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postgraduate thesis: Adipocyte fatty acid binding protein acts as a suppressor of autophagy contributing to foam cell formation
Title | Adipocyte fatty acid binding protein acts as a suppressor of autophagy contributing to foam cell formation |
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Authors | |
Issue Date | 2014 |
Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
Citation | Wong, T. [黃德緒]. (2014). Adipocyte fatty acid binding protein acts as a suppressor of autophagy contributing to foam cell formation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5304001 |
Abstract | Background and objectives:
Growing bodies of evidence demonstrate that adipocyte fatty acid binding protein (A-FABP) mediates the pathogenesis of atherosclerosis through its direct impacts on macrophages. Loss-of-function study was conducted by utilizing peritoneal macrophages derived from A-FABP knockout (KO) mice, to investigate the role of A-FABP in autophagy and macrophage foam cell formation.
Key findings:
1. No morphological changes between the peritoneal macrophages derived from A-FABP knockout (KO) or their wild-type (WT) littermates.
2. Foam cell formation was successfully induced by the treatment of acetylated low-density lipoproteins (LDL) in peritoneal macrophages derived from A-FABP WT and KO mice.
3. LDL treatment induces autophagy in peritoneal macrophages from both A-FABP WT and KO mice.
4. The extent of LDL-induced autophagy is reduced in peritoneal macrophages of WT mice and is accompanied by increased lipid droplet accumulation when compared with A-FABP KO mice.
Conclusions:
A-FABP is a suppressor of autophagy and contributes to the attenuation of cholesterol efflux, subsequently resulting in enhancement of lipid droplets accumulation in peritoneal macrophages. A-FABP mediates the formation of macrophage foam cell via the suppression of autophagy. The results suggest that A-FABP is a potential therapeutic target to suspend the progression of atherosclerosis and remit the atherosclerotic lesion. |
Degree | Master of Medical Sciences |
Subject | Fatty acid-binding proteins Autophagic vacuoles |
Dept/Program | Medicine |
Persistent Identifier | http://hdl.handle.net/10722/206502 |
HKU Library Item ID | b5304001 |
DC Field | Value | Language |
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dc.contributor.author | Wong, Tak-sui | - |
dc.contributor.author | 黃德緒 | - |
dc.date.accessioned | 2014-11-03T23:14:50Z | - |
dc.date.available | 2014-11-03T23:14:50Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Wong, T. [黃德緒]. (2014). Adipocyte fatty acid binding protein acts as a suppressor of autophagy contributing to foam cell formation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5304001 | - |
dc.identifier.uri | http://hdl.handle.net/10722/206502 | - |
dc.description.abstract | Background and objectives: Growing bodies of evidence demonstrate that adipocyte fatty acid binding protein (A-FABP) mediates the pathogenesis of atherosclerosis through its direct impacts on macrophages. Loss-of-function study was conducted by utilizing peritoneal macrophages derived from A-FABP knockout (KO) mice, to investigate the role of A-FABP in autophagy and macrophage foam cell formation. Key findings: 1. No morphological changes between the peritoneal macrophages derived from A-FABP knockout (KO) or their wild-type (WT) littermates. 2. Foam cell formation was successfully induced by the treatment of acetylated low-density lipoproteins (LDL) in peritoneal macrophages derived from A-FABP WT and KO mice. 3. LDL treatment induces autophagy in peritoneal macrophages from both A-FABP WT and KO mice. 4. The extent of LDL-induced autophagy is reduced in peritoneal macrophages of WT mice and is accompanied by increased lipid droplet accumulation when compared with A-FABP KO mice. Conclusions: A-FABP is a suppressor of autophagy and contributes to the attenuation of cholesterol efflux, subsequently resulting in enhancement of lipid droplets accumulation in peritoneal macrophages. A-FABP mediates the formation of macrophage foam cell via the suppression of autophagy. The results suggest that A-FABP is a potential therapeutic target to suspend the progression of atherosclerosis and remit the atherosclerotic lesion. | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong (Pokfulam, Hong Kong) | - |
dc.relation.ispartof | HKU Theses Online (HKUTO) | - |
dc.rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject.lcsh | Fatty acid-binding proteins | - |
dc.subject.lcsh | Autophagic vacuoles | - |
dc.title | Adipocyte fatty acid binding protein acts as a suppressor of autophagy contributing to foam cell formation | - |
dc.type | PG_Thesis | - |
dc.identifier.hkul | b5304001 | - |
dc.description.thesisname | Master of Medical Sciences | - |
dc.description.thesislevel | Master | - |
dc.description.thesisdiscipline | Medicine | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.5353/th_b5304001 | - |
dc.identifier.mmsid | 991039639009703414 | - |