Gastric Ulcer in Rats |
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Authors | |
Issue Date | 1999 |
Publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org/ |
Citation | The Journal of Pharmacology and Experimental Therapeutics, 1999, v. 290 n. 2, p. 789-796 How to Cite? |
Abstract | The healing effect of heparin on gastric ulcer and its underlying mechanisms were studied. The influences of protamine on these effects were also investigated. Gastric ulcer was induced by acetic acid in rats. Heparin (100-1000 U/kg i.v.) was given once daily for 4 or 7 days. Ulcer area was measured; gastric mucosal regeneration, proliferation, and angiogenesis were determined by histological or immunohistochemical methods. Gastric mucosal basic fibroblast growth factor (bFGF) level was assessed by an enzyme-linked immunosorbent assay, and the mucosal epidermal growth factor (EGF) level and nitric oxide synthase (NOS) activity were measured by radioimmunoassay. The anticoagulant action of heparin was determined by the duration of bleeding time. The results showed that heparin given for 4 or 7 days significantly accelerated gastric ulcer healing in a dose-dependent manner. The three doses of heparin significantly stimulated mucosal regeneration and proliferation as well as angiogenesis but not the contraction of ulcer base. Similar effects were observed in gastric mucosal bFGF and EGF levels and constitutive NOS activity. Protamine not only abolished the anticoagulant action of heparin but also significantly potentiated its effects on ulcer healing, gastric mucosal proliferation, angiogenesis, and constitutive NOS activity. These findings indicate that heparin can accelerate gastric ulcer healing, which is associated with mucosal regeneration, proliferation, and angiogenesis. These actions are likely to be stimulated by bFGF, EGF, and constitutive NOS activity in the gastric mucosa. Protamine potentiates the ulcer-healing effect of heparin, which is probably acting through constitutive NOS activation. |
Persistent Identifier | http://hdl.handle.net/10722/211451 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.829 |
DC Field | Value | Language |
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dc.contributor.author | Li, Y | - |
dc.contributor.author | Wang, HY | - |
dc.contributor.author | Cho, CH | - |
dc.date.accessioned | 2015-07-14T04:10:32Z | - |
dc.date.available | 2015-07-14T04:10:32Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | The Journal of Pharmacology and Experimental Therapeutics, 1999, v. 290 n. 2, p. 789-796 | - |
dc.identifier.issn | 0022-3565 | - |
dc.identifier.uri | http://hdl.handle.net/10722/211451 | - |
dc.description.abstract | The healing effect of heparin on gastric ulcer and its underlying mechanisms were studied. The influences of protamine on these effects were also investigated. Gastric ulcer was induced by acetic acid in rats. Heparin (100-1000 U/kg i.v.) was given once daily for 4 or 7 days. Ulcer area was measured; gastric mucosal regeneration, proliferation, and angiogenesis were determined by histological or immunohistochemical methods. Gastric mucosal basic fibroblast growth factor (bFGF) level was assessed by an enzyme-linked immunosorbent assay, and the mucosal epidermal growth factor (EGF) level and nitric oxide synthase (NOS) activity were measured by radioimmunoassay. The anticoagulant action of heparin was determined by the duration of bleeding time. The results showed that heparin given for 4 or 7 days significantly accelerated gastric ulcer healing in a dose-dependent manner. The three doses of heparin significantly stimulated mucosal regeneration and proliferation as well as angiogenesis but not the contraction of ulcer base. Similar effects were observed in gastric mucosal bFGF and EGF levels and constitutive NOS activity. Protamine not only abolished the anticoagulant action of heparin but also significantly potentiated its effects on ulcer healing, gastric mucosal proliferation, angiogenesis, and constitutive NOS activity. These findings indicate that heparin can accelerate gastric ulcer healing, which is associated with mucosal regeneration, proliferation, and angiogenesis. These actions are likely to be stimulated by bFGF, EGF, and constitutive NOS activity in the gastric mucosa. Protamine potentiates the ulcer-healing effect of heparin, which is probably acting through constitutive NOS activation. | - |
dc.language | eng | - |
dc.publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org/ | - |
dc.relation.ispartof | The Journal of Pharmacology and Experimental Therapeutics | - |
dc.subject.mesh | Anti-Ulcer Agents - therapeutic use | - |
dc.subject.mesh | Anticoagulants - therapeutic use | - |
dc.subject.mesh | Epidermal Growth Factor - metabolism - physiology | - |
dc.subject.mesh | Fibroblast Growth Factor 2 - metabolism - physiology | - |
dc.subject.mesh | Heparin - therapeutic use | - |
dc.title | Association of Heparin with Basic Fibroblast Growth Factor, Epidermal Growth Factor, and Constitutive Nitric Oxide Synthase on Healing of Gastric Ulcer in Rats | - |
dc.type | Article | - |
dc.identifier.email | Cho, CH: chcho@hkusua.hku.hk | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.pmid | 10411593 | - |
dc.identifier.hkuros | 45656 | - |
dc.identifier.volume | 290 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 789 | - |
dc.identifier.epage | 796 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-3565 | - |