Effects of 5F on IκKβ, IκB, p65, and p50 mRNA expression of non-small lung cancer NCI-H460 cells

Abstract

Objective: To investigate the inhibitory mechanism of 5F isolated from Pteris semipinnata on the proliferation and apoptosis induction of NCI-H460 cells. The roles of NF-κB pathway in the apoptosis were explored in this study. Methods: NCI-H460 Cell growth inhibition mediated by 5F was detected by MTT assay. RT-PCR was Applied to examing the mRNA levels of IκKβ, IκB, p65, and p50. Results: 5F Showed the growth inhibitory activity against NCI-H460 cells with IC50 values of 21.40, 4.52, and 1.02 μg/mL for 24, 48, and 72 h, respectively. The mRNA expression levels of IκKβ and IκB were significantly decreased after 5F treated cells for 6 h (P<0.05); The mRNA expression levels of p65 and p50 were significantly decreased after 5F treated cells for 3 h (P<0.05). Conclusion: The mechanism for 5F induction of NCI-H460 cells apoptosis could be mediated by the inhibition of NF-κB pathway.