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Article: Genomic sequence based scanning for drug resistance-associated mutations and evolutionary analysis of multidrug-resistant and extensively drug-resistance Mycobacterium tuberculosis

TitleGenomic sequence based scanning for drug resistance-associated mutations and evolutionary analysis of multidrug-resistant and extensively drug-resistance Mycobacterium tuberculosis
Authors
Issue Date2012
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jinf
Citation
Journal of Infection, 2012, v. 65 n. 5, p. 412-422 How to Cite?
AbstractOBJECTIVE: To better understand the molecular mechanisms and evolution of drug resistance in Mycobacterium tuberculosis (M. tuberculosis), we performed a genomic sequence based scanning of drug resistance-associated loci for multidrug-resistant (MDR) and extensively drug-resistant (XDR) M. tuberculosis strains. MATERIALS AND METHODS: Forty-five pairs of primers covering known drug resistance-associated loci compiled in the TBDReaMDB database were designed to perform the analysis of drug resistance-associated mutations for 14 M. tuberculosis clinical isolates from TB patients in China. Genetic diversity and evolutionary analysis was done using concatenated nucleotide sequences of drug resistance-associated loci. RESULTS: Forty-four types of mutations were identified in 14 M. tuberculosis clinical isolates. Average nucleotide diversity for drug resistance-associated loci increased in the M. tuberculosis isolates as the drug resistance increased (pi = 0, pi = 0.00021, and pi = 0.00028 for susceptible, MDR, and XDR isolates, respectively). The dN/dS ratios for coding regions of drug resistance-associated genes in MDR and XDR isolates were 2.73 and 1.83, respectively. MDR and XDR isolates were distributed sporadically on different branches in the phylogenetic trees. CONCLUSIONS: Our study provides supporting evidence to demonstrate that the MDR- and XDR-M. tuberculosis strains have evolved independently driven by positive selection.
Persistent Identifierhttp://hdl.handle.net/10722/184605
ISSN
2023 Impact Factor: 14.3
2023 SCImago Journal Rankings: 2.669
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, CH-
dc.contributor.authorLi, HM-
dc.contributor.authorLu, N-
dc.contributor.authorWang, Q-
dc.contributor.authorHu, YL-
dc.contributor.authorYang, X-
dc.contributor.authorHu, YF-
dc.contributor.authorWoo, PCY-
dc.contributor.authorGao, GF-
dc.contributor.authorZhu, B-
dc.date.accessioned2013-07-15T09:57:37Z-
dc.date.available2013-07-15T09:57:37Z-
dc.date.issued2012-
dc.identifier.citationJournal of Infection, 2012, v. 65 n. 5, p. 412-422-
dc.identifier.issn0163-4453-
dc.identifier.urihttp://hdl.handle.net/10722/184605-
dc.description.abstractOBJECTIVE: To better understand the molecular mechanisms and evolution of drug resistance in Mycobacterium tuberculosis (M. tuberculosis), we performed a genomic sequence based scanning of drug resistance-associated loci for multidrug-resistant (MDR) and extensively drug-resistant (XDR) M. tuberculosis strains. MATERIALS AND METHODS: Forty-five pairs of primers covering known drug resistance-associated loci compiled in the TBDReaMDB database were designed to perform the analysis of drug resistance-associated mutations for 14 M. tuberculosis clinical isolates from TB patients in China. Genetic diversity and evolutionary analysis was done using concatenated nucleotide sequences of drug resistance-associated loci. RESULTS: Forty-four types of mutations were identified in 14 M. tuberculosis clinical isolates. Average nucleotide diversity for drug resistance-associated loci increased in the M. tuberculosis isolates as the drug resistance increased (pi = 0, pi = 0.00021, and pi = 0.00028 for susceptible, MDR, and XDR isolates, respectively). The dN/dS ratios for coding regions of drug resistance-associated genes in MDR and XDR isolates were 2.73 and 1.83, respectively. MDR and XDR isolates were distributed sporadically on different branches in the phylogenetic trees. CONCLUSIONS: Our study provides supporting evidence to demonstrate that the MDR- and XDR-M. tuberculosis strains have evolved independently driven by positive selection.-
dc.languageeng-
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jinf-
dc.relation.ispartofJournal of Infection-
dc.subject.meshDNA Mutational Analysis-
dc.subject.meshDrug Resistance, Multiple, Bacterial - genetics-
dc.subject.meshExtensively Drug-Resistant Tuberculosis - microbiology-
dc.subject.meshMycobacterium tuberculosis - classification - drug effects - genetics-
dc.subject.meshTuberculosis, Multidrug-Resistant - microbiology-
dc.titleGenomic sequence based scanning for drug resistance-associated mutations and evolutionary analysis of multidrug-resistant and extensively drug-resistance Mycobacterium tuberculosis-
dc.typeArticle-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jinf.2012.06.007-
dc.identifier.pmid22728171-
dc.identifier.scopuseid_2-s2.0-84867330815-
dc.identifier.hkuros216419-
dc.identifier.volume65-
dc.identifier.issue5-
dc.identifier.spage412-
dc.identifier.epage422-
dc.identifier.isiWOS:000310782300006-
dc.publisher.placeUnited Kingdom-

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