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- Publisher Website: 10.1016/j.jep.2012.08.026
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- PMID: 22960543
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Article: Topical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in rats
Title | Topical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in rats |
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Authors | |
Keywords | Bone cancer pain Bone-specific alkaline phosphatase Chinese herbs Osteoclast Tong-Luo-San-Jie (TLSJ) gel Type I collagen carboxy-terminal telopeptide |
Issue Date | 2012 |
Publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm |
Citation | Journal Of Ethnopharmacology, 2012, v. 143 n. 3, p. 905-913 How to Cite? |
Abstract | Ethnopharmacological relevance: The herbal analgesic gel Tong-Luo-San-Jie (TLSJ) and its modifications are used in traditional Chinese medicine to manage cancer pain. However, its mechanisms are still unknown. Aim of the study: To investigate the effects and mechanisms of TLSJ gel on bone cancer pain in a rat model. Materials and methods: A bone cancer pain rat model was established by inoculating Walker 256 rat carcinoma cells directly into the right tibial medullary cavity of Sprague-Dawley rats (150-170 g); Phosphate buffered saline (PBS) tibial inoculation was used as control. Cancer-bearing rats were treated twice a day with external TLSJ gel (0.5 g/cm2/day) or inert gel control for 21 day (n=10/group). Behavioral tests such as mechanical threshold and paw withdrawal latency (PWL) were carried out. Osteoclastic activities were determined and carboxyterminal pyridinoline cross-linked type I collagen telopeptides (ICTP) and bone-specific alkaline phosphatase (BAP) concentrations were detected with ELISA after treatment. Adverse effects were monitored, and biochemical and histological tests were performed in naïve rats treated with local TLSJ gel for six weeks. Results: TLSJ treatment significantly restored bone cancer-induced decrease of PWL and mechanical threshold compared to inert gel. It also decreased the level of blood serum ICTP and BAP and inhibited osteoclast activities. No adverse effects or abnormal biochemical and histological changes were detected after TLSJ treatment. Conclusion: The present study shows that TLSJ significantly inhibits bone cancer-induced thermal and mechanical sensitization. It suggests that the gel may be useful in managing cancer pain and that it may act by inhibiting osteoclastic activity. © 2012 Elsevier Ireland Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/188656 |
ISSN | 2023 Impact Factor: 4.8 2023 SCImago Journal Rankings: 0.936 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, J | en_US |
dc.contributor.author | Zhang, R | en_US |
dc.contributor.author | Dong, C | en_US |
dc.contributor.author | Jiao, L | en_US |
dc.contributor.author | Xu, L | en_US |
dc.contributor.author | Liu, J | en_US |
dc.contributor.author | Wang, Z | en_US |
dc.contributor.author | Mao Ying, QL | en_US |
dc.contributor.author | Fong, H | en_US |
dc.contributor.author | Lao, L | en_US |
dc.date.accessioned | 2013-09-03T04:10:53Z | - |
dc.date.available | 2013-09-03T04:10:53Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Journal Of Ethnopharmacology, 2012, v. 143 n. 3, p. 905-913 | en_US |
dc.identifier.issn | 0378-8741 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/188656 | - |
dc.description.abstract | Ethnopharmacological relevance: The herbal analgesic gel Tong-Luo-San-Jie (TLSJ) and its modifications are used in traditional Chinese medicine to manage cancer pain. However, its mechanisms are still unknown. Aim of the study: To investigate the effects and mechanisms of TLSJ gel on bone cancer pain in a rat model. Materials and methods: A bone cancer pain rat model was established by inoculating Walker 256 rat carcinoma cells directly into the right tibial medullary cavity of Sprague-Dawley rats (150-170 g); Phosphate buffered saline (PBS) tibial inoculation was used as control. Cancer-bearing rats were treated twice a day with external TLSJ gel (0.5 g/cm2/day) or inert gel control for 21 day (n=10/group). Behavioral tests such as mechanical threshold and paw withdrawal latency (PWL) were carried out. Osteoclastic activities were determined and carboxyterminal pyridinoline cross-linked type I collagen telopeptides (ICTP) and bone-specific alkaline phosphatase (BAP) concentrations were detected with ELISA after treatment. Adverse effects were monitored, and biochemical and histological tests were performed in naïve rats treated with local TLSJ gel for six weeks. Results: TLSJ treatment significantly restored bone cancer-induced decrease of PWL and mechanical threshold compared to inert gel. It also decreased the level of blood serum ICTP and BAP and inhibited osteoclast activities. No adverse effects or abnormal biochemical and histological changes were detected after TLSJ treatment. Conclusion: The present study shows that TLSJ significantly inhibits bone cancer-induced thermal and mechanical sensitization. It suggests that the gel may be useful in managing cancer pain and that it may act by inhibiting osteoclastic activity. © 2012 Elsevier Ireland Ltd. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm | en_US |
dc.relation.ispartof | Journal of Ethnopharmacology | en_US |
dc.subject | Bone cancer pain | - |
dc.subject | Bone-specific alkaline phosphatase | - |
dc.subject | Chinese herbs | - |
dc.subject | Osteoclast | - |
dc.subject | Tong-Luo-San-Jie (TLSJ) gel | - |
dc.subject | Type I collagen carboxy-terminal telopeptide | - |
dc.subject.mesh | Alkaline Phosphatase - Blood | en_US |
dc.subject.mesh | Analgesics - Administration & Dosage | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Bone Neoplasms - Complications - Drug Therapy | en_US |
dc.subject.mesh | Carcinoma 256, Walker - Complications - Drug Therapy | en_US |
dc.subject.mesh | Collagen Type I - Blood | en_US |
dc.subject.mesh | Drugs, Chinese Herbal - Administration & Dosage | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gels | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Osteoclasts - Drug Effects | en_US |
dc.subject.mesh | Pain - Drug Therapy - Etiology | en_US |
dc.subject.mesh | Peptides - Blood | en_US |
dc.subject.mesh | Phytotherapy | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.title | Topical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in rats | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lao, L: lxlao1@hku.hk | en_US |
dc.identifier.authority | Lao, L=rp01784 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.jep.2012.08.026 | en_US |
dc.identifier.pmid | 22960543 | - |
dc.identifier.scopus | eid_2-s2.0-84866731652 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-84866731652&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 143 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 905 | en_US |
dc.identifier.epage | 913 | en_US |
dc.identifier.isi | WOS:000309990000018 | - |
dc.publisher.place | Ireland | en_US |
dc.identifier.scopusauthorid | Wang, J=11739667100 | en_US |
dc.identifier.scopusauthorid | Zhang, R=7404864527 | en_US |
dc.identifier.scopusauthorid | Dong, C=55233377100 | en_US |
dc.identifier.scopusauthorid | Jiao, L=55350607300 | en_US |
dc.identifier.scopusauthorid | Xu, L=36524242100 | en_US |
dc.identifier.scopusauthorid | Liu, J=54684437300 | en_US |
dc.identifier.scopusauthorid | Wang, Z=8312649700 | en_US |
dc.identifier.scopusauthorid | Mao Ying, QL=13806075200 | en_US |
dc.identifier.scopusauthorid | Fong, H=55351534100 | en_US |
dc.identifier.scopusauthorid | Lao, L=7005681883 | en_US |
dc.identifier.issnl | 0378-8741 | - |