File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1152/ajpheart.00766.2002
- Scopus: eid_2-s2.0-0037377574
- PMID: 12595286
- WOS: WOS:000181425900048
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Differential response of cardiac fibroblasts from young adult and senescent rats to ANG II
Title | Differential response of cardiac fibroblasts from young adult and senescent rats to ANG II |
---|---|
Authors | |
Keywords | Angiotensin type 1 receptor Cardiac fibrosis Collagen Renin-angiotensin system Transforming growth factor-β |
Issue Date | 2003 |
Citation | American Journal of Physiology - Heart and Circulatory Physiology, 2003, v. 284 n. 4 53-4, p. H1454-H1459 How to Cite? |
Abstract | The intracardiac ANG II-forming pathway is activated in the senescent myocardium, raising the possibility of enhanced ANG II effects on cardiac fibroblasts. This study established an in vitro model of cultured cardiac fibroblasts from aged rats to examine if the response of these cells to ANG II is modified in the aged heart. Levels of mRNA encoding renin, angiotensinogen, and the AT1 receptor subtype in cardiac fibroblasts from young adult and senescent rats were quantified by RT-PCR, net collagen production by a hydroxyproline-based assay, and transforming growth factor (TGF)-β levels using a commercial kit. In cardiac fibroblasts from young adult rats, ANG II significantly enhanced AT1 mRNA levels, net collagen production, and TGF-β production. In fibroblasts from the aged myocardium, ANG II downregulated AT1 mRNA expression, had a less pronounced effect on net collagen production, and had no effect on TGF-β production. Such age-related modification of the response of cardiac fibroblasts to ANG II may counteract the effects of augmented intracardiac ANG II production in the senescent heart, limiting fibrogenesis. |
Persistent Identifier | http://hdl.handle.net/10722/195165 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.452 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shivakumar, K | - |
dc.contributor.author | Dostal, DE | - |
dc.contributor.author | Boheler, K | - |
dc.contributor.author | Baker, KM | - |
dc.contributor.author | Lakatta, EG | - |
dc.date.accessioned | 2014-02-25T01:40:15Z | - |
dc.date.available | 2014-02-25T01:40:15Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | American Journal of Physiology - Heart and Circulatory Physiology, 2003, v. 284 n. 4 53-4, p. H1454-H1459 | - |
dc.identifier.issn | 0363-6135 | - |
dc.identifier.uri | http://hdl.handle.net/10722/195165 | - |
dc.description.abstract | The intracardiac ANG II-forming pathway is activated in the senescent myocardium, raising the possibility of enhanced ANG II effects on cardiac fibroblasts. This study established an in vitro model of cultured cardiac fibroblasts from aged rats to examine if the response of these cells to ANG II is modified in the aged heart. Levels of mRNA encoding renin, angiotensinogen, and the AT1 receptor subtype in cardiac fibroblasts from young adult and senescent rats were quantified by RT-PCR, net collagen production by a hydroxyproline-based assay, and transforming growth factor (TGF)-β levels using a commercial kit. In cardiac fibroblasts from young adult rats, ANG II significantly enhanced AT1 mRNA levels, net collagen production, and TGF-β production. In fibroblasts from the aged myocardium, ANG II downregulated AT1 mRNA expression, had a less pronounced effect on net collagen production, and had no effect on TGF-β production. Such age-related modification of the response of cardiac fibroblasts to ANG II may counteract the effects of augmented intracardiac ANG II production in the senescent heart, limiting fibrogenesis. | - |
dc.language | eng | - |
dc.relation.ispartof | American Journal of Physiology - Heart and Circulatory Physiology | - |
dc.subject | Angiotensin type 1 receptor | - |
dc.subject | Cardiac fibrosis | - |
dc.subject | Collagen | - |
dc.subject | Renin-angiotensin system | - |
dc.subject | Transforming growth factor-β | - |
dc.title | Differential response of cardiac fibroblasts from young adult and senescent rats to ANG II | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1152/ajpheart.00766.2002 | - |
dc.identifier.pmid | 12595286 | - |
dc.identifier.scopus | eid_2-s2.0-0037377574 | - |
dc.identifier.volume | 284 | - |
dc.identifier.issue | 4 53-4 | - |
dc.identifier.spage | H1454 | - |
dc.identifier.epage | H1459 | - |
dc.identifier.isi | WOS:000181425900048 | - |
dc.identifier.issnl | 0363-6135 | - |