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Conference Paper: A comparative study of cell-free Epstein-Barr virus DNA and microRNAs in nasopharyngeal carcinoma screening
Title | A comparative study of cell-free Epstein-Barr virus DNA and microRNAs in nasopharyngeal carcinoma screening |
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Authors | |
Keywords | Medical sciences Experimental medicine, laboratory technique |
Issue Date | 2010 |
Publisher | International Institute of Anticancer Research. The Journal's web site is located at iiar@iiar-anticancer.org |
Citation | The 4th International Congress of Molecular Medicine, Istanbul, Turkey, 27-30 June, 2011. In in vivo, 2011, v. 25 n. 3, p. 541, abstract no. 195 How to Cite? |
Abstract | BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignant squamous cell carcinoma in the head and neck region. Epstein-Barr virus (EBV) is associated with undifferentiated NPC. Cell-free EBV DNA is routinely used as a serological marker. Circulating nucleic acids were recently suggested as non-invasive diagnosis markers. This study aims to examine the use of microRNAs (miRNAs) as a supplement to existing screening methods. METHODS: The expression levels of hsa-miR-21, hsa-miR-1301, ebv-miRBART7 and ebv-miR-BART22 were investigated in ten paired tumor-normal tissues, plasmas of sixty other NPC patients and twenty-five cohorts. Plasmas from thirteen NPC patients were evaluated for both EBV and miRNAs levels between pre-operative and post-operative (three months after radiotherapy) patients. MiRNA and EBV DNA quantitation were performed by quantitative real-time PCR and EBV quant kit, respectively. RESULTS: MiR-BART7 showed higher expression in tumor tissues (p=0.038) and in NPC plasma samples with sensitivity 71.15% and specificity 76.47%. Among patients who had already received radiotherapy, 12 of 13 patients showed elevated miR-BART7 expression whereas 4 of 13 patients showed elevated EBV DNA, 3 of 13 patients had increased and 5 of 13 undetermined. CONCLUSION: MiR-BART7 showed significantly higher expression in NPC with good sensitivity and specificity. Further studies will be undertaken on the functional role of miR-BART7. |
Persistent Identifier | http://hdl.handle.net/10722/194800 |
ISSN | 2023 Impact Factor: 1.8 2023 SCImago Journal Rankings: 0.555 |
DC Field | Value | Language |
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dc.contributor.author | Man, OY | en_US |
dc.contributor.author | Wong, STS | en_US |
dc.contributor.author | Chan, YW | en_US |
dc.contributor.author | Wei, WI | en_US |
dc.date.accessioned | 2014-02-17T02:10:25Z | - |
dc.date.available | 2014-02-17T02:10:25Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.citation | The 4th International Congress of Molecular Medicine, Istanbul, Turkey, 27-30 June, 2011. In in vivo, 2011, v. 25 n. 3, p. 541, abstract no. 195 | en_US |
dc.identifier.issn | 0258-851X | - |
dc.identifier.uri | http://hdl.handle.net/10722/194800 | - |
dc.description.abstract | BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignant squamous cell carcinoma in the head and neck region. Epstein-Barr virus (EBV) is associated with undifferentiated NPC. Cell-free EBV DNA is routinely used as a serological marker. Circulating nucleic acids were recently suggested as non-invasive diagnosis markers. This study aims to examine the use of microRNAs (miRNAs) as a supplement to existing screening methods. METHODS: The expression levels of hsa-miR-21, hsa-miR-1301, ebv-miRBART7 and ebv-miR-BART22 were investigated in ten paired tumor-normal tissues, plasmas of sixty other NPC patients and twenty-five cohorts. Plasmas from thirteen NPC patients were evaluated for both EBV and miRNAs levels between pre-operative and post-operative (three months after radiotherapy) patients. MiRNA and EBV DNA quantitation were performed by quantitative real-time PCR and EBV quant kit, respectively. RESULTS: MiR-BART7 showed higher expression in tumor tissues (p=0.038) and in NPC plasma samples with sensitivity 71.15% and specificity 76.47%. Among patients who had already received radiotherapy, 12 of 13 patients showed elevated miR-BART7 expression whereas 4 of 13 patients showed elevated EBV DNA, 3 of 13 patients had increased and 5 of 13 undetermined. CONCLUSION: MiR-BART7 showed significantly higher expression in NPC with good sensitivity and specificity. Further studies will be undertaken on the functional role of miR-BART7. | - |
dc.language | eng | en_US |
dc.publisher | International Institute of Anticancer Research. The Journal's web site is located at iiar@iiar-anticancer.org | - |
dc.relation.ispartof | in vivo | en_US |
dc.subject | Medical sciences | - |
dc.subject | Experimental medicine, laboratory technique | - |
dc.title | A comparative study of cell-free Epstein-Barr virus DNA and microRNAs in nasopharyngeal carcinoma screening | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Wong, STS: thiansze@graduate.hku.hk | en_US |
dc.identifier.email | Chan, YW: jywchan1@hku.hk | en_US |
dc.identifier.email | Wei, WI: hrmswwi@hku.hk | en_US |
dc.identifier.authority | Wong, STS=rp00478 | en_US |
dc.identifier.authority | Chan, YW=rp01314 | en_US |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.hkuros | 227809 | en_US |
dc.identifier.volume | 25 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 541 | - |
dc.identifier.epage | 541 | - |
dc.publisher.place | Greece | - |
dc.identifier.issnl | 0258-851X | - |