File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.neuroscience.2008.04.072
- Scopus: eid_2-s2.0-45849117181
- PMID: 18554806
- WOS: WOS:000257677900036
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Interleukin 1β facilitates bone cancer pain in rats by enhancing NMDA receptor NR-1 subunit phosphorylation
Title | Interleukin 1β facilitates bone cancer pain in rats by enhancing NMDA receptor NR-1 subunit phosphorylation |
---|---|
Authors | |
Keywords | cancer pain hyperalgesia IL-1β NMDA receptor phosphorylation spinal cord |
Issue Date | 2008 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience |
Citation | Neuroscience, 2008, v. 154 n. 4, p. 1533-1538 How to Cite? |
Abstract | It has been shown that interleukin-1β (IL-1β) facilitates nociception during neuropathic and inflammatory pain, but its involvement in bone cancer pain and its mechanisms have not previously been established. This study is an investigation of IL-1β spinal expression and the N-methyl-D-aspartate (NMDA) receptor (NMDAR) NR1 subunit phosphorylation during cancer pain, co-localization of IL-1 receptor type I (IL-1RI) and NMDAR in the spinal cord, and the effects of IL-1 receptor antagonist (IL-1ra) on NMDAR1 (NR1) phosphorylation and hyperalgesia in a rat model of bone cancer pain. Cancer was induced by injecting AT-3.1 prostate cancer cells into the tibia of the male Copenhagen rat. Phosphorylation of NR1, an essential subunit of the NMDAR, is known to modulate NMDAR activity and facilitate pain. Mechanical hyperalgesia, established by a decrease in paw withdrawal pressure threshold (PWPT), was measured at baseline and 2 h after IL-1ra treatment. IL-1ra was given (i.t.) daily for 7 days between days 13 and 19 after the cancer cell inoculation. Spinal cords were removed for Western blot to measure IL-1β and NR1 phosphorylation and for double immunostaining of IL-1RI and NR1. The data showed that 1) spinal IL-1β was up-regulated and NR1 phosphorylation was increased, 2) IL-1ra at 0.1 mg/rat significantly (P<0.05) inhibited mechanical hyperalgesia, increasing PWPT on day 14 from 71.1±3.1-85.3±4.6 g and on day 19 from 73.5.0±3.5-87.1±3.7 g, and inhibited NR1 phosphorylation compared with saline control, and 3) IL-1RI is localized in NR1-immunoreactive neurons within the spinal cord. The results suggest that spinal IL-1β enhances NR1 phosphorylation to facilitate bone cancer pain. © 2008 IBRO. |
Persistent Identifier | http://hdl.handle.net/10722/188604 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.903 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, RX | en_US |
dc.contributor.author | Liu, B | en_US |
dc.contributor.author | Li, A | en_US |
dc.contributor.author | Wang, L | en_US |
dc.contributor.author | Ren, K | en_US |
dc.contributor.author | Qiao, JT | en_US |
dc.contributor.author | Berman, BM | en_US |
dc.contributor.author | Lao, L | en_US |
dc.date.accessioned | 2013-09-03T04:10:32Z | - |
dc.date.available | 2013-09-03T04:10:32Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Neuroscience, 2008, v. 154 n. 4, p. 1533-1538 | en_US |
dc.identifier.issn | 0306-4522 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/188604 | - |
dc.description.abstract | It has been shown that interleukin-1β (IL-1β) facilitates nociception during neuropathic and inflammatory pain, but its involvement in bone cancer pain and its mechanisms have not previously been established. This study is an investigation of IL-1β spinal expression and the N-methyl-D-aspartate (NMDA) receptor (NMDAR) NR1 subunit phosphorylation during cancer pain, co-localization of IL-1 receptor type I (IL-1RI) and NMDAR in the spinal cord, and the effects of IL-1 receptor antagonist (IL-1ra) on NMDAR1 (NR1) phosphorylation and hyperalgesia in a rat model of bone cancer pain. Cancer was induced by injecting AT-3.1 prostate cancer cells into the tibia of the male Copenhagen rat. Phosphorylation of NR1, an essential subunit of the NMDAR, is known to modulate NMDAR activity and facilitate pain. Mechanical hyperalgesia, established by a decrease in paw withdrawal pressure threshold (PWPT), was measured at baseline and 2 h after IL-1ra treatment. IL-1ra was given (i.t.) daily for 7 days between days 13 and 19 after the cancer cell inoculation. Spinal cords were removed for Western blot to measure IL-1β and NR1 phosphorylation and for double immunostaining of IL-1RI and NR1. The data showed that 1) spinal IL-1β was up-regulated and NR1 phosphorylation was increased, 2) IL-1ra at 0.1 mg/rat significantly (P<0.05) inhibited mechanical hyperalgesia, increasing PWPT on day 14 from 71.1±3.1-85.3±4.6 g and on day 19 from 73.5.0±3.5-87.1±3.7 g, and inhibited NR1 phosphorylation compared with saline control, and 3) IL-1RI is localized in NR1-immunoreactive neurons within the spinal cord. The results suggest that spinal IL-1β enhances NR1 phosphorylation to facilitate bone cancer pain. © 2008 IBRO. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience | en_US |
dc.relation.ispartof | Neuroscience | en_US |
dc.subject | cancer pain | - |
dc.subject | hyperalgesia | - |
dc.subject | IL-1β | - |
dc.subject | NMDA receptor | - |
dc.subject | phosphorylation | - |
dc.subject | spinal cord | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antirheumatic Agents - Pharmacology | en_US |
dc.subject.mesh | Blotting, Western | en_US |
dc.subject.mesh | Bone Neoplasms - Complications | en_US |
dc.subject.mesh | Fluorescent Antibody Technique | en_US |
dc.subject.mesh | Hyperalgesia - Physiopathology | en_US |
dc.subject.mesh | Immunohistochemistry | en_US |
dc.subject.mesh | Interleukin 1 Receptor Antagonist Protein - Pharmacology | en_US |
dc.subject.mesh | Interleukin-1Beta - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Neurons - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Pain - Etiology - Physiopathology | en_US |
dc.subject.mesh | Phosphorylation | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Receptors, N-Methyl-D-Aspartate - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Spinal Cord - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Up-Regulation | en_US |
dc.title | Interleukin 1β facilitates bone cancer pain in rats by enhancing NMDA receptor NR-1 subunit phosphorylation | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lao, L: lxlao1@hku.hk | en_US |
dc.identifier.authority | Lao, L=rp01784 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.neuroscience.2008.04.072 | en_US |
dc.identifier.pmid | 18554806 | - |
dc.identifier.scopus | eid_2-s2.0-45849117181 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-45849117181&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 154 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 1533 | en_US |
dc.identifier.epage | 1538 | en_US |
dc.identifier.isi | WOS:000257677900036 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Zhang, RX=7404864527 | en_US |
dc.identifier.scopusauthorid | Liu, B=55720712900 | en_US |
dc.identifier.scopusauthorid | Li, A=16245342100 | en_US |
dc.identifier.scopusauthorid | Wang, L=9036448600 | en_US |
dc.identifier.scopusauthorid | Ren, K=7102272533 | en_US |
dc.identifier.scopusauthorid | Qiao, JT=7103301572 | en_US |
dc.identifier.scopusauthorid | Berman, BM=35458606800 | en_US |
dc.identifier.scopusauthorid | Lao, L=7005681883 | en_US |
dc.identifier.issnl | 0306-4522 | - |