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Article: Evaluation of real-time PCR for quantitative detection of Escherichia coli in beach water

TitleEvaluation of real-time PCR for quantitative detection of Escherichia coli in beach water
Authors
Keywordscyd
Escherichia coli
Hybridization probes
Marine beach water
Real-time PCR
Issue Date2014
PublisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwaterhealth
Citation
Journal of Water and Health, 2014, v. 12 n. 1, p. 51-56 How to Cite?
AbstractThe current investigation evaluated the use of real-time polymerase chain reaction (PCR) for quantitative detection of Escherichia coli in marine beach water. Densities of E. coli in 263 beach water samples collected from 13 bathing beaches in Hong Kong between November 2008 and December 2009 were determined using both real-time PCR and culture-based methods. Regression analysis showed that these two methods had a significant positive linear relationship with a correlation coefficient (r) of 0.64. Serial dilution of spiked samples indicated that the real-time PCR had a limit of quantification of 25 E. coli colonies in 100 mL water sample. This study showed that the rapid real-time PCR has potential to complement the traditional culture method of assessing fecal pollution in marine beach water.
Persistent Identifierhttp://hdl.handle.net/10722/203188
ISSN
2023 Impact Factor: 2.5
2023 SCImago Journal Rankings: 0.534
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLam, JTH-
dc.contributor.authorLui, E-
dc.contributor.authorChau, S-
dc.contributor.authorKueh, CSW-
dc.contributor.authorYung, YK-
dc.contributor.authorYam, WC-
dc.date.accessioned2014-09-19T12:56:57Z-
dc.date.available2014-09-19T12:56:57Z-
dc.date.issued2014-
dc.identifier.citationJournal of Water and Health, 2014, v. 12 n. 1, p. 51-56-
dc.identifier.issn1477-8920-
dc.identifier.urihttp://hdl.handle.net/10722/203188-
dc.description.abstractThe current investigation evaluated the use of real-time polymerase chain reaction (PCR) for quantitative detection of Escherichia coli in marine beach water. Densities of E. coli in 263 beach water samples collected from 13 bathing beaches in Hong Kong between November 2008 and December 2009 were determined using both real-time PCR and culture-based methods. Regression analysis showed that these two methods had a significant positive linear relationship with a correlation coefficient (r) of 0.64. Serial dilution of spiked samples indicated that the real-time PCR had a limit of quantification of 25 E. coli colonies in 100 mL water sample. This study showed that the rapid real-time PCR has potential to complement the traditional culture method of assessing fecal pollution in marine beach water.-
dc.languageeng-
dc.publisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwaterhealth-
dc.relation.ispartofJournal of Water and Health-
dc.rightsJournal of Water and Health. Copyright © I W A Publishing.-
dc.rights©IWA Publishing [year of publication]. The definitive peer-reviewed and edited version of this article is published in [name of journal] [volume] [issue] [pages] [year] [DOI] and is available at www.iwapublishing.com-
dc.subjectcyd-
dc.subjectEscherichia coli-
dc.subjectHybridization probes-
dc.subjectMarine beach water-
dc.subjectReal-time PCR-
dc.titleEvaluation of real-time PCR for quantitative detection of Escherichia coli in beach water-
dc.typeArticle-
dc.identifier.emailLam, JTH: jasonlam@graduate.hku.hk-
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hk-
dc.identifier.authorityYam, WC=rp00313-
dc.identifier.doi10.2166/wh.2013.038-
dc.identifier.pmid24642432-
dc.identifier.scopuseid_2-s2.0-84898748670-
dc.identifier.hkuros240440-
dc.identifier.volume12-
dc.identifier.issue1-
dc.identifier.spage51-
dc.identifier.epage56-
dc.identifier.isiWOS:000338511500006-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1477-8920-

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