Comparative study of nuclear organier regions (NORs) on chromosome of the cultured lymphocyte from nasopharyngeal carcinoma patients, IgA/VCA positive individuals and normal controls

Abstract

The frequences of silver-stained NORs and choromosome union of theperipheral lymphocytes from three groups (NPC group, Ig/A/VCA positive group and normal control group of persons), com pared, were The results showed that the average values of Ag-NORs were much higher in NPC group than those in other two groups (P<0.001)., and the number of chromosome union per case was also markedly higher in NPC group than those in other two groups (P<0.05 and P<0.01). There was no significant difference in the frequences of Ag-NORs and chromosome union between IgA/VCA positive group and normal control group (P>0.05).The results suggested that increase of quantity and transcribed activity of rRNA gene on chromosome of NPC patients may play some roles in the tumorigenesis of the tumor.

本文應用銀染技術對鼻咽癌患者,IgA/VCA陽性人群和正常人群淋巴細胞染色體的Ag—NOR s頻率和染色體聯合頻率進行比較研究。結果顯示鼻咽癌患者D組、G組與D+G組染色體的NORs平均值,與正常人群和IgA/VCA陽性人群比較,均明顯升高。經統計學處理,差異十分顯著(P值均<0.01)。此外,鼻咽癌組平均每例染色體聯合數目亦明顯高于正常對照組和IgA/VCA陽性組。統計學處理有顯著性差異,P值分別<0.05和<0.01。上兩項指標中,IgA/VCA陽性組與正常對照組比較均無明顯差異。本研究結果提示,鼻咽癌患者rRNA基因的數量和轉錄活性增強可能與腫瘤發生有一定的關系,本文對此也進行了簡短的討論。