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- Publisher Website: 10.1002/pros.2990220108
- Scopus: eid_2-s2.0-0027535976
- PMID: 8426838
- WOS: WOS:A1993KJ89300007
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Article: High-affinity L-aspartate transporter in prostate epithelial cells that is regulated by testosterone
Title | High-affinity L-aspartate transporter in prostate epithelial cells that is regulated by testosterone |
---|---|
Authors | |
Keywords | active transport citrate glutamate ventral prostate |
Issue Date | 1993 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304 |
Citation | Prostate, 1993, v. 22 n. 1, p. 53-63 How to Cite? |
Abstract | The prostate gland produces and secretes extraordinarily high levels of citrate. Studies with rat ventral prostate (VP) have demonstrated that aspartate can serve as a four-carbon source of oxalacetate in the synthesis of citrate. To achieve this, prostate secretory epithelial cells must contain a transport system for the active uptake of aspartate from circulation. The present studies with VP epithelial cells confirm the existence of a Na +-dependent high affinity L-aspartate transporter. The transporter has an optimal pH ~ 7.5 and is temperature dependent. It appears to be an anionic amino acid transporter capable of transporting L-glutamate but not basic or neutral amino acids. The transporter is inhibited by ATPase inhibitors, thereby indicating its dependency on a Na + gradient. The characteristics of the high-affinity L-aspartate transporter are consistent with its operation at the basilar membrane for the transport of circulating aspartate into the cell. Castration (24 hr) resulted in a significant decrease in the ability of VP epithelial cells to transport L-aspartate. The administration of testosterone to castrated rats completely restored L-aspartate transport. In addition, in vitro testosterone addition (10 -8 M for 30 min) to isolated prostate epithelial cells markedly increased L-aspartate transport. Both cycloheximide and actinomycin inhibited the testosterone effect. The studies reveal that testosterone is a regulator of this Na +-dependent high-affinity L-aspartate transporter. The mechanism of this testosterone effect appears to involve both RNA and protein synthesis. We now have a model system to elucidate this novel effect of testosterone. |
Persistent Identifier | http://hdl.handle.net/10722/188524 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 1.032 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lao, L | en_US |
dc.contributor.author | Franklin, RB | en_US |
dc.contributor.author | Costello, LC | en_US |
dc.date.accessioned | 2013-09-03T04:10:05Z | - |
dc.date.available | 2013-09-03T04:10:05Z | - |
dc.date.issued | 1993 | en_US |
dc.identifier.citation | Prostate, 1993, v. 22 n. 1, p. 53-63 | en_US |
dc.identifier.issn | 0270-4137 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/188524 | - |
dc.description.abstract | The prostate gland produces and secretes extraordinarily high levels of citrate. Studies with rat ventral prostate (VP) have demonstrated that aspartate can serve as a four-carbon source of oxalacetate in the synthesis of citrate. To achieve this, prostate secretory epithelial cells must contain a transport system for the active uptake of aspartate from circulation. The present studies with VP epithelial cells confirm the existence of a Na +-dependent high affinity L-aspartate transporter. The transporter has an optimal pH ~ 7.5 and is temperature dependent. It appears to be an anionic amino acid transporter capable of transporting L-glutamate but not basic or neutral amino acids. The transporter is inhibited by ATPase inhibitors, thereby indicating its dependency on a Na + gradient. The characteristics of the high-affinity L-aspartate transporter are consistent with its operation at the basilar membrane for the transport of circulating aspartate into the cell. Castration (24 hr) resulted in a significant decrease in the ability of VP epithelial cells to transport L-aspartate. The administration of testosterone to castrated rats completely restored L-aspartate transport. In addition, in vitro testosterone addition (10 -8 M for 30 min) to isolated prostate epithelial cells markedly increased L-aspartate transport. Both cycloheximide and actinomycin inhibited the testosterone effect. The studies reveal that testosterone is a regulator of this Na +-dependent high-affinity L-aspartate transporter. The mechanism of this testosterone effect appears to involve both RNA and protein synthesis. We now have a model system to elucidate this novel effect of testosterone. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304 | en_US |
dc.relation.ispartof | Prostate | en_US |
dc.subject | active transport | - |
dc.subject | citrate | - |
dc.subject | glutamate | - |
dc.subject | ventral prostate | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aspartic Acid - Metabolism | en_US |
dc.subject.mesh | Biological Transport, Active - Drug Effects | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Epithelium - Metabolism | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Prostate - Metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Wistar | en_US |
dc.subject.mesh | Sodium - Metabolism | en_US |
dc.subject.mesh | Temperature | en_US |
dc.subject.mesh | Testosterone - Pharmacology | en_US |
dc.title | High-affinity L-aspartate transporter in prostate epithelial cells that is regulated by testosterone | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lao, L: lxlao1@hku.hk | en_US |
dc.identifier.authority | Lao, L=rp01784 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/pros.2990220108 | - |
dc.identifier.pmid | 8426838 | en_US |
dc.identifier.scopus | eid_2-s2.0-0027535976 | en_US |
dc.identifier.volume | 22 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 53 | en_US |
dc.identifier.epage | 63 | en_US |
dc.identifier.isi | WOS:A1993KJ89300007 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lao, L=7005681883 | en_US |
dc.identifier.scopusauthorid | Franklin, RB=7201428474 | en_US |
dc.identifier.scopusauthorid | Costello, LC=24592383900 | en_US |
dc.identifier.issnl | 0270-4137 | - |