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Conference Paper: Self-Renewal of a de Novo Population of C-Kit+ Cells Confers a Sustainable Growth of Neuroblastoma
Title | Self-Renewal of a de Novo Population of C-Kit+ Cells Confers a Sustainable Growth of Neuroblastoma |
---|---|
Authors | |
Issue Date | 2014 |
Publisher | The International Society for Stem Cell Research (ISSCR). |
Citation | The 12th Annual Meeting of the International Society for Stem Cell Research (ISSCR 2014), Vancouver, Canada, 18-21 June 2014. In Poster Abstracts, 2014, p. 209, abstract no. T-1108 How to Cite? |
Abstract | High cellular heterogeneity within neuroblastomas (NB) may
account for the non-uniform response to treatment. c-KIT+ cells
are frequently detected in NB, but how they influence NB behavior
still remains elusive. Here, we used NB tumor initiating cells to
reconstitute NB development and demonstrated that c-KIT+ cells are
de novo generated and dynamically maintained within the tumors to
sustain tumor progression. c-KIT+ NB cells express higher levels of
neural crest and stem cell markers (SLUG, SOX2, NANOG) and are
endowed with high clonogenic capacity, differentiation plasticity and
are refractory to drugs. With serial transplantation assays, we found
that c-KIT expression is not required for tumor formation, but c-KIT+
cells are more aggressive and can induce tumors 9-fold more efficiently
than c-KIT-/low cells. Intriguingly, c-KIT+ cells exhibited a long-term
in vivo self-renewal capacity to sustain the formation of secondary
and tertiary tumors in mice. In addition, we showed that Prokineticin
signaling and MAPK pathways are crucial for the maintenance of
c-KIT+ cells in tumor to promote NB progression. Our results highlight
the importance of this de novo population of NB cells in sustainable
growth of NB and reveal specific signalling pathways that may provide targets leading to more effective NB therapies. |
Description | Poster Presentation - Session: Cancer Cell |
Persistent Identifier | http://hdl.handle.net/10722/204494 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lau, CST | en_US |
dc.contributor.author | Hansford, LM | en_US |
dc.contributor.author | Kaplan, DR | en_US |
dc.contributor.author | Ngan, ESW | en_US |
dc.date.accessioned | 2014-09-19T23:57:03Z | - |
dc.date.available | 2014-09-19T23:57:03Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | The 12th Annual Meeting of the International Society for Stem Cell Research (ISSCR 2014), Vancouver, Canada, 18-21 June 2014. In Poster Abstracts, 2014, p. 209, abstract no. T-1108 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/204494 | - |
dc.description | Poster Presentation - Session: Cancer Cell | - |
dc.description.abstract | High cellular heterogeneity within neuroblastomas (NB) may account for the non-uniform response to treatment. c-KIT+ cells are frequently detected in NB, but how they influence NB behavior still remains elusive. Here, we used NB tumor initiating cells to reconstitute NB development and demonstrated that c-KIT+ cells are de novo generated and dynamically maintained within the tumors to sustain tumor progression. c-KIT+ NB cells express higher levels of neural crest and stem cell markers (SLUG, SOX2, NANOG) and are endowed with high clonogenic capacity, differentiation plasticity and are refractory to drugs. With serial transplantation assays, we found that c-KIT expression is not required for tumor formation, but c-KIT+ cells are more aggressive and can induce tumors 9-fold more efficiently than c-KIT-/low cells. Intriguingly, c-KIT+ cells exhibited a long-term in vivo self-renewal capacity to sustain the formation of secondary and tertiary tumors in mice. In addition, we showed that Prokineticin signaling and MAPK pathways are crucial for the maintenance of c-KIT+ cells in tumor to promote NB progression. Our results highlight the importance of this de novo population of NB cells in sustainable growth of NB and reveal specific signalling pathways that may provide targets leading to more effective NB therapies. | - |
dc.language | eng | en_US |
dc.publisher | The International Society for Stem Cell Research (ISSCR). | - |
dc.relation.ispartof | Annual Meeting of the International Society for Stem Cell Research, ISSCR 2014 | en_US |
dc.title | Self-Renewal of a de Novo Population of C-Kit+ Cells Confers a Sustainable Growth of Neuroblastoma | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Lau, CST: cynlau@hku.hk | en_US |
dc.identifier.email | Ngan, ESW: engan@hku.hk | en_US |
dc.identifier.authority | Ngan, ESW=rp00422 | en_US |
dc.identifier.hkuros | 240134 | en_US |
dc.identifier.spage | 209, abstract no. T-1108 | - |
dc.identifier.epage | 209, abstract no. T-1108 | - |
dc.publisher.place | United States | - |