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Article: Probing the coupled adhesion and deformation characteristics of suspension cells

TitleProbing the coupled adhesion and deformation characteristics of suspension cells
Authors
Issue Date2014
PublisherAmerican Institute of Physics. The Journal's web site is located at http://apl.aip.org/
Citation
Applied Physics Letters, 2014, v. 105 n. 7, article no. 073703, p. 1-5 How to Cite?
AbstractBy combining optical trapping with fluorescence imaging, the adhesion and deformation characteristics of suspension cells were probed on single cell level. We found that, after 24 h of co-culturing, stable attachment between non-adherent K562 cells and polystyrene beads coated with fibronectin, collagen I, or G-actin can all be formed with an adhesion energy density in the range of 1–3×10−2 mJ/m2, which is about one order of magnitude lower than the reported values for several adherent cells. In addition, it was observed that the formation of a stronger adhesion is accompanied with the appearance of a denser actin cell cortex, especially in the region close to the cell-bead interface, resulting in a significant increase in the apparent modulus of the cell. Findings here could be important for our understanding of why the aggregation of circulating cells, like that in leukostasis, takes place in vivo as well as how such clusters of non-adherent cells behave. The method proposed can also be useful in investigating adhesion and related phenomena for other cell types in the future. This work was supported by a grant from the Research Grants Council (Project No. HKU 7143/12E) of the Hong Kong Special Administration Region as well as a seed fund (Project No. 201211159001) from The University of Hong Kong. J.Q. acknowledges support from the National Natural Science Foundation of China (Nos. 11202184 and 11321202).
Persistent Identifierhttp://hdl.handle.net/10722/203035
ISSN
2019 Impact Factor: 3.597
2015 SCImago Journal Rankings: 1.105
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHui, THen_US
dc.contributor.authorZhu, Qen_US
dc.contributor.authorZhou, ZLen_US
dc.contributor.authorQian, Jen_US
dc.contributor.authorLin, Yen_US
dc.date.accessioned2014-09-19T11:08:13Z-
dc.date.available2014-09-19T11:08:13Z-
dc.date.issued2014en_US
dc.identifier.citationApplied Physics Letters, 2014, v. 105 n. 7, article no. 073703, p. 1-5-
dc.identifier.issn0003-6951-
dc.identifier.urihttp://hdl.handle.net/10722/203035-
dc.description.abstractBy combining optical trapping with fluorescence imaging, the adhesion and deformation characteristics of suspension cells were probed on single cell level. We found that, after 24 h of co-culturing, stable attachment between non-adherent K562 cells and polystyrene beads coated with fibronectin, collagen I, or G-actin can all be formed with an adhesion energy density in the range of 1–3×10−2 mJ/m2, which is about one order of magnitude lower than the reported values for several adherent cells. In addition, it was observed that the formation of a stronger adhesion is accompanied with the appearance of a denser actin cell cortex, especially in the region close to the cell-bead interface, resulting in a significant increase in the apparent modulus of the cell. Findings here could be important for our understanding of why the aggregation of circulating cells, like that in leukostasis, takes place in vivo as well as how such clusters of non-adherent cells behave. The method proposed can also be useful in investigating adhesion and related phenomena for other cell types in the future. This work was supported by a grant from the Research Grants Council (Project No. HKU 7143/12E) of the Hong Kong Special Administration Region as well as a seed fund (Project No. 201211159001) from The University of Hong Kong. J.Q. acknowledges support from the National Natural Science Foundation of China (Nos. 11202184 and 11321202).-
dc.languageengen_US
dc.publisherAmerican Institute of Physics. The Journal's web site is located at http://apl.aip.org/-
dc.relation.ispartofApplied Physics Lettersen_US
dc.rightsCopyright 2014 AIP Publishing LLC. This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Applied Physics Letters, 2014, v. 105 n. 7, article no. 073703, p. 1-5 and may be found at https://doi.org/10.1063/1.4893734-
dc.titleProbing the coupled adhesion and deformation characteristics of suspension cellsen_US
dc.typeArticleen_US
dc.identifier.emailLin, Y: ylin@hku.hken_US
dc.identifier.authorityLin, Y=rp00080en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1063/1.4893734-
dc.identifier.scopuseid_2-s2.0-84929439303-
dc.identifier.hkuros239621en_US
dc.identifier.volume105en_US
dc.identifier.issue7-
dc.identifier.spagearticle no. 073703, p. 1-
dc.identifier.epagearticle no. 073703, p. 5-
dc.identifier.isiWOS:000341189800105-
dc.publisher.placeUnited States-
dc.identifier.issnl0003-6951-

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