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- Publisher Website: 10.1128/JCM.37.5.1621-1624.1999
- Scopus: eid_2-s2.0-0032895585
- PMID: 10203541
- WOS: WOS:000079792400079
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Article: Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR
Title | Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR |
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Authors | |
Issue Date | 1999 |
Citation | Journal of Clinical Microbiology, 1999, v. 37, n. 5, p. 1621-1624 How to Cite? |
Abstract | In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation. |
Persistent Identifier | http://hdl.handle.net/10722/200070 |
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.653 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Conrads, Georg | - |
dc.contributor.author | Flemmig, Thomas Frank | - |
dc.contributor.author | Seyfarth, Ilse | - |
dc.contributor.author | Lampert, Fritz H. | - |
dc.contributor.author | Lütticken, Rudolf | - |
dc.date.accessioned | 2014-07-26T23:11:06Z | - |
dc.date.available | 2014-07-26T23:11:06Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Journal of Clinical Microbiology, 1999, v. 37, n. 5, p. 1621-1624 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | http://hdl.handle.net/10722/200070 | - |
dc.description.abstract | In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Clinical Microbiology | - |
dc.title | Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1128/JCM.37.5.1621-1624.1999 | - |
dc.identifier.pmid | 10203541 | - |
dc.identifier.scopus | eid_2-s2.0-0032895585 | - |
dc.identifier.volume | 37 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1621 | - |
dc.identifier.epage | 1624 | - |
dc.identifier.isi | WOS:000079792400079 | - |
dc.identifier.issnl | 0095-1137 | - |