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- Publisher Website: 10.1091/mbc.E13-10-0598
- Scopus: eid_2-s2.0-84901207762
- PMID: 24648492
- WOS: WOS:000339650800009
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Article: Casein kinase 1delta functions at the centrosome and Golgi to promote ciliogenesis
Title | Casein kinase 1delta functions at the centrosome and Golgi to promote ciliogenesis |
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Authors | |
Keywords | A Kinase Anchor Proteins/biosynthesis/*metabolism Animals Binding Sites Carrier Proteins Casein Kinase Idelta/*antagonists & inhibitors/genetics Cell Cycle Proteins/metabolism Cell Line Centrosome/*metabolism Cilia/*metabolism Golgi Apparatus/*metabolism Humans Mice Mice, Knockout Microtubule-Associated Proteins/biosynthesis/*metabolism Microtubules/metabolism Nuclear Proteins/metabolism Protein Transport RNA Interference RNA, Small Interfering Retina/cytology Signal Transduction/genetics TRPP Cation Channels/metabolism Telomerase/genetics rab GTP-Binding Proteins/metabolism |
Issue Date | 2014 |
Citation | Molecular Biology of the Cell, 2014, v. 25 n. 10, p. 1629-1640 How to Cite? |
Abstract | Inhibition of casein kinase 1 delta (CK1delta) blocks primary ciliogenesis in human telomerase reverse transcriptase immortalized retinal pigmented epithelial and mouse inner medullary collecting duct cells-3. Mouse embryonic fibroblasts (MEFs) and retinal cells from Csnk1d (CK1delta)-null mice also exhibit ciliogenesis defects. CK1delta catalytic activity and centrosomal localization signal (CLS) are required to rescue cilia formation in MEFs(Csnk1d null). Furthermore, expression of a truncated derivative containing the CLS displaces full-length CK1delta from the centrosome and decreases ciliary length in control MEFs, suggesting that centrosomal CK1delta has a role in ciliogenesis. CK1delta inhibition also alters pericentrosomal or ciliary distribution of several proteins involved in ciliary transport, including Ras-like in rat brain-11A, Ras-like in rat brain-8A, centrosomal protein of 290 kDa, pericentriolar material protein 1, and polycystin-2, as well as the Golgi distribution of its binding partner, A-kinase anchor protein 450 (AKAP450). As reported for AKAP450, CK1delta was required for microtubule nucleation at the Golgi and maintenance of Golgi integrity. Overexpression of an AKAP450 fragment containing the CK1delta-binding site inhibits Golgi-derived microtubule nucleation, Golgi distribution of intraflagellar transport protein 20 homologue, and ciliogenesis. Our results suggest that CK1delta mediates primary ciliogenesis by multiple mechanisms, one involving its centrosomal function and another dependent on its interaction with AKAP450 at the Golgi, where it is important for maintaining Golgi organization and polarized trafficking of multiple factors that mediate ciliary transport. |
Persistent Identifier | http://hdl.handle.net/10722/208433 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.566 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Greer, YE | en_US |
dc.contributor.author | Westlake, CJ | en_US |
dc.contributor.author | Gao, B | en_US |
dc.contributor.author | Bharti, K | en_US |
dc.contributor.author | Shiba, Y | en_US |
dc.contributor.author | Xavier, CP | en_US |
dc.contributor.author | Pazour, GJ | en_US |
dc.contributor.author | Yang, Y | en_US |
dc.contributor.author | Rubin, JS | en_US |
dc.date.accessioned | 2015-03-11T03:00:51Z | - |
dc.date.available | 2015-03-11T03:00:51Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Molecular Biology of the Cell, 2014, v. 25 n. 10, p. 1629-1640 | en_US |
dc.identifier.issn | 1939-4586 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/208433 | - |
dc.description.abstract | Inhibition of casein kinase 1 delta (CK1delta) blocks primary ciliogenesis in human telomerase reverse transcriptase immortalized retinal pigmented epithelial and mouse inner medullary collecting duct cells-3. Mouse embryonic fibroblasts (MEFs) and retinal cells from Csnk1d (CK1delta)-null mice also exhibit ciliogenesis defects. CK1delta catalytic activity and centrosomal localization signal (CLS) are required to rescue cilia formation in MEFs(Csnk1d null). Furthermore, expression of a truncated derivative containing the CLS displaces full-length CK1delta from the centrosome and decreases ciliary length in control MEFs, suggesting that centrosomal CK1delta has a role in ciliogenesis. CK1delta inhibition also alters pericentrosomal or ciliary distribution of several proteins involved in ciliary transport, including Ras-like in rat brain-11A, Ras-like in rat brain-8A, centrosomal protein of 290 kDa, pericentriolar material protein 1, and polycystin-2, as well as the Golgi distribution of its binding partner, A-kinase anchor protein 450 (AKAP450). As reported for AKAP450, CK1delta was required for microtubule nucleation at the Golgi and maintenance of Golgi integrity. Overexpression of an AKAP450 fragment containing the CK1delta-binding site inhibits Golgi-derived microtubule nucleation, Golgi distribution of intraflagellar transport protein 20 homologue, and ciliogenesis. Our results suggest that CK1delta mediates primary ciliogenesis by multiple mechanisms, one involving its centrosomal function and another dependent on its interaction with AKAP450 at the Golgi, where it is important for maintaining Golgi organization and polarized trafficking of multiple factors that mediate ciliary transport. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Molecular Biology of the Cell | en_US |
dc.subject | A Kinase Anchor Proteins/biosynthesis/*metabolism | en_US |
dc.subject | Animals | en_US |
dc.subject | Binding Sites | en_US |
dc.subject | Carrier Proteins | en_US |
dc.subject | Casein Kinase Idelta/*antagonists & inhibitors/genetics | en_US |
dc.subject | Cell Cycle Proteins/metabolism | en_US |
dc.subject | Cell Line | en_US |
dc.subject | Centrosome/*metabolism | en_US |
dc.subject | Cilia/*metabolism | en_US |
dc.subject | Golgi Apparatus/*metabolism | en_US |
dc.subject | Humans | en_US |
dc.subject | Mice | en_US |
dc.subject | Mice, Knockout | en_US |
dc.subject | Microtubule-Associated Proteins/biosynthesis/*metabolism | en_US |
dc.subject | Microtubules/metabolism | en_US |
dc.subject | Nuclear Proteins/metabolism | en_US |
dc.subject | Protein Transport | en_US |
dc.subject | RNA Interference | en_US |
dc.subject | RNA, Small Interfering | en_US |
dc.subject | Retina/cytology | en_US |
dc.subject | Signal Transduction/genetics | en_US |
dc.subject | TRPP Cation Channels/metabolism | en_US |
dc.subject | Telomerase/genetics | en_US |
dc.subject | rab GTP-Binding Proteins/metabolism | en_US |
dc.title | Casein kinase 1delta functions at the centrosome and Golgi to promote ciliogenesis | en_US |
dc.type | Article | en_US |
dc.identifier.email | Gao, B: gaobo@hku.hk | en_US |
dc.identifier.authority | Gao, B=rp02012 | en_US |
dc.identifier.doi | 10.1091/mbc.E13-10-0598 | en_US |
dc.identifier.pmid | 24648492 | - |
dc.identifier.scopus | eid_2-s2.0-84901207762 | - |
dc.identifier.volume | 25 | en_US |
dc.identifier.issue | 10 | en_US |
dc.identifier.spage | 1629 | en_US |
dc.identifier.epage | 1640 | en_US |
dc.identifier.isi | WOS:000339650800009 | - |
dc.identifier.issnl | 1059-1524 | - |